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Details of TopicalPdb ID 1026

PRIMARY INFORMATION
ID1026
PMID23311648
Year2013
SequenceGRKKRRQRRRPPQRKC
NameTat
Length16
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideHIV-1
Nature of Peptide/CargoCell penetrating peptide
MechanismTat enters cells by macropinocytosis, a specialized form of fluid-phase endocytosis that occurs in all cells
Cargo Sequence/StructureNot mentioned
Name of cargo
Human tyrosinase plasmid (pAH7/Tyr, P)
AssayVertical Franz diffusion cell
EnhancerElastic cationic niosomes
Properties of enhancerElastic niosome can squeeze themselves and pass through a small pore in stratum corneum, which is smaller than their vesicular size
Concentration1g of gel formulation containing 30 µg of tyrosinase plasmid, 20µl of trypsin solution to hydrolyze the Tat
Incubation time6 hours
Tissue permeability (value with units)Cumulative amounts 1.02 ± 0.05 µg/cm2 and fluxes 6.13± 0.28 µg/cm2·h
Tissue SampleAcceptor compartment of Franz diffusion cell/Abdominal skin of Sprague–Dawley rats
Ex vivo/In vivo/In vitroin vitro
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESNCC(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)
N[C@@H](CCCC[NH3])C(=O)N[C@@H](CCCC
[NH3])C(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)
N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H](CCC(=O)
N)C(=O)N[C@@H](CCCNC(=[NH2])N)C(=O)N[C@@H]
(CCCNC(=[NH2])N)C(=O)N[C@@H](CCCNC
(=[NH2])N)C(=O)N1CCC[C@H]1C(=O)N1CCC
[C@H]1C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H]
(CCCNC(=[NH2])N)C(=O)N[C@@H](CCCC[NH3])C
(=O)N[C@@H](CS)C=O