NPACT- Naturally occuring Plant based Anticancerous Compound-Activity-Target DataBase

Compound: Pinocembrin

TargetsGene NameGene IDCancerCell LinesIC50ED50EC50GI50RemarkReferences
BAXBCL2-associated X protein581Colon cancerHCT-116----Pinocembrin (100 mµM) induced Bax translocation to mitochondria. Overexpression of Bax sensitized cells to pinocembrin-induced apoptosis as evidenced from MTT and chromatin condensation.17186548
BAXBCL2-associated X protein581Neuroblastoma SH-SY5Y----Pinocembrin treatment reduced the expression of Bax and had no effect on Bcl-2, thus decreased the Bax-Bcl-2 ratio.18625218
BCL2L1BCL2-like 1598Colon cancerHCT-116----Here,it is indicated that cytochrome c release induced by pinocembrin is not blocked by Bcl-XL. And overexpression of Bcl-XL only partially prevented chromatin condensation and cytochrome c release induced by pinocembrin compared to vector-transfected cells.17186548
CASP3caspase 3, apoptosis-related cysteine peptidase836Colon cancerHCT-116----The processing of caspase-3 was evident from 24 h of pinocembrin treatment and by 48 hmore intense cleavage fragment was evident. Caspase-3 inhibitor reduced the chromatin condensation to 15%, compared to 30% in DMSO control.17186548
CASP9caspase 9, apoptosis-related cysteine peptidase842Colon cancerHCT-116----Caspase-9 was cleaved to its cleavage fragment (37 kDa) after 48 h of pinocembrin treatment.Pinocembrin-induced caspase-9 processing is subsequent to loss of mitochondrial membrane permeabilization and release of cytochrome c into cytosol, indicating a possible role for classical mitochondrial apoptotic signaling. Caspase-9 inhibitor reduced the chromatin condensation to 18%, compared to 30% in DMSO control.17186548
CYCScytochrome c54205Neuroblastoma SH-SY5Y----Pinocembrin inhibit the release of cytochrome c from mitochondria to cytosol.18625218
CYCScytochrome c54205Colon cancerHCT-116----The release of cytochrome c is essential for the processing of initiator caspase-9 in mitochondria-mediated apoptotic signaling. The release of cytochrome c in to cytosol began after 24 h of pinocembrin and gradually increased to 72 h.17186548
PARP1poly (ADP-ribose) polymerase 1142Colon cancerHCT-116----PARP is an important enzyme responsible for the DNA repair and is a preferred substrate for processed caspase-7 and -3.Western blot analysis of treated samples shows that cleavage of PARP is evident only around 48 h of drug exposure.17186548
TP53tumor protein p537157Neuroblastoma SH-SY5Y----Pinocembrin could also down-regulate the expression of p53 protein, and inhibit the release of cytochrome c from mitochondria to cytosol.18625218

 






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