Welcome to Hemolytik 2.0

Hemolytik 2.0 is an extensively updated, manually curated database that stands as the premier resource for experimentally validated hemolytic and non-hemolytic peptides. It is an updated version of database Hemolytik created by our group. In this database, peptides have been collected from both published research/ review articles as well as from other database like CAMP-R4, DRAMP, APD3, and UniProt. Boasting over 13,200 fully annotated entries - encompassing sequence, biological source, structural class, stereochemistry, terminal and chemical modifications, functional characterization, and hemolytic activity - Hemolytik 2.0 uniquely provides detailed SMILES molecular representations and predictive 3D structures of peptides. These structures are predicted using PEPstrMod and secondary structure states are assigned using DSSP. We have also integrated the MAP (Modification and Annotation in Proteins) format, which enables the inclusion of tags directly within sequences to represent residue-level modifications such as chemical alterations, non-standard amino acids, binding sites, and mutations. Structure of modified peptides (containing Non-natural, D-amino acids, Modified-amino acids like Terminal modifications like Acetylation/Amidation) have also been predicted. The platform’s RESTful API enables To facilitate automated data access, Haemolytic 2.0 now includes a RESTful API. This allows users to programmatically retrieve data based on parameters such as sequence type, peptide origin, or hemolytic activity. Cutting-edge web tools, including advanced search, sequence mapping, structure alignment, and BLAST utilities, streamline peptide exploration and experimental planning. By vastly expanding peptide diversity, annotation depth, and user functionality, Hemolytik 2.0 accelerates rational peptide drug design and safety assessment, equipping researchers and developers with an indispensable asset for innovating next-generation peptide therapeutics.

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