Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     299
XP_006713721                                                           20     299
NP_000245                                                              17    4312
XP_006711832                                                           17    4312
XP_005273200                                                           17    4312
XP_005273198                                                           17    4312
NP_079334                                                              16    5631
NP_001243529                                                           16    5631
NP_001269307                                                           16    5631
NP_001243528                                                           16    5631
NP_001243530                                                           16    5631
XP_005250646                                                           16    7355
XP_005250645                                                           16    7355
XP_005250644                                                           16    7355
XP_005250643                                                           16    7355
XP_005250642                                                           16    7355
NP_077291                                                              16    7355
NP_001165907                                                           15    9605
NP_002628                                                              15    9605
NP_000159                                                              15    9605
NP_001116142                                                           15    9605
NP_001073896                                                           15    9605
NP_060276                                                              15    9605
NP_060145                                                              15    9605
NP_001075956                                                           15    9605
NP_001165447                                                           15    9605
NP_079414                                                              15    9605
NP_075065                                                              15    9605
NP_849193                                                              15    9605
XP_006724724                                                           15    9605
XP_005256172                                                           15    9605
XP_005266602                                                           15    9605
XP_005249761                                                           15    9605
XP_005249760                                                           15    9605
XP_006714950                                                           15    9605
XP_006713080                                                           15    9605
XP_005263859                                                           15    9605
NP_001075955                                                           15    9605
NP_057558                                                              15    9605
>NP_006209
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   YSNAGLSNR 9
           YS+AGLSNR
Sbjct: 508 YSHAGLSNR 516
>XP_006713721
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   YSNAGLSNR 9
           YS+AGLSNR
Sbjct: 508 YSHAGLSNR 516
>NP_000245
          Length = 1265

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   YSNAGLSN 8
           Y NAGL N
Sbjct: 285 YPNAGLPN 292
>XP_006711832
          Length = 1139

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   YSNAGLSN 8
           Y NAGL N
Sbjct: 285 YPNAGLPN 292
>XP_005273200
          Length = 1214

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   YSNAGLSN 8
           Y NAGL N
Sbjct: 285 YPNAGLPN 292
>XP_005273198
          Length = 1264

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   YSNAGLSN 8
           Y NAGL N
Sbjct: 284 YPNAGLPN 291
>NP_079334
          Length = 759

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNAGLSNR 9
           SNAGL+ R
Sbjct: 669 SNAGLTGR 676
>NP_001243529
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNAGLSNR 9
           SNAGL+ R
Sbjct: 627 SNAGLTGR 634
>NP_001269307
          Length = 633

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNAGLSNR 9
           SNAGL+ R
Sbjct: 543 SNAGLTGR 550
>NP_001243528
          Length = 736

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNAGLSNR 9
           SNAGL+ R
Sbjct: 646 SNAGLTGR 653
>NP_001243530
          Length = 746

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SNAGLSNR 9
           SNAGL+ R
Sbjct: 656 SNAGLTGR 663
>XP_005250646
          Length = 118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  YSNAGLSNR 9
          Y  +GL NR
Sbjct: 9  YGTSGLDNR 17
>XP_005250645
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  YSNAGLSNR 9
          Y  +GL NR
Sbjct: 9  YGTSGLDNR 17
>XP_005250644
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  YSNAGLSNR 9
          Y  +GL NR
Sbjct: 9  YGTSGLDNR 17
>XP_005250643
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  YSNAGLSNR 9
          Y  +GL NR
Sbjct: 9  YGTSGLDNR 17
>XP_005250642
          Length = 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  YSNAGLSNR 9
          Y  +GL NR
Sbjct: 9  YGTSGLDNR 17
>NP_077291
          Length = 118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  YSNAGLSNR 9
          Y  +GL NR
Sbjct: 9  YGTSGLDNR 17
>NP_001165907
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YSNAGLSNR 9
           YS+ G +NR
Sbjct: 483 YSSLGCNNR 491
>NP_002628
          Length = 1223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YSNAGLSNR 9
           YS+ G +NR
Sbjct: 483 YSSLGCNNR 491
>NP_000159
          Length = 1580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSNAGL 6
           YSN+GL
Sbjct: 723 YSNSGL 728
>NP_001116142
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YSNAGLSNR 9
           YS+ G +NR
Sbjct: 483 YSSLGCNNR 491
>NP_001073896
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SNAGLSNR 9
          SN G SNR
Sbjct: 48 SNCGESNR 55
>NP_060276
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSNAGLS 7
           YSN GL+
Sbjct: 145 YSNVGLA 151
>NP_060145
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    YSNAGL 6
            Y+NAGL
Sbjct: 1300 YTNAGL 1305
>NP_001075956
          Length = 528

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SNAGLS 7
           SNAGLS
Sbjct: 245 SNAGLS 250
>NP_001165447
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    YSNAGL 6
            Y+NAGL
Sbjct: 1300 YTNAGL 1305
>NP_079414
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSNAGL 6
           Y+NAGL
Sbjct: 217 YNNAGL 222
>NP_075065
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SNAGLS 7
           SNAGLS
Sbjct: 245 SNAGLS 250
>NP_849193
          Length = 826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YSNAGLSNR 9
           Y  AG++NR
Sbjct: 609 YQIAGMANR 617
>XP_006724724
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YSNAGLSNR 9
           YS+ G +NR
Sbjct: 483 YSSLGCNNR 491
>XP_005256172
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SNAGLS 7
           SNAGLS
Sbjct: 248 SNAGLS 253
>XP_005266602
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSNAGL 6
           Y+NAGL
Sbjct: 195 YNNAGL 200
>XP_005249761
          Length = 1580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSNAGL 6
           YSN+GL
Sbjct: 723 YSNSGL 728
>XP_005249760
          Length = 1580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSNAGL 6
           YSN+GL
Sbjct: 723 YSNSGL 728
>XP_006714950
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    YSNAGL 6
            Y+NAGL
Sbjct: 1294 YTNAGL 1299
>XP_006713080
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YSNAGLSNR 9
           Y  AG++NR
Sbjct: 609 YQIAGMANR 617
>XP_005263859
          Length = 1606

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  SNAGLSNR 9
          SN G SNR
Sbjct: 79 SNCGESNR 86
>NP_001075955
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SNAGLS 7
           SNAGLS
Sbjct: 248 SNAGLS 253
>NP_057558
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  NAGLSNR 9
          N G+SNR
Sbjct: 58 NVGVSNR 64
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.119    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 520,652
Number of extensions: 784
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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