Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060872                                                              18    1135
NP_659206                                                              18    1482
NP_659205                                                              18    1482
NP_006790                                                              18    1482
NP_004680                                                              17    3301
NP_001254483                                                           17    4312
NP_689716                                                              17    4312
NP_000122                                                              17    4312
NP_062562                                                              17    4312
XP_006720026                                                           17    4312
NP_001181                                                              16    5631
NP_777576                                                              16    5631
NP_060733                                                              16    5631
NP_055634                                                              16    5631
NP_071342                                                              16    7355
NP_001094282                                                           16    7355
NP_001005512                                                           16    7355
NP_892018                                                              16    7355
XP_005259608                                                           16    7355
XP_006719430                                                           16    7355
XP_006719428                                                           16    7355
XP_005253933                                                           16    7355
XP_006718293                                                           16    7355
XP_006714288                                                           16    7355
NP_001084                                                              16    7355
NP_001188478                                                           15    9605
NP_004687                                                              15    9605
NP_001188475                                                           15    9605
NP_001165903                                                           15    9605
NP_975010                                                              15    9605
NP_006828                                                              15    9605
NP_005038                                                              15    9605
NP_079416                                                              15    9605
NP_001712                                                              15    9605
NP_001257356                                                           15    9605
XP_006724573                                                           15    9605
XP_005253682                                                           15    9605
XP_006719674                                                           15    9605
XP_005253681                                                           15    9605
XP_005245481                                                           15    9605
XP_005271374                                                           15    9605
>NP_060872
          Length = 171

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WIRKLMSSS 9
           WI+KL+ SS
Sbjct: 162 WIKKLLESS 170
>NP_659206
          Length = 300

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WIRKLMSSS 9
           WI+KLM+ S
Sbjct: 266 WIQKLMAQS 274
>NP_659205
          Length = 312

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WIRKLMSSS 9
           WI+KLM+ S
Sbjct: 278 WIQKLMAQS 286
>NP_006790
          Length = 314

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WIRKLMSSS 9
           WI+KLM+ S
Sbjct: 280 WIQKLMAQS 288
>NP_004680
          Length = 715

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   IRKLMSSS 9
           IRKL+SSS
Sbjct: 668 IRKLLSSS 675
>NP_001254483
          Length = 382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   WIRKLMSS 8
           W++KLM S
Sbjct: 362 WLQKLMRS 369
>NP_689716
          Length = 1052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    WIRKLMSSS 9
            W++KLM  S
Sbjct: 1042 WLQKLMGRS 1050
>NP_000122
          Length = 466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   WIRKLMSS 8
           W++KLM S
Sbjct: 446 WLQKLMRS 453
>NP_062562
          Length = 444

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   WIRKLMSS 8
           W++KLM S
Sbjct: 424 WLQKLMRS 431
>XP_006720026
          Length = 397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   WIRKLMSS 8
           W++KLM S
Sbjct: 377 WLQKLMRS 384
>NP_001181
          Length = 392

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  WIRKLMS 7
          W RKL+S
Sbjct: 10 WARKLLS 16
>NP_777576
          Length = 1749

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   WIRKLMSSS 9
           W+ K+MS S
Sbjct: 320 WMNKIMSYS 328
>NP_060733
          Length = 1435

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   WIRKLMSSS 9
           W RK+ SSS
Sbjct: 131 WKRKVSSSS 139
>NP_055634
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IRKLMSSS 9
           +RKL+SSS
Sbjct: 318 VRKLISSS 325
>NP_071342
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   WIRKLMS 7
           W+++LMS
Sbjct: 108 WVQELMS 114
>NP_001094282
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   WIRKLMS 7
           W+++LMS
Sbjct: 108 WVQELMS 114
>NP_001005512
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   WIRKLMSSS 9
           W R L+SSS
Sbjct: 300 WRRDLISSS 308
>NP_892018
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   WIRKLMSS 8
           WI ++MSS
Sbjct: 730 WILEIMSS 737
>XP_005259608
          Length = 1093

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   WIRKLMSS 8
           WI ++MSS
Sbjct: 764 WILEIMSS 771
>XP_006719430
          Length = 2256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   WIRKLM 6
           W++KLM
Sbjct: 822 WVQKLM 827
>XP_006719428
          Length = 2458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    WIRKLM 6
            W++KLM
Sbjct: 1024 WVQKLM 1029
>XP_005253933
          Length = 2458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    WIRKLM 6
            W++KLM
Sbjct: 1024 WVQKLM 1029
>XP_006718293
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   WIRKLMSSS 9
           W R L+SSS
Sbjct: 300 WRRDLISSS 308
>XP_006714288
          Length = 183

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  WIRKLM 6
          W+RKL+
Sbjct: 8  WLRKLL 13
>NP_001084
          Length = 2458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1    WIRKLM 6
            W++KLM
Sbjct: 1024 WVQKLM 1029
>NP_001188478
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WIRKLMSS 8
          WI  +MSS
Sbjct: 61 WIGSIMSS 68
>NP_004687
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WIRKLMSS 8
          WI  +MSS
Sbjct: 61 WIGSIMSS 68
>NP_001188475
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WIRKLMSS 8
          WI  +MSS
Sbjct: 61 WIGSIMSS 68
>NP_001165903
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WIRKLMS 7
           WI KLM+
Sbjct: 366 WIGKLMA 372
>NP_975010
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRKLMSSS 9
           +RKL SSS
Sbjct: 246 VRKLKSSS 253
>NP_006828
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   WIRKLMSSS 9
           W+  L SSS
Sbjct: 246 WVNTLSSSS 254
>NP_005038
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   WIRKLMSSS 9
           W +KLM +S
Sbjct: 426 WAQKLMFNS 434
>NP_079416
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKLMSSS 9
           +KLMSSS
Sbjct: 167 KKLMSSS 173
>NP_001712
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRKLMSSS 9
           +RKL SSS
Sbjct: 246 VRKLKSSS 253
>NP_001257356
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   WIRKLMSSS 9
           W +KLM +S
Sbjct: 383 WAQKLMFNS 391
>XP_006724573
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRKLMSSS 9
           +RKL SSS
Sbjct: 246 VRKLKSSS 253
>XP_005253682
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKLMSSS 9
           +KLMSSS
Sbjct: 167 KKLMSSS 173
>XP_006719674
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKLMSSS 9
           +KLMSSS
Sbjct: 167 KKLMSSS 173
>XP_005253681
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RKLMSSS 9
           +KLMSSS
Sbjct: 167 KKLMSSS 173
>XP_005245481
          Length = 512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  WIRKLMSS 8
          W RKL+++
Sbjct: 5  WARKLLAA 12
>XP_005271374
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WIRKLMSS 8
          WI  +MSS
Sbjct: 61 WIGSIMSS 68
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.119    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 425,293
Number of extensions: 815
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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