Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167571                                                           18    1135
NP_127503                                                              18    1135
NP_127501                                                              18    1135
NP_065151                                                              18    1135
XP_006713333                                                           18    1135
XP_005265389                                                           18    1135
XP_005265388                                                           18    1135
XP_006713332                                                           18    1135
XP_006713331                                                           18    1135
XP_006713330                                                           18    1135
XP_006713329                                                           18    1135
XP_005265387                                                           18    1135
XP_006711904                                                           17    2528
NP_003822                                                              17    2528
NP_002833                                                              17    3301
NP_001154912                                                           17    3301
XP_006723375                                                           17    3301
NP_004987                                                              17    4312
XP_006720948                                                           17    4312
XP_006720947                                                           17    4312
XP_005255386                                                           17    4312
XP_005255385                                                           17    4312
XP_005255384                                                           17    4312
XP_006710061                                                           17    4312
XP_006710060                                                           17    4312
NP_057530                                                              16    5631
NP_689594                                                              16    5631
NP_065928                                                              16    5631
NP_065831                                                              16    5631
XP_005256527                                                           16    5631
XP_006712319                                                           16    5631
NP_001186073                                                           16    5631
NP_001027563                                                           16    5631
NP_057393                                                              16    5631
NP_000789                                                              16    7355
NP_031375                                                              16    7355
XP_005276205                                                           16    7355
XP_005249593                                                           16    7355
XP_005277516                                                           16    7355
NP_001026                                                              15    9605
NP_061027                                                              15    9605
NP_060501                                                              15    9605
NP_689414                                                              15    9605
NP_001154994                                                           15    9605
NP_009177                                                              15    9605
NP_001154993                                                           15    9605
XP_005262767                                                           15    9605
XP_006714137                                                           15    9605
XP_006711873                                                           15    9605
XP_006711872                                                           15    9605
XP_006711871                                                           15    9605
XP_006711870                                                           15    9605
XP_006711869                                                           15    9605
XP_006711868                                                           15    9605
XP_006711867                                                           15    9605
XP_006711866                                                           15    9605
XP_006711865                                                           15    9605
>NP_001167571
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 305 ITACLETAK 313
>NP_127503
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 305 ITACLETAK 313
>NP_127501
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 305 ITACLETAK 313
>NP_065151
          Length = 360

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 262 ITACLETAK 270
>XP_006713333
          Length = 360

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 262 ITACLETAK 270
>XP_005265389
          Length = 383

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 285 ITACLETAK 293
>XP_005265388
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 305 ITACLETAK 313
>XP_006713332
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 305 ITACLETAK 313
>XP_006713331
          Length = 403

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 305 ITACLETAK 313
>XP_006713330
          Length = 424

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 326 ITACLETAK 334
>XP_006713329
          Length = 424

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 326 ITACLETAK 334
>XP_005265387
          Length = 426

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           +TACL T+K
Sbjct: 328 ITACLETAK 336
>XP_006711904
          Length = 630

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  VTACLSTSK 9
          V++CLSTS+
Sbjct: 36 VSSCLSTSQ 44
>NP_003822
          Length = 519

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VTACLSTSK 9
           +T C+ST K
Sbjct: 253 ITGCISTGK 261
>NP_002833
          Length = 1115

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VTACLSTS 8
           +T+C+STS
Sbjct: 670 ITSCVSTS 677
>NP_001154912
          Length = 937

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VTACLSTS 8
           +T+C+STS
Sbjct: 492 ITSCVSTS 499
>XP_006723375
          Length = 1137

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VTACLSTS 8
           +T+C+STS
Sbjct: 692 ITSCVSTS 699
>NP_004987
          Length = 1531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           VTACL T
Sbjct: 372 VTACLQT 378
>XP_006720948
          Length = 1401

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           VTACL T
Sbjct: 242 VTACLQT 248
>XP_006720947
          Length = 1466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           VTACL T
Sbjct: 372 VTACLQT 378
>XP_005255386
          Length = 1472

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           VTACL T
Sbjct: 372 VTACLQT 378
>XP_005255385
          Length = 1485

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           VTACL T
Sbjct: 326 VTACLQT 332
>XP_005255384
          Length = 1489

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           VTACL T
Sbjct: 330 VTACLQT 336
>XP_006710061
          Length = 298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TACLST 7
           TACLST
Sbjct: 267 TACLST 272
>XP_006710060
          Length = 425

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TACLST 7
           TACLST
Sbjct: 394 TACLST 399
>NP_057530
          Length = 338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VTACLSTS 8
           VT C+ST+
Sbjct: 291 VTVCISTT 298
>NP_689594
          Length = 3549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TACLSTSK 9
           T+C+STS+
Sbjct: 412 TSCVSTSQ 419
>NP_065928
          Length = 4427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VTACLSTSK 9
            VT CL T+K
Sbjct: 1663 VTKCLLTAK 1671
>NP_065831
          Length = 1392

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VTACLST 7
           +TAC+ST
Sbjct: 884 LTACMST 890
>XP_005256527
          Length = 4427

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VTACLSTSK 9
            VT CL T+K
Sbjct: 1663 VTKCLLTAK 1671
>XP_006712319
          Length = 3308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TACLSTSK 9
           T+C+STS+
Sbjct: 171 TSCVSTSQ 178
>NP_001186073
          Length = 3327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TACLSTSK 9
           T+C+STS+
Sbjct: 190 TSCVSTSQ 197
>NP_001027563
          Length = 279

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VTACLSTS 8
          +T+C STS
Sbjct: 10 ITSCCSTS 17
>NP_057393
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VTACLSTS 8
          +T+C STS
Sbjct: 10 ITSCCSTS 17
>NP_000789
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  VTACLST 7
          VTACL T
Sbjct: 42 VTACLLT 48
>NP_031375
          Length = 1069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VTACLS 6
           +TACLS
Sbjct: 102 ITACLS 107
>XP_005276205
          Length = 199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  VTACLST 7
          VTACL T
Sbjct: 87 VTACLLT 93
>XP_005249593
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VTACLS 6
           +TACLS
Sbjct: 102 ITACLS 107
>XP_005277516
          Length = 199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  VTACLST 7
          VTACL T
Sbjct: 87 VTACLLT 93
>NP_001026
          Length = 4967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>NP_061027
          Length = 4599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ACLSTSK 9
          ACL T+K
Sbjct: 83 ACLGTNK 89
>NP_060501
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  CLSTSK 9
          CLSTS+
Sbjct: 23 CLSTSR 28
>NP_689414
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   VTACLST 7
           +T CLST
Sbjct: 644 LTGCLST 650
>NP_001154994
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           V++C+ST +
Sbjct: 485 VSSCMSTGR 493
>NP_009177
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           V++C+ST +
Sbjct: 573 VSSCMSTGR 581
>NP_001154993
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           V++C+ST +
Sbjct: 577 VSSCMSTGR 585
>XP_005262767
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           V++C+ST +
Sbjct: 485 VSSCMSTGR 493
>XP_006714137
          Length = 588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTACLSTSK 9
           V++C+ST +
Sbjct: 573 VSSCMSTGR 581
>XP_006711873
          Length = 4954

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711872
          Length = 2735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711871
          Length = 4906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711870
          Length = 4965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711869
          Length = 4973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711868
          Length = 4975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711867
          Length = 4977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711866
          Length = 4984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
>XP_006711865
          Length = 4985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CLSTSK 9
           CLSTS+
Sbjct: 132 CLSTSR 137
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.110    0.302 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 561,102
Number of extensions: 435
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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