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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              19     869
NP_009297                                                              19     869
NP_005392                                                              18    1482
NP_776189                                                              17    4312
NP_001123501                                                           17    4312
XP_005268474                                                           17    4312
XP_005268472                                                           17    4312
XP_005268471                                                           17    4312
NP_001155000                                                           16    7355
XP_006714497                                                           16    7355
NP_001129396                                                           16    7355
NP_056197                                                              15    9605
NP_056147                                                              15    9605
NP_001073143                                                           15    9605
NP_001977                                                              15    9605
NP_004723                                                              15    9605
XP_005256906                                                           15    9605
XP_005267560                                                           15    9605
XP_005267559                                                           15    9605
XP_005272099                                                           15    9605

>NP_005148
          Length = 1130

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSNGALRES 9
           V NGALRES
Sbjct: 668 VPNGALRES 676



>NP_009297
          Length = 1149

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSNGALRES 9
           V NGALRES
Sbjct: 687 VPNGALRES 695



>NP_005392
          Length = 1187

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   VSNGALRE 8
           VSNGALR+
Sbjct: 759 VSNGALRQ 766



>NP_776189
          Length = 303

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VSNGALRES 9
           V NG LR+S
Sbjct: 237 VQNGGLRDS 245



>NP_001123501
          Length = 303

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VSNGALRES 9
           V NG LR+S
Sbjct: 237 VQNGGLRDS 245



>XP_005268474
          Length = 348

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VSNGALRES 9
           V NG LR+S
Sbjct: 237 VQNGGLRDS 245



>XP_005268472
          Length = 349

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VSNGALRES 9
           V NG LR+S
Sbjct: 237 VQNGGLRDS 245



>XP_005268471
          Length = 349

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VSNGALRES 9
           V NG LR+S
Sbjct: 237 VQNGGLRDS 245



>NP_001155000
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VSNGALRE 8
           +SN A+RE
Sbjct: 658 ISNNAIRE 665



>XP_006714497
          Length = 274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SNGALRES 9
          SNGA +ES
Sbjct: 6  SNGASKES 13



>NP_001129396
          Length = 1590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NGALRES 9
           NG LRES
Sbjct: 496 NGQLRES 502



>NP_056197
          Length = 2610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSNGALR 7
           VS+GALR
Sbjct: 202 VSDGALR 208



>NP_056147
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NGALRES 9
           NGA+RE+
Sbjct: 183 NGAVREN 189



>NP_001073143
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  NGALRES 9
          NG+LRE+
Sbjct: 25 NGSLREA 31



>NP_001977
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  NGALRES 9
          NG+LRE+
Sbjct: 25 NGSLREA 31



>NP_004723
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GALRES 9
          GALRES
Sbjct: 66 GALRES 71



>XP_005256906
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GALRES 9
          GALRES
Sbjct: 66 GALRES 71



>XP_005267560
          Length = 2563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSNGALR 7
           VS+GALR
Sbjct: 202 VSDGALR 208



>XP_005267559
          Length = 2610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSNGALR 7
           VS+GALR
Sbjct: 202 VSDGALR 208



>XP_005272099
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NGALRES 9
           NG +RES
Sbjct: 576 NGVIRES 582



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.117    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 563,249
Number of extensions: 658
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)