Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1135
NP_005913                                                              18    1135
XP_005267046                                                           18    1135
XP_005267045                                                           18    1135
NP_006022                                                              16    7355
XP_005261186                                                           16    7355
XP_005261185                                                           16    7355
XP_005261184                                                           16    7355
XP_005261183                                                           16    7355
XP_005261182                                                           16    7355
XP_005261181                                                           16    7355
NP_002664                                                              15    9605
NP_001123554                                                           15    9605
NP_001127891                                                           15    9605
XP_006713269                                                           15    9605
XP_005271302                                                           15    9605
XP_005271301                                                           15    9605
XP_005271298                                                           15    9605
NP_001127892                                                           15    9605
NP_003494                                                              15    9605
>NP_006715
          Length = 1558

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SIAAELQF 9
            SIAAELQF
Sbjct: 1187 SIAAELQF 1194
>NP_005913
          Length = 1608

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SIAAELQF 9
            SIAAELQF
Sbjct: 1237 SIAAELQF 1244
>XP_005267046
          Length = 1554

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SIAAELQF 9
            SIAAELQF
Sbjct: 1183 SIAAELQF 1190
>XP_005267045
          Length = 1604

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    SIAAELQF 9
            SIAAELQF
Sbjct: 1233 SIAAELQF 1240
>NP_006022
          Length = 3336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 3036 MAAELQF 3042
>XP_005261186
          Length = 3139

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 2839 MAAELQF 2845
>XP_005261185
          Length = 3229

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 2929 MAAELQF 2935
>XP_005261184
          Length = 3258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 3047 MAAELQF 3053
>XP_005261183
          Length = 3268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 2968 MAAELQF 2974
>XP_005261182
          Length = 3346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 3046 MAAELQF 3052
>XP_005261181
          Length = 3347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    IAAELQF 9
            +AAELQF
Sbjct: 3047 MAAELQF 3053
>NP_002664
          Length = 2135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSIAAELQF 9
           VS++ EL+F
Sbjct: 606 VSVSVELRF 614
>NP_001123554
          Length = 2135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSIAAELQF 9
           VS++ EL+F
Sbjct: 606 VSVSVELRF 614
>NP_001127891
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSIAAELQ 8
           + IAAELQ
Sbjct: 99  IRIAAELQ 106
>XP_006713269
          Length = 2052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSIAAELQF 9
           VS++ EL+F
Sbjct: 523 VSVSVELRF 531
>XP_005271302
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSIAAELQ 8
           + IAAELQ
Sbjct: 99  IRIAAELQ 106
>XP_005271301
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSIAAELQ 8
           + IAAELQ
Sbjct: 99  IRIAAELQ 106
>XP_005271298
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSIAAELQ 8
           + IAAELQ
Sbjct: 99  IRIAAELQ 106
>NP_001127892
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSIAAELQ 8
           + IAAELQ
Sbjct: 99  IRIAAELQ 106
>NP_003494
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSIAAELQ 8
           + IAAELQ
Sbjct: 99  IRIAAELQ 106
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.132    0.314 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 282,334
Number of extensions: 218
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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