Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000542                                                              20     299
NP_937799                                                              20     299
NP_112565                                                              17    3301
NP_002931                                                              17    3301
NP_001035809                                                           17    3301
NP_001180215                                                           17    4312
NP_036369                                                              17    4312
NP_085096                                                              17    4312
NP_061092                                                              17    4312
NP_001032728                                                           17    4312
XP_006723174                                                           17    4312
XP_005247642                                                           17    4312
XP_005247641                                                           17    4312
XP_005247640                                                           17    4312
XP_005247639                                                           17    4312
NP_001191355                                                           16    5631
NP_005060                                                              16    5631
NP_002305                                                              16    5631
XP_005264359                                                           16    5631
NP_033664                                                              16    5631
NP_001073943                                                           16    5631
NP_001139280                                                           16    7355
NP_000623                                                              16    7355
NP_001979                                                              16    7355
NP_775872                                                              16    7355
NP_055401                                                              16    7355
NP_001093120                                                           16    7355
XP_006721108                                                           16    7355
NP_036230                                                              15    9605
NP_057331                                                              15    9605
NP_112203                                                              15    9605
NP_001138396                                                           15    9605
NP_001185982                                                           15    9605
NP_569733                                                              15    9605
NP_904324                                                              15    9605
NP_689763                                                              15    9605
XP_005255663                                                           15    9605
XP_005255661                                                           15    9605
XP_006719745                                                           15    9605
XP_006719744                                                           15    9605
XP_005254044                                                           15    9605
XP_006719294                                                           15    9605
XP_006714820                                                           15    9605
XP_005268437                                                           15    9605
NP_001138947                                                           15    9605
NP_001012660                                                           15    9605
>NP_000542
          Length = 213

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRSLVKPDN 9
           VRSLVKP+N
Sbjct: 166 VRSLVKPEN 174
>NP_937799
          Length = 172

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRSLVKPDN 9
           VRSLVKP+N
Sbjct: 125 VRSLVKPEN 133
>NP_112565
          Length = 123

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LVKPDN 9
          LVKPDN
Sbjct: 6  LVKPDN 11
>NP_002931
          Length = 599

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRSLVKPD 8
           +RSL+ PD
Sbjct: 259 IRSLINPD 266
>NP_001035809
          Length = 599

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRSLVKPD 8
           +RSL+ PD
Sbjct: 259 IRSLINPD 266
>NP_001180215
          Length = 234

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           +SLVKPD
Sbjct: 188 QSLVKPD 194
>NP_036369
          Length = 389

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           +SLVKPD
Sbjct: 225 QSLVKPD 231
>NP_085096
          Length = 352

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           +SLVKPD
Sbjct: 188 QSLVKPD 194
>NP_061092
          Length = 379

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 95  IRSLIKP 101
>NP_001032728
          Length = 329

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 95  IRSLIKP 101
>XP_006723174
          Length = 352

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           +SLVKPD
Sbjct: 188 QSLVKPD 194
>XP_005247642
          Length = 344

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 95  IRSLIKP 101
>XP_005247641
          Length = 345

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 95  IRSLIKP 101
>XP_005247640
          Length = 360

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 95  IRSLIKP 101
>XP_005247639
          Length = 394

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 95  IRSLIKP 101
>NP_001191355
          Length = 613

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRSLVKPD 8
           +RSL KPD
Sbjct: 461 LRSLKKPD 468
>NP_005060
          Length = 667

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RSLVKPDN 9
           R LVKP N
Sbjct: 367 RKLVKPKN 374
>NP_002305
          Length = 647

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRSLVKPD 8
           +RSL KPD
Sbjct: 495 LRSLKKPD 502
>XP_005264359
          Length = 922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VRSLVKPDN 9
           +RSL KP N
Sbjct: 835 LRSLPKPQN 843
>NP_033664
          Length = 570

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RSLVKPDN 9
           R LVKP N
Sbjct: 367 RKLVKPKN 374
>NP_001073943
          Length = 922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VRSLVKPDN 9
           +RSL KP N
Sbjct: 835 LRSLPKPQN 843
>NP_001139280
          Length = 1153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RSLVKP 7
           RSLVKP
Sbjct: 212 RSLVKP 217
>NP_000623
          Length = 1152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RSLVKP 7
           RSLVKP
Sbjct: 212 RSLVKP 217
>NP_001979
          Length = 2033

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRSLVKPD 8
           +RSLV PD
Sbjct: 196 LRSLVGPD 203
>NP_775872
          Length = 440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 395 IRSLLKP 401
>NP_055401
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VRSLVKPD 8
           +RSL++PD
Sbjct: 297 LRSLLEPD 304
>NP_001093120
          Length = 409

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSL+KP
Sbjct: 364 IRSLLKP 370
>XP_006721108
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   RSLVKP 7
           RSLVKP
Sbjct: 212 RSLVKP 217
>NP_036230
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 293 LVKPEN 298
>NP_057331
          Length = 1241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SLVKPD 8
           S+VKPD
Sbjct: 764 SMVKPD 769
>NP_112203
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 261 LVKPEN 266
>NP_001138396
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 261 LVKPEN 266
>NP_001185982
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 261 LVKPEN 266
>NP_569733
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           RS++KP+
Sbjct: 904 RSIIKPE 910
>NP_904324
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           RS++KP+
Sbjct: 904 RSIIKPE 910
>NP_689763
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 626 LVKPEN 631
>XP_005255663
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 261 LVKPEN 266
>XP_005255661
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 261 LVKPEN 266
>XP_006719745
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           RS++KP+
Sbjct: 904 RSIIKPE 910
>XP_006719744
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           RS++KP+
Sbjct: 904 RSIIKPE 910
>XP_005254044
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   RSLVKPD 8
           RS++KP+
Sbjct: 904 RSIIKPE 910
>XP_006719294
          Length = 2348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSLV+P
Sbjct: 582 IRSLVEP 588
>XP_006714820
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 626 LVKPEN 631
>XP_005268437
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LVKPDN 9
           LVKP+N
Sbjct: 632 LVKPEN 637
>NP_001138947
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRSLVKP 7
           +RSLV+P
Sbjct: 582 IRSLVEP 588
>NP_001012660
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RSLVKPDN 9
           RSL  PDN
Sbjct: 217 RSLSLPDN 224
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.133    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 614,107
Number of extensions: 717
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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