Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              19     869
NP_060733                                                              17    2528
XP_006712103                                                           17    2528
XP_006712102                                                           17    2528
NP_005581                                                              17    4312
NP_060718                                                              17    4312
NP_001240781                                                           16    5631
NP_006585                                                              16    5631
NP_001240782                                                           16    5631
NP_852607                                                              16    5631
NP_689472                                                              16    5631
XP_006721133                                                           16    5631
XP_005255502                                                           16    5631
XP_005255501                                                           16    5631
XP_005270439                                                           16    5631
XP_005270438                                                           16    5631
NP_001121773                                                           16    5631
NP_001121774                                                           16    5631
NP_065157                                                              16    5631
NP_061031                                                              16    7355
NP_057726                                                              16    7355
NP_001138985                                                           16    7355
NP_055581                                                              16    7355
NP_001273483                                                           16    7355
NP_001138987                                                           16    7355
NP_001138986                                                           16    7355
NP_004177                                                              16    7355
XP_005254495                                                           16    7355
XP_005249975                                                           16    7355
XP_006713353                                                           16    7355
XP_005265438                                                           16    7355
NP_001139241                                                           15    9605
NP_004619                                                              15    9605
NP_071881                                                              15    9605
NP_006218                                                              15    9605
NP_872617                                                              15    9605
NP_001229849                                                           15    9605
NP_588613                                                              15    9605
NP_001034486                                                           15    9605
NP_001008738                                                           15    9605
NP_001229850                                                           15    9605
XP_005260496                                                           15    9605
XP_006721561                                                           15    9605
XP_006712137                                                           15    9605
XP_005264538                                                           15    9605
XP_005264537                                                           15    9605
XP_006712136                                                           15    9605
XP_006710222                                                           15    9605
XP_006710221                                                           15    9605
XP_001717475                                                           15    9605
>NP_004251
          Length = 1208

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VRGRALRS 8
           VRGRALRS
Sbjct: 364 VRGRALRS 371
>NP_060733
          Length = 1435

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    VRGRALRST 9
            VRG+A+RS+
Sbjct: 1277 VRGKAIRSS 1285
>XP_006712103
          Length = 1175

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    VRGRALRST 9
            VRG+A+RS+
Sbjct: 1017 VRGKAIRSS 1025
>XP_006712102
          Length = 1313

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    VRGRALRST 9
            VRG+A+RS+
Sbjct: 1155 VRGKAIRSS 1163
>NP_005581
          Length = 680

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           RGRA +ST
Sbjct: 592 RGRAFKST 599
>NP_060718
          Length = 605

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  VRGRALRS 8
          VRG ALRS
Sbjct: 27 VRGSALRS 34
>NP_001240781
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           VRG ALRS
Sbjct: 101 VRGLALRS 108
>NP_006585
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           VRG ALRS
Sbjct: 101 VRGLALRS 108
>NP_001240782
          Length = 640

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1 VRGRALRS 8
         VRG ALRS
Sbjct: 2 VRGLALRS 9
>NP_852607
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VRGRALR 7
           +RGRALR
Sbjct: 391 MRGRALR 397
>NP_689472
          Length = 1910

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           RGR LRS+
Sbjct: 623 RGRTLRSS 630
>XP_006721133
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           +RGR LRS
Sbjct: 95  LRGRGLRS 102
>XP_005255502
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           +RGR LRS
Sbjct: 95  LRGRGLRS 102
>XP_005255501
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           +RGR LRS
Sbjct: 95  LRGRGLRS 102
>XP_005270439
          Length = 405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           VRG ALRS
Sbjct: 101 VRGLALRS 108
>XP_005270438
          Length = 635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           VRG ALRS
Sbjct: 101 VRGLALRS 108
>NP_001121773
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           +RGR LRS
Sbjct: 95  LRGRGLRS 102
>NP_001121774
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           +RGR LRS
Sbjct: 95  LRGRGLRS 102
>NP_065157
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRGRALRS 8
           +RGR LRS
Sbjct: 95  LRGRGLRS 102
>NP_061031
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RGRALRS 8
           RGR LRS
Sbjct: 113 RGRTLRS 119
>NP_057726
          Length = 3674

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    RGRALRST 9
            RG ALR T
Sbjct: 1829 RGHALRDT 1836
>NP_001138985
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           +GR LRST
Sbjct: 364 QGRKLRST 371
>NP_055581
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           +GR LRST
Sbjct: 364 QGRKLRST 371
>NP_001273483
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RGRALRS 8
           RGR LRS
Sbjct: 113 RGRTLRS 119
>NP_001138987
          Length = 346

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           +GR LRST
Sbjct: 296 QGRKLRST 303
>NP_001138986
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           +GR LRST
Sbjct: 384 QGRKLRST 391
>NP_004177
          Length = 785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VRGRALRST 9
           VRGR  R+T
Sbjct: 162 VRGRGSRAT 170
>XP_005254495
          Length = 3694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    RGRALRST 9
            RG ALR T
Sbjct: 1849 RGHALRDT 1856
>XP_005249975
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGRALRST 9
           +GR LRST
Sbjct: 364 QGRKLRST 371
>XP_006713353
          Length = 484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VRGRALRST 9
           VRGR  R+T
Sbjct: 162 VRGRGSRAT 170
>XP_005265438
          Length = 785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VRGRALRST 9
           VRGR  R+T
Sbjct: 162 VRGRGSRAT 170
>NP_001139241
          Length = 903

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RGRALRS 8
          RGR LRS
Sbjct: 12 RGRELRS 18
>NP_004619
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  RGRALRS 8
          RGR LRS
Sbjct: 12 RGRELRS 18
>NP_071881
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VRGRALR 7
           V GRALR
Sbjct: 115 VNGRALR 121
>NP_006218
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VRGRALRS 8
           +RG+ALR+
Sbjct: 379 LRGKALRT 386
>NP_872617
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VRGRALRS 8
           +RG+ALR+
Sbjct: 327 LRGKALRT 334
>NP_001229849
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VRGRALRS 8
           +RG+ALR+
Sbjct: 284 LRGKALRT 291
>NP_588613
          Length = 1166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRALRST 9
           G+A+RST
Sbjct: 719 GKAMRST 725
>NP_001034486
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VRGRAL 6
           VRGRAL
Sbjct: 106 VRGRAL 111
>NP_001008738
          Length = 1138

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GRALRST 9
           G+A+RST
Sbjct: 691 GKAMRST 697
>NP_001229850
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VRGRALRS 8
           +RG+ALR+
Sbjct: 291 LRGKALRT 298
>XP_005260496
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VRGRALRS 8
           +RG+ALR+
Sbjct: 399 LRGKALRT 406
>XP_006721561
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VRGRAL 6
           VRGRAL
Sbjct: 200 VRGRAL 205
>XP_006712137
          Length = 339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VRGRALR 7
           V GRALR
Sbjct: 115 VNGRALR 121
>XP_005264538
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VRGRALR 7
           V GRALR
Sbjct: 115 VNGRALR 121
>XP_005264537
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VRGRALR 7
           V GRALR
Sbjct: 115 VNGRALR 121
>XP_006712136
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VRGRALR 7
           V GRALR
Sbjct: 115 VNGRALR 121
>XP_006710222
          Length = 1793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VRGRALRST 9
           +RG  LR+T
Sbjct: 105 IRGNLLRAT 113
>XP_006710221
          Length = 1793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VRGRALRST 9
           +RG  LR+T
Sbjct: 105 IRGNLLRAT 113
>XP_001717475
          Length = 1826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VRGRALRST 9
           +RG  LR+T
Sbjct: 138 IRGNLLRAT 146
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.134    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,522
Number of extensions: 534
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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