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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1935
NP_001263689                                                           18    1935
NP_001119584                                                           18    1935
NP_001119586                                                           18    1935
NP_001263625                                                           18    1935
NP_001119590                                                           18    1935
NP_001263690                                                           18    1935
NP_001119585                                                           18    1935
NP_001263624                                                           18    1935
NP_001119588                                                           18    1935
NP_001119587                                                           18    1935
NP_001119589                                                           18    1935
NP_031392                                                              17    2528
NP_001007268                                                           17    2528
NP_001182570                                                           17    2528
XP_005246450                                                           17    2528
XP_005246449                                                           17    2528
NP_001005497                                                           17    3301
NP_001005275                                                           16    5631
NP_001001674                                                           16    5631
NP_001004758                                                           16    5631
NP_079042                                                              15    9605
NP_085135                                                              15    9605
NP_112155                                                              15    9605
NP_001036048                                                           15    9605
NP_001036050                                                           15    9605
NP_001036049                                                           15    9605
NP_001172043                                                           15    9605
XP_005258413                                                           15    9605

>NP_000537
          Length = 393

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001263689
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119584
          Length = 393

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001119586
          Length = 341

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001263625
          Length = 302

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119590
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001263690
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119585
          Length = 346

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001263624
          Length = 307

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMAICKQ 9
           VRAMAI KQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119588
          Length = 209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VRAMAICKQ 9
          VRAMAI KQ
Sbjct: 25 VRAMAIYKQ 33



>NP_001119587
          Length = 261

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VRAMAICKQ 9
          VRAMAI KQ
Sbjct: 25 VRAMAIYKQ 33



>NP_001119589
          Length = 214

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VRAMAICKQ 9
          VRAMAI KQ
Sbjct: 25 VRAMAIYKQ 33



>NP_031392
          Length = 1463

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RAMAICKQ 9
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>NP_001007268
          Length = 1324

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RAMAICKQ 9
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>NP_001182570
          Length = 1461

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RAMAICKQ 9
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>XP_005246450
          Length = 1461

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RAMAICKQ 9
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>XP_005246449
          Length = 1463

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RAMAICKQ 9
           +AM++CKQ
Sbjct: 638 KAMSLCKQ 645



>NP_001005497
          Length = 312

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RAMAICK 8
           R MAICK
Sbjct: 120 RCMAICK 126



>NP_001005275
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RAMAICK 8
           R MAICK
Sbjct: 150 RYMAICK 156



>NP_001001674
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RAMAICK 8
           R MAICK
Sbjct: 122 RYMAICK 128



>NP_001004758
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RAMAICK 8
           RA+AIC+
Sbjct: 130 RALAICR 136



>NP_079042
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   RAMAICK 8
           RA+A+C+
Sbjct: 146 RALAVCE 152



>NP_085135
          Length = 2248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    VRAMAICK 8
            ++AM +CK
Sbjct: 2217 LKAMIVCK 2224



>NP_112155
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   VRAMAICK 8
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001036048
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   VRAMAICK 8
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001036050
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   VRAMAICK 8
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001036049
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   VRAMAICK 8
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001172043
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  VRAMAICK 8
          +RA  ICK
Sbjct: 64 IRAQNICK 71



>XP_005258413
          Length = 2249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    VRAMAICK 8
            ++AM +CK
Sbjct: 2218 LKAMIVCK 2225



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.131    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 537,483
Number of extensions: 342
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)