Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     665
NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_003195                                                              17    4312
NP_037458                                                              17    4312
NP_001263271                                                           17    4312
XP_006722508                                                           17    4312
XP_005257472                                                           17    4312
XP_005257470                                                           17    4312
XP_005257469                                                           17    4312
XP_005257468                                                           17    4312
XP_005257467                                                           17    4312
NP_006717                                                              16    5631
NP_056493                                                              16    5631
NP_001186211                                                           16    5631
XP_006719869                                                           16    5631
XP_006719868                                                           16    5631
XP_005266409                                                           16    5631
XP_005266407                                                           16    5631
XP_005266403                                                           16    5631
XP_006719867                                                           16    5631
XP_006719866                                                           16    5631
XP_005266405                                                           16    5631
XP_005266404                                                           16    5631
XP_005263433                                                           16    5631
XP_005263432                                                           16    5631
XP_005263431                                                           16    5631
XP_006714492                                                           16    5631
XP_005263430                                                           16    5631
XP_005263429                                                           16    5631
NP_001191126                                                           16    5631
NP_001157871                                                           16    7355
NP_653197                                                              15    9605
NP_001275710                                                           15    9605
NP_938051                                                              15    9605
NP_938052                                                              15    9605
NP_001275711                                                           15    9605
NP_001275712                                                           15    9605
XP_006721522                                                           15    9605
XP_006720100                                                           15    9605
XP_006720099                                                           15    9605
XP_006720098                                                           15    9605
NP_653282                                                              15    9605
NP_001151733                                                           15    9605
>NP_001245210
          Length = 868

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VRALNLFQ 8
           VRALNLFQ
Sbjct: 241 VRALNLFQ 248
>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VRALNLFQ 8
           VRALNLFQ
Sbjct: 307 VRALNLFQ 314
>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VRALNLFQ 8
           VRALNLFQ
Sbjct: 307 VRALNLFQ 314
>NP_003195
          Length = 772

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VRALNLFQV 9
          VRAL+ FQV
Sbjct: 87 VRALDRFQV 95
>NP_037458
          Length = 657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRALNLFQV 9
           +R+LN FQ+
Sbjct: 487 IRSLNQFQI 495
>NP_001263271
          Length = 561

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRALNLFQV 9
           +R+LN FQ+
Sbjct: 439 IRSLNQFQI 447
>XP_006722508
          Length = 609

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRALNLFQV 9
           +R+LN FQ+
Sbjct: 487 IRSLNQFQI 495
>XP_005257472
          Length = 731

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VRALNLFQV 9
          VRAL+ FQV
Sbjct: 87 VRALDRFQV 95
>XP_005257470
          Length = 742

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VRALNLFQV 9
          VRAL+ FQV
Sbjct: 87 VRALDRFQV 95
>XP_005257469
          Length = 742

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VRALNLFQV 9
          VRAL+ FQV
Sbjct: 87 VRALDRFQV 95
>XP_005257468
          Length = 761

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VRALNLFQV 9
          VRAL+ FQV
Sbjct: 87 VRALDRFQV 95
>XP_005257467
          Length = 772

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VRALNLFQV 9
          VRAL+ FQV
Sbjct: 87 VRALDRFQV 95
>NP_006717
          Length = 2863

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2438 VRALNVF 2444
>NP_056493
          Length = 2946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2512 VRALNVF 2518
>NP_001186211
          Length = 2851

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2427 VRALNVF 2433
>XP_006719869
          Length = 2943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2509 VRALNVF 2515
>XP_006719868
          Length = 2938

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2509 VRALNVF 2515
>XP_005266409
          Length = 1430

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VRALNLF 7
           VRALN+F
Sbjct: 975 VRALNVF 981
>XP_005266407
          Length = 2641

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2186 VRALNVF 2192
>XP_005266403
          Length = 2941

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2512 VRALNVF 2518
>XP_006719867
          Length = 2961

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2506 VRALNVF 2512
>XP_006719866
          Length = 2962

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2512 VRALNVF 2518
>XP_005266405
          Length = 2964

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2509 VRALNVF 2515
>XP_005266404
          Length = 2967

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2512 VRALNVF 2518
>XP_005263433
          Length = 2832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2402 VRALNVF 2408
>XP_005263432
          Length = 2852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2427 VRALNVF 2433
>XP_005263431
          Length = 2857

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2427 VRALNVF 2433
>XP_006714492
          Length = 2867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2438 VRALNVF 2444
>XP_005263430
          Length = 2868

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2438 VRALNVF 2444
>XP_005263429
          Length = 2868

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    VRALNLF 7
            VRALN+F
Sbjct: 2438 VRALNVF 2444
>NP_001191126
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VRALNLF 7
           VRALN+F
Sbjct: 305 VRALNVF 311
>NP_001157871
          Length = 793

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RALNLFQV 9
           R LNLF++
Sbjct: 125 RRLNLFEI 132
>NP_653197
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 275 VTALNLFK 282
>NP_001275710
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 291 VTALNLFK 298
>NP_938051
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 265 VTALNLFK 272
>NP_938052
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 235 VTALNLFK 242
>NP_001275711
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VRALNLFQ 8
          V ALNLF+
Sbjct: 77 VTALNLFK 84
>NP_001275712
          Length = 276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VRALNLFQ 8
          V ALNLF+
Sbjct: 36 VTALNLFK 43
>XP_006721522
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LNLFQV 9
           LNLFQ+
Sbjct: 264 LNLFQI 269
>XP_006720100
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 235 VTALNLFK 242
>XP_006720099
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 265 VTALNLFK 272
>XP_006720098
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRALNLFQ 8
           V ALNLF+
Sbjct: 265 VTALNLFK 272
>NP_653282
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LNLFQV 9
           LNLFQ+
Sbjct: 264 LNLFQI 269
>NP_001151733
          Length = 242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LNLFQV 9
           LNLFQ+
Sbjct: 190 LNLFQI 195
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.149    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 305,140
Number of extensions: 386
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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