Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_852111                                                              21     229
NP_000136                                                              21     229
NP_000360                                                              21     229
XP_005268094                                                           21     229
NP_000224                                                              20     509
XP_005264366                                                           20     509
XP_006712078                                                           20     509
NP_073746                                                              17    3301
XP_006723397                                                           17    3301
XP_005259220                                                           17    3301
XP_006723396                                                           17    3301
XP_006723395                                                           17    3301
XP_006723394                                                           17    3301
XP_006723393                                                           17    3301
XP_006723392                                                           17    3301
NP_001181887                                                           17    4312
NP_002805                                                              17    4312
NP_736606                                                              17    4312
NP_065133                                                              17    4312
XP_005276976                                                           17    4312
NP_001136433                                                           17    4312
NP_001258349                                                           16    7355
NP_060245                                                              16    7355
NP_061141                                                              16    7355
XP_005255020                                                           16    7355
XP_006712935                                                           16    7355
XP_006712934                                                           16    7355
XP_006712933                                                           16    7355
NP_852118                                                              16    7355
NP_703146                                                              16    7355
NP_001034764                                                           16    7355
NP_006233                                                              16    7355
NP_919299                                                              15    9605
NP_001159687                                                           15    9605
NP_001028692                                                           15    9605
NP_001028690                                                           15    9605
NP_057087                                                              15    9605
NP_056425                                                              15    9605
NP_001028691                                                           15    9605
NP_001005188                                                           15    9605
NP_001265228                                                           15    9605
NP_110387                                                              15    9605
XP_005260148                                                           15    9605
XP_005259994                                                           15    9605
XP_005251505                                                           15    9605
XP_005251504                                                           15    9605
XP_006715748                                                           15    9605
NP_001004730                                                           15    9605
NP_001004483                                                           15    9605
NP_001010887                                                           15    9605
NP_795369                                                              15    9605
>NP_852111
          Length = 669

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LMCNLAFA 9
           LMCNLAFA
Sbjct: 374 LMCNLAFA 381
>NP_000136
          Length = 695

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LMCNLAFA 9
           LMCNLAFA
Sbjct: 400 LMCNLAFA 407
>NP_000360
          Length = 764

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LMCNLAFA 9
           LMCNLAFA
Sbjct: 452 LMCNLAFA 459
>XP_005268094
          Length = 764

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   LMCNLAFA 9
           LMCNLAFA
Sbjct: 452 LMCNLAFA 459
>NP_000224
          Length = 699

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LMCNLAFA 9
           LMCNL+FA
Sbjct: 397 LMCNLSFA 404
>XP_005264366
          Length = 380

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  LMCNLAFA 9
          LMCNL+FA
Sbjct: 78 LMCNLSFA 85
>XP_006712078
          Length = 389

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  LMCNLAFA 9
          LMCNL+FA
Sbjct: 87 LMCNLSFA 94
>NP_073746
          Length = 1386

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 875 VLVCELAF 882
>XP_006723397
          Length = 1327

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 816 VLVCELAF 823
>XP_005259220
          Length = 1386

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 875 VLVCELAF 882
>XP_006723396
          Length = 1386

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 875 VLVCELAF 882
>XP_006723395
          Length = 1387

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 876 VLVCELAF 883
>XP_006723394
          Length = 1406

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 895 VLVCELAF 902
>XP_006723393
          Length = 1406

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 895 VLVCELAF 902
>XP_006723392
          Length = 1406

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           VL+C LAF
Sbjct: 895 VLVCELAF 902
>NP_001181887
          Length = 433

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  CNLAFA 9
          CN+AFA
Sbjct: 53 CNIAFA 58
>NP_002805
          Length = 226

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  VLMCNLAFA 9
          +++CNLA++
Sbjct: 8  LMVCNLAYS 16
>NP_736606
          Length = 151

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  VLMCNLAFA 9
          +++CNLA++
Sbjct: 8  LMVCNLAYS 16
>NP_065133
          Length = 372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  VLMCNLA 7
          V MCNLA
Sbjct: 59 VYMCNLA 65
>XP_005276976
          Length = 535

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   CNLAFA 9
           CN+AFA
Sbjct: 155 CNIAFA 160
>NP_001136433
          Length = 372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  VLMCNLA 7
          V MCNLA
Sbjct: 59 VYMCNLA 65
>NP_001258349
          Length = 273

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  LMCNLAF 8
          LM NLAF
Sbjct: 34 LMANLAF 40
>NP_060245
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LMCNLAF 8
           LM NLAF
Sbjct: 141 LMANLAF 147
>NP_061141
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   MCNLAFA 9
           MCNLA +
Sbjct: 799 MCNLAMS 805
>XP_005255020
          Length = 799

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   MCNLAFA 9
           MCNLA +
Sbjct: 654 MCNLAMS 660
>XP_006712935
          Length = 977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   CNLAF 8
           CNLAF
Sbjct: 801 CNLAF 805
>XP_006712934
          Length = 1583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   CNLAF 8
           CNLAF
Sbjct: 697 CNLAF 701
>XP_006712933
          Length = 1687

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   CNLAF 8
           CNLAF
Sbjct: 801 CNLAF 805
>NP_852118
          Length = 1684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   CNLAF 8
           CNLAF
Sbjct: 798 CNLAF 802
>NP_703146
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  VLMCNLAF 8
          + +C+LAF
Sbjct: 61 IFLCHLAF 68
>NP_001034764
          Length = 929

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   MCNLAFA 9
           MCNLA +
Sbjct: 784 MCNLAMS 790
>NP_006233
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VLMCNLAF 8
           V +CN+AF
Sbjct: 193 VRVCNVAF 200
>NP_919299
          Length = 498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VLMCNLAF 8
          +L C+LAF
Sbjct: 18 LLTCSLAF 25
>NP_001159687
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   VLMCNLAF 8
           VL C LAF
Sbjct: 194 VLFCVLAF 201
>NP_001028692
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 190 VSICSLAFS 198
>NP_001028690
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 249 VSICSLAFS 257
>NP_057087
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 231 VSICSLAFS 239
>NP_056425
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 249 VSICSLAFS 257
>NP_001028691
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 231 VSICSLAFS 239
>NP_001005188
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3  MCNLAF 8
          +CNL+F
Sbjct: 61 LCNLSF 66
>NP_001265228
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 105 VSICSLAFS 113
>NP_110387
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   VLMCNLAF 8
           VL C LAF
Sbjct: 194 VLFCVLAF 201
>XP_005260148
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  VLMCNLAFA 9
          +L+C+++FA
Sbjct: 56 LLVCSVSFA 64
>XP_005259994
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   VLMCNLAF 8
           VL C LAF
Sbjct: 296 VLFCVLAF 303
>XP_005251505
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  VLMCNLA 7
          VLMC LA
Sbjct: 51 VLMCALA 57
>XP_005251504
          Length = 229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VLMCNLA 7
           VLMC LA
Sbjct: 100 VLMCALA 106
>XP_006715748
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLMCNLAFA 9
           V +C+LAF+
Sbjct: 105 VSICSLAFS 113
>NP_001004730
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3  MCNLAF 8
          +CNL+F
Sbjct: 63 LCNLSF 68
>NP_001004483
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3  MCNLAF 8
          +CNL+F
Sbjct: 63 LCNLSF 68
>NP_001010887
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VLMCNLA 7
           VLMC LA
Sbjct: 100 VLMCALA 106
>NP_795369
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LMCNLA 7
           L+CNLA
Sbjct: 140 LICNLA 145
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.349    0.143    0.474 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 514,090
Number of extensions: 802
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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