Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005255186                                                           18    1935
NP_777563                                                              18    1935
NP_056207                                                              17    4312
NP_000672                                                              16    5631
NP_002746                                                              16    7355
NP_001019626                                                           16    7355
NP_689838                                                              16    7355
NP_001138573                                                           16    7355
XP_005254598                                                           16    7355
XP_006718727                                                           16    7355
XP_006717976                                                           16    7355
XP_005270022                                                           16    7355
XP_003403748                                                           16    7355
NP_001129595                                                           16    7355
NP_060591                                                              16    7355
NP_619635                                                              16    7355
NP_001139784                                                           15    9605
NP_705832                                                              15    9605
NP_001269667                                                           15    9605
NP_653309                                                              15    9605
NP_001269668                                                           15    9605
NP_001269670                                                           15    9605
NP_078848                                                              15    9605
NP_001240755                                                           15    9605
NP_001269669                                                           15    9605
NP_001269671                                                           15    9605
NP_110385                                                              15    9605
XP_005262370                                                           15    9605
XP_005262369                                                           15    9605
XP_006724779                                                           15    9605
XP_006724272                                                           15    9605
XP_006724271                                                           15    9605
XP_006724270                                                           15    9605
XP_006724269                                                           15    9605
XP_006724268                                                           15    9605
XP_006724267                                                           15    9605
XP_005256920                                                           15    9605
NP_115664                                                              15    9605
NP_001011537                                                           15    9605
NP_443189                                                              15    9605
NP_000979                                                              15    9605
>XP_005255186
          Length = 254

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VLKRKARVP 9
          VLKR AR+P
Sbjct: 35 VLKRPARLP 43
>NP_777563
          Length = 245

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VLKRKARVP 9
          VLKR AR+P
Sbjct: 26 VLKRPARLP 34
>NP_056207
          Length = 325

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LKRKARVP 9
          LKR+AR P
Sbjct: 50 LKRRARCP 57
>NP_000672
          Length = 465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VLKRKARVP 9
           + KR+ RVP
Sbjct: 237 IAKRRTRVP 245
>NP_002746
          Length = 393

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VLKRKARVP 9
           VLK+  R+P
Sbjct: 154 VLKKAGRIP 162
>NP_001019626
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           ++RK RVP
Sbjct: 307 IERKVRVP 314
>NP_689838
          Length = 744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KRKARVP 9
           KR+A+VP
Sbjct: 596 KRRAKVP 602
>NP_001138573
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLKRKAR 7
           +LKRKAR
Sbjct: 206 LLKRKAR 212
>XP_005254598
          Length = 284

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VLKRKARVP 9
           VLK+  R+P
Sbjct: 154 VLKKAGRIP 162
>XP_006718727
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VLKRKAR 7
           +LKRKAR
Sbjct: 206 LLKRKAR 212
>XP_006717976
          Length = 1147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VLKRKARVP 9
            VLK+KA  P
Sbjct: 1061 VLKKKAEQP 1069
>XP_005270022
          Length = 1172

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VLKRKARVP 9
            VLK+KA  P
Sbjct: 1060 VLKKKAEQP 1068
>XP_003403748
          Length = 997

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VLKRKARVP 9
          +L+R+AR P
Sbjct: 84 LLRREARAP 92
>NP_001129595
          Length = 1186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VLKRKARVP 9
            VLK+KA  P
Sbjct: 1061 VLKKKAEQP 1069
>NP_060591
          Length = 1173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    VLKRKARVP 9
            VLK+KA  P
Sbjct: 1061 VLKKKAEQP 1069
>NP_619635
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLKRKARVP 9
           +L+R+AR P
Sbjct: 130 LLRREARAP 138
>NP_001139784
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LKRKAR 7
          LKRKAR
Sbjct: 64 LKRKAR 69
>NP_705832
          Length = 938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LKRKAR 7
          LKRKAR
Sbjct: 64 LKRKAR 69
>NP_001269667
          Length = 750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 341 VKRRVRIP 348
>NP_653309
          Length = 1434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           LK+K +VP
Sbjct: 329 LKKKMKVP 336
>NP_001269668
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 506 VKRRVRIP 513
>NP_001269670
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 249 VKRRVRIP 256
>NP_078848
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  VLKRKARVP 9
          V KRK  VP
Sbjct: 26 VTKRKLEVP 34
>NP_001240755
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  VLKRKARVP 9
          V KRK  VP
Sbjct: 26 VTKRKLEVP 34
>NP_001269669
          Length = 549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 140 VKRRVRIP 147
>NP_001269671
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  LKRKARVP 9
          +KR+ R+P
Sbjct: 51 VKRRVRIP 58
>NP_110385
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 507 VKRRVRIP 514
>XP_005262370
          Length = 664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VLKRKARVP 9
           V KRK R P
Sbjct: 456 VEKRKTRTP 464
>XP_005262369
          Length = 664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VLKRKARVP 9
           V KRK R P
Sbjct: 456 VEKRKTRTP 464
>XP_006724779
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VLKRKARVP 9
           V KRK R P
Sbjct: 483 VEKRKTRTP 491
>XP_006724272
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 141 VKRRVRIP 148
>XP_006724271
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  LKRKARVP 9
          +KR+ R+P
Sbjct: 52 VKRRVRIP 59
>XP_006724270
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 141 VKRRVRIP 148
>XP_006724269
          Length = 639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 230 VKRRVRIP 237
>XP_006724268
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 333 VKRRVRIP 340
>XP_006724267
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   LKRKARVP 9
           +KR+ R+P
Sbjct: 334 VKRRVRIP 341
>XP_005256920
          Length = 943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LKRKAR 7
          LKRKAR
Sbjct: 64 LKRKAR 69
>NP_115664
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKRKARV 8
           VLKRKA +
Sbjct: 136 VLKRKANL 143
>NP_001011537
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VLKRKARV 8
           VLKRKA +
Sbjct: 110 VLKRKANL 117
>NP_443189
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   VLKRKARVP 9
           V KRK R P
Sbjct: 483 VEKRKTRTP 491
>NP_000979
          Length = 136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LKRKAR 7
           LKRKAR
Sbjct: 106 LKRKAR 111
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.139    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 459,374
Number of extensions: 797
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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