Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              17    2528
XP_006714104                                                           17    2528
XP_005265800                                                           17    2528
XP_006714103                                                           17    2528
XP_006714102                                                           17    2528
NP_005520                                                              17    3301
NP_005038                                                              17    3301
NP_001257356                                                           17    3301
XP_006710661                                                           17    3301
XP_005245920                                                           17    3301
XP_006710660                                                           17    3301
XP_006710659                                                           17    3301
XP_006710658                                                           17    3301
XP_006710657                                                           17    3301
NP_004632                                                              17    4312
NP_001182555                                                           17    4312
NP_003043                                                              17    4312
NP_001161051                                                           17    4312
XP_005252669                                                           17    4312
XP_005252668                                                           17    4312
XP_006717572                                                           17    4312
XP_005252667                                                           17    4312
XP_005252666                                                           17    4312
XP_005252665                                                           17    4312
XP_005252663                                                           17    4312
XP_005252662                                                           17    4312
XP_005266032                                                           17    4312
XP_005266031                                                           17    4312
NP_000480                                                              16    7355
NP_612114                                                              16    7355
NP_064522                                                              16    7355
XP_006724731                                                           16    7355
XP_006724730                                                           16    7355
XP_005262214                                                           16    7355
XP_005262213                                                           16    7355
XP_006724729                                                           16    7355
XP_005262212                                                           16    7355
XP_005262211                                                           16    7355
XP_005262210                                                           16    7355
XP_005272685                                                           16    7355
NP_115989                                                              16    7355
NP_002995                                                              15    9605
NP_001032961                                                           15    9605
NP_079199                                                              15    9605
NP_057400                                                              15    9605
NP_001177773                                                           15    9605
XP_005256449                                                           15    9605
XP_006712656                                                           15    9605
XP_005264025                                                           15    9605
XP_006712655                                                           15    9605
NP_777567                                                              15    9605
>NP_006197
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  VLEVSSAS 8
          VLEVSSAS
Sbjct: 84 VLEVSSAS 91
>XP_006714104
          Length = 832

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VLEVSSAS 8
           VLEVSSAS
Sbjct: 109 VLEVSSAS 116
>XP_005265800
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  VLEVSSAS 8
          VLEVSSAS
Sbjct: 84 VLEVSSAS 91
>XP_006714103
          Length = 1111

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VLEVSSAS 8
           VLEVSSAS
Sbjct: 106 VLEVSSAS 113
>XP_006714102
          Length = 1114

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   VLEVSSAS 8
           VLEVSSAS
Sbjct: 109 VLEVSSAS 116
>NP_005520
          Length = 4391

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3169 VLQISSAKP 3177
>NP_005038
          Length = 504

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLEVSSASP 9
           ++E+SS SP
Sbjct: 189 IIEISSVSP 197
>NP_001257356
          Length = 461

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VLEVSSASP 9
           ++E+SS SP
Sbjct: 146 IIEISSVSP 154
>XP_006710661
          Length = 4057

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3352 VLQISSAKP 3360
>XP_005245920
          Length = 4392

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3170 VLQISSAKP 3178
>XP_006710660
          Length = 4397

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3169 VLQISSAKP 3177
>XP_006710659
          Length = 4556

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3328 VLQISSAKP 3336
>XP_006710658
          Length = 4563

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3335 VLQISSAKP 3343
>XP_006710657
          Length = 4579

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VLEVSSASP 9
            VL++SSA P
Sbjct: 3351 VLQISSAKP 3359
>NP_004632
          Length = 1027

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>NP_001182555
          Length = 1068

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>NP_003043
          Length = 639

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VLEVSSASP 9
           +LEVSSA P
Sbjct: 178 LLEVSSAIP 186
>NP_001161051
          Length = 340

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VLEVSSASP 9
           +LEVSSA P
Sbjct: 178 LLEVSSAIP 186
>XP_005252669
          Length = 584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>XP_005252668
          Length = 839

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 248 LSVSSASP 255
>XP_006717572
          Length = 1003

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>XP_005252667
          Length = 1052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 461 LSVSSASP 468
>XP_005252666
          Length = 1057

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>XP_005252665
          Length = 1068

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>XP_005252663
          Length = 1084

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>XP_005252662
          Length = 1084

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           L VSSASP
Sbjct: 493 LSVSSASP 500
>XP_005266032
          Length = 583

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VLEVSSASP 9
           +LEVSSA P
Sbjct: 178 LLEVSSAIP 186
>XP_005266031
          Length = 639

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VLEVSSASP 9
           +LEVSSA P
Sbjct: 178 LLEVSSAIP 186
>NP_000480
          Length = 2492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1520 VIEIEDASP 1528
>NP_612114
          Length = 2454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1482 VIEIEDASP 1490
>NP_064522
          Length = 841

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LEVSSAS
Sbjct: 732 LEVSSAS 738
>XP_006724731
          Length = 1871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1520 VIEIEDASP 1528
>XP_006724730
          Length = 2399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1427 VIEIEDASP 1435
>XP_005262214
          Length = 2424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1452 VIEIEDASP 1460
>XP_005262213
          Length = 2437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1465 VIEIEDASP 1473
>XP_006724729
          Length = 2453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1481 VIEIEDASP 1489
>XP_005262212
          Length = 2453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1481 VIEIEDASP 1489
>XP_005262211
          Length = 2463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1491 VIEIEDASP 1499
>XP_005262210
          Length = 2491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VLEVSSASP 9
            V+E+  ASP
Sbjct: 1519 VIEIEDASP 1527
>XP_005272685
          Length = 815

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LEVSSAS
Sbjct: 706 LEVSSAS 712
>NP_115989
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LEVSSASP 9
           +E+SSA P
Sbjct: 188 MEISSAEP 195
>NP_002995
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LEVSSASP 9
           LEVS A P
Sbjct: 131 LEVSGAEP 138
>NP_001032961
          Length = 1250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LE+SSAS
Sbjct: 878 LEISSAS 884
>NP_079199
          Length = 1887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    LEVSSASP 9
            LEV S SP
Sbjct: 1721 LEVKSGSP 1728
>NP_057400
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LE+SSAS
Sbjct: 879 LEISSAS 885
>NP_001177773
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EVSSASP 9
           EVS ASP
Sbjct: 200 EVSKASP 206
>XP_005256449
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LEVSSASP 9
           LEVS A P
Sbjct: 131 LEVSGAEP 138
>XP_006712656
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LE+SSAS
Sbjct: 389 LEISSAS 395
>XP_005264025
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LE+SSAS
Sbjct: 809 LEISSAS 815
>XP_006712655
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LEVSSAS 8
           LE+SSAS
Sbjct: 879 LEISSAS 885
>NP_777567
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EVSSASP 9
           EVSS SP
Sbjct: 221 EVSSVSP 227
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.109    0.254 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 426,427
Number of extensions: 886
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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