Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              19     665
XP_006713721                                                           19     665
NP_001135780                                                           17    2528
NP_005259                                                              17    3301
XP_005270808                                                           17    3301
NP_001010853                                                           17    4312
XP_005248719                                                           17    4312
XP_005248718                                                           17    4312
NP_060426                                                              17    4312
NP_085153                                                              16    5631
XP_005246937                                                           16    5631
XP_005246936                                                           16    5631
XP_005246935                                                           16    5631
XP_006712846                                                           16    5631
XP_005260474                                                           16    7355
NP_057088                                                              16    7355
NP_667339                                                              16    7355
NP_065681                                                              15    9605
NP_066124                                                              15    9605
NP_001766                                                              15    9605
NP_000365                                                              15    9605
NP_001010870                                                           15    9605
NP_001161831                                                           15    9605
NP_001275680                                                           15    9605
NP_001275679                                                           15    9605
NP_110397                                                              15    9605
XP_006726414                                                           15    9605
XP_006718986                                                           15    9605
XP_006718985                                                           15    9605
XP_006717999                                                           15    9605
XP_006712839                                                           15    9605
XP_006711762                                                           15    9605
XP_005271227                                                           15    9605
XP_005271226                                                           15    9605
NP_079423                                                              15    9605
>NP_006209
          Length = 1068

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VISKRAQEC 9
           VISK AQEC
Sbjct: 963 VISKGAQEC 971
>XP_006713721
          Length = 1068

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VISKRAQEC 9
           VISK AQEC
Sbjct: 963 VISKGAQEC 971
>NP_001135780
          Length = 2156

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VISKRAQEC 9
            VISKR++ C
Sbjct: 1374 VISKRSKNC 1382
>NP_005259
          Length = 273

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           ++SKR  EC
Sbjct: 208 LVSKRCHEC 216
>XP_005270808
          Length = 273

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           ++SKR  EC
Sbjct: 208 LVSKRCHEC 216
>NP_001010853
          Length = 436

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VISKRAQEC 9
           V++K+A++C
Sbjct: 285 VLTKKAEDC 293
>XP_005248719
          Length = 250

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VISKRAQEC 9
           V++K+A++C
Sbjct: 99  VLTKKAEDC 107
>XP_005248718
          Length = 250

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VISKRAQEC 9
           V++K+A++C
Sbjct: 99  VLTKKAEDC 107
>NP_060426
          Length = 448

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           V SKRAQ C
Sbjct: 93  VPSKRAQGC 101
>NP_085153
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SKRAQEC 9
           SK+A+EC
Sbjct: 143 SKKAKEC 149
>XP_005246937
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SKRAQEC 9
          SK+A+EC
Sbjct: 25 SKKAKEC 31
>XP_005246936
          Length = 353

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SKRAQEC 9
          SK+A+EC
Sbjct: 68 SKKAKEC 74
>XP_005246935
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SKRAQEC 9
           SK+A+EC
Sbjct: 143 SKKAKEC 149
>XP_006712846
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SKRAQEC 9
           SK+A+EC
Sbjct: 143 SKKAKEC 149
>XP_005260474
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISKRAQEC 9
           +SKR +EC
Sbjct: 269 LSKRNREC 276
>NP_057088
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISKRAQEC 9
           +SKR +EC
Sbjct: 269 LSKRNREC 276
>NP_667339
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           V+SK A EC
Sbjct: 191 VLSKDAGEC 199
>NP_065681
          Length = 1072

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISKRAQEC 9
           +SKR  EC
Sbjct: 521 VSKRRLEC 528
>NP_066124
          Length = 1114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISKRAQEC 9
           +SKR  EC
Sbjct: 521 VSKRRLEC 528
>NP_001766
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ISKRAQEC 9
          +S+RAQ C
Sbjct: 18 LSRRAQLC 25
>NP_000365
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           V  K+A+EC
Sbjct: 286 VAPKKAREC 294
>NP_001010870
          Length = 2096

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VISKRAQE 8
          V+S++AQE
Sbjct: 86 VVSRQAQE 93
>NP_001161831
          Length = 2066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  VISKRAQE 8
          V+S++AQE
Sbjct: 86 VVSRQAQE 93
>NP_001275680
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S R  EC
Sbjct: 192 VVSLRCSEC 200
>NP_001275679
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S R  EC
Sbjct: 157 VVSLRCSEC 165
>NP_110397
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S R  EC
Sbjct: 201 VVSLRCSEC 209
>XP_006726414
          Length = 157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S   QEC
Sbjct: 147 VVSAATQEC 155
>XP_006718986
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S R  EC
Sbjct: 192 VVSLRCSEC 200
>XP_006718985
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S R  EC
Sbjct: 201 VVSLRCSEC 209
>XP_006717999
          Length = 1207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ISKRAQEC 9
           +SKR  EC
Sbjct: 614 VSKRRLEC 621
>XP_006712839
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VISKRAQEC 9
          VISKR+  C
Sbjct: 85 VISKRSGYC 93
>XP_006711762
          Length = 157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VISKRAQEC 9
           V+S   QEC
Sbjct: 147 VVSAATQEC 155
>XP_005271227
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           V  K+A+EC
Sbjct: 214 VAPKKAREC 222
>XP_005271226
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           V  K+A+EC
Sbjct: 214 VAPKKAREC 222
>NP_079423
          Length = 238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VISKRAQEC 9
           VISKR+  C
Sbjct: 113 VISKRSGYC 121
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.125    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 459,253
Number of extensions: 718
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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