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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              21     175
XP_005271621                                                           21     175
XP_006718906                                                           21     175
XP_005271619                                                           21     175
XP_005271618                                                           21     175
NP_055736                                                              17    2528
NP_001008701                                                           17    2528
XP_005259876                                                           17    2528
XP_006722752                                                           17    2528
XP_005259875                                                           17    2528
XP_006722751                                                           17    2528
NP_071933                                                              17    3301
XP_006716233                                                           17    4312
NP_443085                                                              17    4312
NP_000375                                                              16    5631
NP_060918                                                              16    5631
NP_005903                                                              16    7355
NP_005786                                                              16    7355
NP_001258680                                                           16    7355
XP_005247225                                                           16    7355
XP_005247224                                                           16    7355
XP_005246291                                                           16    7355
XP_005246289                                                           16    7355
XP_005246288                                                           16    7355
NP_997218                                                              16    7355
NP_001029                                                              15    9605
NP_001153048                                                           15    9605
NP_001153047                                                           15    9605
NP_055445                                                              15    9605
XP_005255053                                                           15    9605
XP_005253552                                                           15    9605
XP_005253550                                                           15    9605

>NP_000042
          Length = 3056

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           +HMYLMLLK
Sbjct: 944 LHMYLMLLK 952



>XP_005271621
          Length = 2708

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           +HMYLMLLK
Sbjct: 596 LHMYLMLLK 604



>XP_006718906
          Length = 3056

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           +HMYLMLLK
Sbjct: 944 LHMYLMLLK 952



>XP_005271619
          Length = 3056

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           +HMYLMLLK
Sbjct: 944 LHMYLMLLK 952



>XP_005271618
          Length = 3056

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           +HMYLMLLK
Sbjct: 944 LHMYLMLLK 952



>NP_055736
          Length = 1469

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           VH+YL+L++
Sbjct: 941 VHLYLLLVE 949



>NP_001008701
          Length = 1474

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           VH+YL+L++
Sbjct: 946 VHLYLLLVE 954



>XP_005259876
          Length = 1498

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           VH+YL+L++
Sbjct: 946 VHLYLLLVE 954



>XP_006722752
          Length = 1462

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           VH+YL+L++
Sbjct: 910 VHLYLLLVE 918



>XP_005259875
          Length = 1493

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           VH+YL+L++
Sbjct: 941 VHLYLLLVE 949



>XP_006722751
          Length = 1498

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VHMYLMLLK 9
           VH+YL+L++
Sbjct: 946 VHLYLLLVE 954



>NP_071933
          Length = 314

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VHMYLML 7
           VHMYL++
Sbjct: 286 VHMYLLI 292



>XP_006716233
          Length = 542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMYLMLLK 9
           VHM L+L+K
Sbjct: 321 VHMDLLLMK 329



>NP_443085
          Length = 626

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMYLMLLK 9
           VHM L+L+K
Sbjct: 321 VHMDLLLMK 329



>NP_000375
          Length = 4563

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    VHMYLMLLK 9
            +H Y+M L+
Sbjct: 4377 IHQYIMALR 4385



>NP_060918
          Length = 1230

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HMYLMLLK 9
           H YL LLK
Sbjct: 396 HAYLSLLK 403



>NP_005903
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VHMYLM 6
           VHM+LM
Sbjct: 213 VHMFLM 218



>NP_005786
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VHMYLM 6
           +H+YLM
Sbjct: 218 IHLYLM 223



>NP_001258680
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VHMYLM 6
           +H+YLM
Sbjct: 218 IHLYLM 223



>XP_005247225
          Length = 623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VHMYLMLLK 9
           VH +L L+K
Sbjct: 174 VHQFLTLIK 182



>XP_005247224
          Length = 623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VHMYLMLLK 9
           VH +L L+K
Sbjct: 174 VHQFLTLIK 182



>XP_005246291
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VHMYLM 6
           +H+YLM
Sbjct: 229 IHLYLM 234



>XP_005246289
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VHMYLM 6
           +H+YLM
Sbjct: 229 IHLYLM 234



>XP_005246288
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VHMYLM 6
           +H+YLM
Sbjct: 229 IHLYLM 234



>NP_997218
          Length = 623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VHMYLMLLK 9
           VH +L L+K
Sbjct: 174 VHQFLTLIK 182



>NP_001029
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMYLMLLK 9
           V M+LMLL+
Sbjct: 578 VIMFLMLLR 586



>NP_001153048
          Length = 728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMYLMLLK 9
           V M+LMLL+
Sbjct: 637 VIMFLMLLR 645



>NP_001153047
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMYLMLLK 9
           V M+LMLL+
Sbjct: 601 VIMFLMLLR 609



>NP_055445
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VHMYLMLLK 9
           VH+ L LLK
Sbjct: 807 VHLTLALLK 815



>XP_005255053
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VHMYLMLLK 9
           VH+ L LLK
Sbjct: 807 VHLTLALLK 815



>XP_005253552
          Length = 345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VHMYLML 7
           +H+YL+L
Sbjct: 136 LHLYLLL 142



>XP_005253550
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VHMYLML 7
           +H+YL+L
Sbjct: 136 LHLYLLL 142



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.151    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,149
Number of extensions: 515
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)