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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1935
XP_005271621                                                           18    1935
XP_006718907                                                           18    1935
XP_006718906                                                           18    1935
XP_005271619                                                           18    1935
XP_005271618                                                           18    1935
NP_001129735                                                           17    2528
XP_006722712                                                           17    2528
NP_065103                                                              16    7355
NP_057006                                                              15    9605
NP_001123237                                                           15    9605
NP_001129628                                                           15    9605
NP_055725                                                              15    9605
XP_006724887                                                           15    9605

>NP_000042
          Length = 3056

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VHMLAAESI 9
            V MLAAESI
Sbjct: 1085 VRMLAAESI 1093



>XP_005271621
          Length = 2708

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VHMLAAESI 9
           V MLAAESI
Sbjct: 737 VRMLAAESI 745



>XP_006718907
          Length = 3050

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VHMLAAESI 9
            V MLAAESI
Sbjct: 1079 VRMLAAESI 1087



>XP_006718906
          Length = 3056

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VHMLAAESI 9
            V MLAAESI
Sbjct: 1085 VRMLAAESI 1093



>XP_005271619
          Length = 3056

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VHMLAAESI 9
            V MLAAESI
Sbjct: 1085 VRMLAAESI 1093



>XP_005271618
          Length = 3056

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VHMLAAESI 9
            V MLAAESI
Sbjct: 1085 VRMLAAESI 1093



>NP_001129735
          Length = 421

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMLAAESI 9
           VH+LAAE +
Sbjct: 324 VHLLAAEGL 332



>XP_006722712
          Length = 446

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VHMLAAESI 9
           VH+LAAE +
Sbjct: 324 VHLLAAEGL 332



>NP_065103
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VHMLAA 6
           VHMLAA
Sbjct: 351 VHMLAA 356



>NP_057006
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMLAAESI 9
           H+LAAE +
Sbjct: 247 HILAAEQL 254



>NP_001123237
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMLAAESI 9
           H+LAAE +
Sbjct: 247 HILAAEQL 254



>NP_001129628
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VHMLAAESI 9
           +H LAAE++
Sbjct: 492 LHSLAAEAL 500



>NP_055725
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VHMLAAESI 9
           +H LAAE++
Sbjct: 492 LHSLAAEAL 500



>XP_006724887
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HMLAAESI 9
           H+LAAE +
Sbjct: 247 HILAAEQL 254



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.125    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,243
Number of extensions: 367
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)