Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              19     665
XP_005271879                                                           17    2528
NP_000693                                                              17    4312
NP_036270                                                              17    4312
NP_060862                                                              17    4312
XP_005263172                                                           17    4312
NP_001074003                                                           16    5631
XP_005254452                                                           16    5631
XP_005254451                                                           16    5631
NP_056342                                                              16    7355
NP_001096065                                                           16    7355
NP_001096064                                                           16    7355
NP_065943                                                              16    7355
XP_005250192                                                           16    7355
XP_005250189                                                           16    7355
XP_005250188                                                           16    7355
XP_006715909                                                           16    7355
NP_001253989                                                           15    9605
NP_001253990                                                           15    9605
NP_001253988                                                           15    9605
NP_055563                                                              15    9605
NP_001269461                                                           15    9605
NP_056154                                                              15    9605
NP_001269460                                                           15    9605
NP_056042                                                              15    9605
NP_598368                                                              15    9605
NP_001092108                                                           15    9605
NP_115548                                                              15    9605
NP_852107                                                              15    9605
NP_001010844                                                           15    9605
XP_006724046                                                           15    9605
XP_006724045                                                           15    9605
XP_006724044                                                           15    9605
XP_006723822                                                           15    9605
XP_005249939                                                           15    9605
XP_006715854                                                           15    9605
XP_006715853                                                           15    9605
XP_006715400                                                           15    9605
XP_005248711                                                           15    9605
XP_006715399                                                           15    9605
XP_006712217                                                           15    9605
NP_443199                                                              15    9605
>NP_004295
          Length = 1620

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSVRKLRR
Sbjct: 130 GSVRKLRR 137
>XP_005271879
          Length = 277

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  VGSVRKLRR 9
          VGS+RK RR
Sbjct: 16 VGSLRKQRR 24
>NP_000693
          Length = 1020

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GSVRKLR 8
           GSVRK+R
Sbjct: 463 GSVRKMR 469
>NP_036270
          Length = 560

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  VGSVRKL 7
          VGS+RKL
Sbjct: 53 VGSIRKL 59
>NP_060862
          Length = 2104

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VGSVRKLRR 9
            VGSVRK+ +
Sbjct: 1719 VGSVRKIAK 1727
>XP_005263172
          Length = 2104

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VGSVRKLRR 9
            VGSVRK+ +
Sbjct: 1719 VGSVRKIAK 1727
>NP_001074003
          Length = 2214

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VGSVRKLRR 9
           +G++RKLR+
Sbjct: 178 LGALRKLRK 186
>XP_005254452
          Length = 2188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VGSVRKLRR 9
           +G++RKLR+
Sbjct: 178 LGALRKLRK 186
>XP_005254451
          Length = 2214

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VGSVRKLRR 9
           +G++RKLR+
Sbjct: 178 LGALRKLRK 186
>NP_056342
          Length = 808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VGSVRKLR 8
           +GSV KLR
Sbjct: 564 LGSVEKLR 571
>NP_001096065
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>NP_001096064
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>NP_065943
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>XP_005250192
          Length = 568

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>XP_005250189
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>XP_005250188
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>XP_006715909
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GSV++LR+
Sbjct: 372 GSVKRLRK 379
>NP_001253989
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSVRKLRR 9
           GS+RK+ R
Sbjct: 365 GSIRKMLR 372
>NP_001253990
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSVRKLRR 9
           GS+RK+ R
Sbjct: 370 GSIRKMLR 377
>NP_001253988
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSVRKLRR 9
           GS+RK+ R
Sbjct: 378 GSIRKMLR 385
>NP_055563
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSVRKLRR 9
           GS+RK+ R
Sbjct: 390 GSIRKMLR 397
>NP_001269461
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VGSVRKLRR 9
          +GS+RK R+
Sbjct: 9  LGSLRKARK 17
>NP_056154
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VGSVRKLRR 9
          +GS+RK R+
Sbjct: 9  LGSLRKARK 17
>NP_001269460
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VGSVRKLRR 9
          +GS+RK R+
Sbjct: 9  LGSLRKARK 17
>NP_056042
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GSVRKLR 8
           G+VRK+R
Sbjct: 311 GAVRKIR 317
>NP_598368
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GSVRKLR 8
           G+VRK+R
Sbjct: 311 GAVRKIR 317
>NP_001092108
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   GSVRKLRR 9
           GS++KLR+
Sbjct: 635 GSLKKLRQ 642
>NP_115548
          Length = 1316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VGSVRKLRR 9
           +GS+RK+ R
Sbjct: 942 IGSLRKVDR 950
>NP_852107
          Length = 1102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VGSVRKLRR 9
          +GS+RK R+
Sbjct: 9  LGSLRKARK 17
>NP_001010844
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GSVRKLRR 9
           GSV  LRR
Sbjct: 165 GSVENLRR 172
>XP_006724046
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GSVRKLR 8
           G+VRK+R
Sbjct: 186 GAVRKIR 192
>XP_006724045
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GSVRKLR 8
           G+VRK+R
Sbjct: 203 GAVRKIR 209
>XP_006724044
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GSVRKLR 8
           G+VRK+R
Sbjct: 331 GAVRKIR 337
>XP_006723822
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VGSVRKLRR 9
          +GS+RK R+
Sbjct: 18 LGSLRKARK 26
>XP_005249939
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VGSVRKLRR 9
           +GS+RK+ R
Sbjct: 942 IGSLRKVDR 950
>XP_006715854
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VGSVRKLRR 9
           +GS+RK+ R
Sbjct: 942 IGSLRKVDR 950
>XP_006715853
          Length = 1327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VGSVRKLRR 9
           +GS+RK+ R
Sbjct: 942 IGSLRKVDR 950
>XP_006715400
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  GSVRKLRR 9
          GSV  LRR
Sbjct: 42 GSVENLRR 49
>XP_005248711
          Length = 192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  GSVRKLRR 9
          GSV  LRR
Sbjct: 78 GSVENLRR 85
>XP_006715399
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   GSVRKLRR 9
           GSV  LRR
Sbjct: 165 GSVENLRR 172
>XP_006712217
          Length = 256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GSVRKLRR 9
           GS+RK+ R
Sbjct: 176 GSIRKMLR 183
>NP_443199
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VGSVRKLR 8
           VGS+RK R
Sbjct: 317 VGSLRKKR 324
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.144    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 468,895
Number of extensions: 744
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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