Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              18    1482
NP_001627                                                              17    2528
NP_001143                                                              17    3301
NP_001275716                                                           17    3301
NP_001275715                                                           17    3301
NP_001275717                                                           17    3301
NP_001275718                                                           17    3301
NP_001076437                                                           17    3301
NP_001028723                                                           17    3301
XP_005256523                                                           17    3301
XP_005256520                                                           17    3301
XP_005256519                                                           17    3301
XP_006721515                                                           17    3301
XP_005256518                                                           17    3301
NP_037518                                                              17    4312
NP_998816                                                              17    4312
NP_001142                                                              17    4312
NP_001740                                                              17    4312
XP_005259353                                                           17    4312
XP_005259352                                                           17    4312
NP_112581                                                              17    4312
NP_001003962                                                           17    4312
NP_061729                                                              17    4312
NP_113685                                                              17    4312
NP_001615                                                              16    5631
XP_005266896                                                           16    5631
XP_006714613                                                           16    7355
XP_006714612                                                           16    7355
XP_006714611                                                           16    7355
XP_006714610                                                           16    7355
XP_006714609                                                           16    7355
XP_006714608                                                           16    7355
XP_005271959                                                           16    7355
XP_006714606                                                           16    7355
NP_055529                                                              15    9605
XP_005255783                                                           15    9605
XP_005255782                                                           15    9605
XP_006721054                                                           15    9605
XP_006721053                                                           15    9605
XP_006721052                                                           15    9605
NP_937792                                                              15    9605
>NP_005350
          Length = 552

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VGGIASAIS 9
           VGGIA AIS
Sbjct: 475 VGGIAPAIS 483
>NP_001627
          Length = 298

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  GGIASAIS 9
          GGIA+AIS
Sbjct: 15 GGIAAAIS 22
>NP_001143
          Length = 298

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GGIASAIS 9
          GG+A+AIS
Sbjct: 15 GGVAAAIS 22
>NP_001275716
          Length = 462

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 365 VGGLASAL 372
>NP_001275715
          Length = 483

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 357 VGGLASAL 364
>NP_001275717
          Length = 389

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 312 VGGLASAL 319
>NP_001275718
          Length = 388

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 262 VGGLASAL 269
>NP_001076437
          Length = 507

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 410 VGGLASAL 417
>NP_001028723
          Length = 457

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 360 VGGLASAL 367
>XP_005256523
          Length = 438

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 312 VGGLASAL 319
>XP_005256520
          Length = 487

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 410 VGGLASAL 417
>XP_005256519
          Length = 491

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 365 VGGLASAL 372
>XP_006721515
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 377 VGGLASAL 384
>XP_005256518
          Length = 536

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   VGGIASAI 8
           VGG+ASA+
Sbjct: 410 VGGLASAL 417
>NP_037518
          Length = 477

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GGIA A+S
Sbjct: 201 GGIAGAVS 208
>NP_998816
          Length = 458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GGIA A+S
Sbjct: 182 GGIAGAVS 189
>NP_001142
          Length = 298

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  GGIASAIS 9
          GG+A+A+S
Sbjct: 15 GGVAAAVS 22
>NP_001740
          Length = 268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VGGIASAIS 9
          +GG+ SAIS
Sbjct: 62 LGGVISAIS 70
>XP_005259353
          Length = 322

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VGGIASAIS 9
          +GG+ SAIS
Sbjct: 62 LGGVISAIS 70
>XP_005259352
          Length = 322

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VGGIASAIS 9
          +GG+ SAIS
Sbjct: 62 LGGVISAIS 70
>NP_112581
          Length = 315

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  GGIASAIS 9
          GG+A+A+S
Sbjct: 27 GGVAAAVS 34
>NP_001003962
          Length = 268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VGGIASAIS 9
          +GG+ SAIS
Sbjct: 62 LGGVISAIS 70
>NP_061729
          Length = 950

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGGIASAIS 9
           VGGI  A+S
Sbjct: 570 VGGIGGAVS 578
>NP_113685
          Length = 824

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VGGIASAIS 9
           VGGI  A+S
Sbjct: 570 VGGIGGAVS 578
>NP_001615
          Length = 1723

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  GGIASAIS 9
          GG+ASA S
Sbjct: 68 GGVASAAS 75
>XP_005266896
          Length = 2131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+ASA S
Sbjct: 476 GGVASAAS 483
>XP_006714613
          Length = 276

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_006714612
          Length = 280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_006714611
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_006714610
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_006714609
          Length = 311

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_006714608
          Length = 312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_005271959
          Length = 315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>XP_006714606
          Length = 361

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GGIASAIS 9
           GG+A AIS
Sbjct: 223 GGMAGAIS 230
>NP_055529
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGGIAS 6
           VGGIAS
Sbjct: 569 VGGIAS 574
>XP_005255783
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGGIAS 6
           VGGIAS
Sbjct: 569 VGGIAS 574
>XP_005255782
          Length = 807

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGGIAS 6
           VGGIAS
Sbjct: 569 VGGIAS 574
>XP_006721054
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGGIAS 6
           VGGIAS
Sbjct: 569 VGGIAS 574
>XP_006721053
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGGIAS 6
           VGGIAS
Sbjct: 569 VGGIAS 574
>XP_006721052
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   VGGIAS 6
           VGGIAS
Sbjct: 569 VGGIAS 574
>NP_937792
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VGGIASAI 8
          + GIASA+
Sbjct: 86 IAGIASAV 93
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.130    0.325 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 326,739
Number of extensions: 483
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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