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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              16    7355
NP_061857                                                              16    7355
NP_055166                                                              16    7355
NP_892018                                                              16    7355
XP_005259608                                                           16    7355
XP_005269043                                                           16    7355
XP_006719529                                                           16    7355
XP_005269042                                                           16    7355
XP_005269041                                                           16    7355
NP_861453                                                              15    9605
NP_127493                                                              15    9605
NP_127494                                                              15    9605
NP_001157108                                                           15    9605
NP_001509                                                              15    9605
NP_127492                                                              15    9605
NP_112557                                                              15    9605
NP_003709                                                              15    9605
XP_005250353                                                           15    9605
XP_006716008                                                           15    9605
XP_006716007                                                           15    9605
XP_006716006                                                           15    9605
XP_006716005                                                           15    9605
XP_006716004                                                           15    9605
XP_006716003                                                           15    9605
XP_006716002                                                           15    9605
XP_005250348                                                           15    9605

>NP_005219
          Length = 1210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IKEATSP 9
           ++EATSP
Sbjct: 747 LREATSP 753



>NP_061857
          Length = 1049

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V IKE +SP
Sbjct: 771 VVIKEESSP 779



>NP_055166
          Length = 284

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   IKEATSP 9
           IKE TSP
Sbjct: 99  IKELTSP 105



>NP_892018
          Length = 1059

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           VA+ E TSP
Sbjct: 293 VAVLELTSP 301



>XP_005259608
          Length = 1093

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           VA+ E TSP
Sbjct: 327 VAVLELTSP 335



>XP_005269043
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V IKE +SP
Sbjct: 459 VVIKEESSP 467



>XP_006719529
          Length = 923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V IKE +SP
Sbjct: 645 VVIKEESSP 653



>XP_005269042
          Length = 969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V IKE +SP
Sbjct: 691 VVIKEESSP 699



>XP_005269041
          Length = 1049

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V IKE +SP
Sbjct: 771 VVIKEESSP 779



>NP_861453
          Length = 255

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AIKEATSP 9
           A+KE T+P
Sbjct: 220 AVKEDTTP 227



>NP_127493
          Length = 978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 794 AIKESTS 800



>NP_127494
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 793 AIKESTS 799



>NP_001157108
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 792 AIKESTS 798



>NP_001509
          Length = 957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 773 AIKESTS 779



>NP_127492
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 814 AIKESTS 820



>NP_112557
          Length = 1452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V++KE T P
Sbjct: 578 VSLKEKTKP 586



>NP_003709
          Length = 1512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VAIKEATSP 9
           V++KE T P
Sbjct: 578 VSLKEKTKP 586



>XP_005250353
          Length = 969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 785 AIKESTS 791



>XP_006716008
          Length = 988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 804 AIKESTS 810



>XP_006716007
          Length = 989

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 805 AIKESTS 811



>XP_006716006
          Length = 990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 806 AIKESTS 812



>XP_006716005
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 814 AIKESTS 820



>XP_006716004
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 819 AIKESTS 825



>XP_006716003
          Length = 1009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 825 AIKESTS 831



>XP_006716002
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 826 AIKESTS 832



>XP_005250348
          Length = 1010

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   AIKEATS 8
           AIKE+TS
Sbjct: 826 AIKESTS 832



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.113    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,865
Number of extensions: 730
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)