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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055726                                                              18    1935
NP_004524                                                              16    5631
NP_057256                                                              16    7355
NP_004116                                                              16    7355
NP_002068                                                              15    9605
NP_001240766                                                           15    9605
XP_006717126                                                           15    9605
XP_005251986                                                           15    9605
XP_006717125                                                           15    9605

>NP_055726
          Length = 961

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   VKASEAAA 9
           VKASEAAA
Sbjct: 337 VKASEAAA 344



>NP_004524
          Length = 1141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TVKASEAA 8
           TVKASE A
Sbjct: 449 TVKASEQA 456



>NP_057256
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   TVKASEAAA 9
           T  ASEAAA
Sbjct: 253 TAAASEAAA 261



>NP_004116
          Length = 153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TVKASEAAA 9
           T+K S+AAA
Sbjct: 110 TLKVSQAAA 118



>NP_002068
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TVKASEAA 8
           T+KA EAA
Sbjct: 554 TLKAEEAA 561



>NP_001240766
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  TVKASEAA 8
          +VKASEA+
Sbjct: 19 SVKASEAS 26



>XP_006717126
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TVKASEAA 8
           T+KA EAA
Sbjct: 554 TLKAEEAA 561



>XP_005251986
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TVKASEAA 8
           T+KA EAA
Sbjct: 554 TLKAEEAA 561



>XP_006717125
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TVKASEAA 8
           T+KA EAA
Sbjct: 554 TLKAEEAA 561



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.285   0.0905    0.190 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 180,040
Number of extensions: 116
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)