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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     665
XP_005266703                                                           19     665
NP_005024                                                              17    3301
NP_001029366                                                           17    3301
NP_055906                                                              15    9605
NP_055796                                                              15    9605
XP_006720146                                                           15    9605
XP_005267508                                                           15    9605

>NP_443179
          Length = 2170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    TTSAAQEKR 9
            TTSAAQE+R
Sbjct: 1159 TTSAAQEER 1167



>XP_005266703
          Length = 1501

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TTSAAQEKR 9
           TTSAAQE+R
Sbjct: 490 TTSAAQEER 498



>NP_005024
          Length = 439

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TTSAAQEK 8
           TTSA QEK
Sbjct: 415 TTSAKQEK 422



>NP_001029366
          Length = 456

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   TTSAAQEK 8
           TTSA QEK
Sbjct: 432 TTSAKQEK 439



>NP_055906
          Length = 1720

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TTSAAQEK 8
           TTS+ QEK
Sbjct: 772 TTSSHQEK 779



>NP_055796
          Length = 1946

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    TTSAAQEKR 9
            TTSA+ +KR
Sbjct: 1199 TTSASLKKR 1207



>XP_006720146
          Length = 1226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TTSAAQEK 8
           TTS+ QEK
Sbjct: 772 TTSSHQEK 779



>XP_005267508
          Length = 1773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TTSAAQEK 8
           TTS+ QEK
Sbjct: 772 TTSSHQEK 779



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.286   0.0922    0.203 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 435,196
Number of extensions: 388
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)