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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064714                                                              18    1935
XP_005245479                                                           18    1935
NP_068755                                                              18    1935
NP_060482                                                              17    3301
NP_001166028                                                           17    4312
NP_005886                                                              17    4312
XP_006719803                                                           17    4312
XP_005266222                                                           17    4312
XP_005266221                                                           17    4312
XP_006719802                                                           17    4312
XP_006719801                                                           17    4312
XP_006719800                                                           17    4312
XP_006719799                                                           17    4312
XP_005266219                                                           17    4312
NP_006682                                                              16    7355
NP_003283                                                              16    7355
XP_005245528                                                           16    7355
NP_001166953                                                           15    9605
NP_060521                                                              15    9605
NP_057386                                                              15    9605
NP_001028732                                                           15    9605
NP_036292                                                              15    9605
NP_001265645                                                           15    9605
NP_570722                                                              15    9605
NP_001028731                                                           15    9605
XP_005249607                                                           15    9605
XP_005249606                                                           15    9605
XP_006716104                                                           15    9605
XP_005266987                                                           15    9605
XP_006715521                                                           15    9605
XP_005265259                                                           15    9605

>NP_064714
          Length = 1791

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TTKTAIFST 9
           TT TAIF+T
Sbjct: 171 TTTTAIFTT 179



>XP_005245479
          Length = 1791

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TTKTAIFST 9
           TT TAIF+T
Sbjct: 171 TTTTAIFTT 179



>NP_068755
          Length = 827

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   TTKTAIFST 9
           TT TAIF+T
Sbjct: 171 TTTTAIFTT 179



>NP_060482
          Length = 2108

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    TTKTAIFST 9
            TTKT  FST
Sbjct: 1752 TTKTLSFST 1760



>NP_001166028
          Length = 1134

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 179 TKTRLFST 186



>NP_005886
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 179 TKTRLFST 186



>XP_006719803
          Length = 1407

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TKTAIFST 9
          TKT +FST
Sbjct: 88 TKTRLFST 95



>XP_005266222
          Length = 1458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 139 TKTRLFST 146



>XP_005266221
          Length = 1458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 139 TKTRLFST 146



>XP_006719802
          Length = 1458

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 139 TKTRLFST 146



>XP_006719801
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 179 TKTRLFST 186



>XP_006719800
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 179 TKTRLFST 186



>XP_006719799
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 179 TKTRLFST 186



>XP_005266219
          Length = 1498

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TKTAIFST 9
           TKT +FST
Sbjct: 179 TKTRLFST 186



>NP_006682
          Length = 322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTKTAIFST 9
           TTK  IF+T
Sbjct: 145 TTKDPIFNT 153



>NP_003283
          Length = 2363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TTKTAIFST 9
            +T TA+F T
Sbjct: 1810 STSTAVFGT 1818



>XP_005245528
          Length = 2439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TTKTAIFST 9
            +T TA+F T
Sbjct: 1886 STSTAVFGT 1894



>NP_001166953
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TTKTAIFS 8
           TTK  IFS
Sbjct: 331 TTKNEIFS 338



>NP_060521
          Length = 1066

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTKTAIFST 9
           T +TA FST
Sbjct: 744 TFRTATFST 752



>NP_057386
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TTKTAIFS 8
           TTK  IFS
Sbjct: 332 TTKNEIFS 339



>NP_001028732
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TKTAIF 7
          TKTA+F
Sbjct: 13 TKTAVF 18



>NP_036292
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKTAIFST 9
          T+TA+F T
Sbjct: 92 TQTAVFRT 99



>NP_001265645
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKTAIFST 9
          T+TA+F T
Sbjct: 92 TQTAVFRT 99



>NP_570722
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TKTAIF 7
          TKTA+F
Sbjct: 13 TKTAVF 18



>NP_001028731
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  TKTAIF 7
          TKTA+F
Sbjct: 13 TKTAVF 18



>XP_005249607
          Length = 579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTKTAIFST 9
           T +TA FST
Sbjct: 257 TFRTATFST 265



>XP_005249606
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTKTAIFST 9
           T +TA FST
Sbjct: 556 TFRTATFST 564



>XP_006716104
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TTKTAIFST 9
           T +TA FST
Sbjct: 747 TFRTATFST 755



>XP_005266987
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKTAIFST 9
          T+TA+F T
Sbjct: 92 TQTAVFRT 99



>XP_006715521
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  TKTAIFST 9
          T+TA+F T
Sbjct: 92 TQTAVFRT 99



>XP_005265259
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TTKTAIFS 8
           TTK  IFS
Sbjct: 269 TTKNEIFS 276



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.111    0.274 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 312,430
Number of extensions: 391
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)