Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              19     869
NP_001127854                                                           16    5631
NP_055684                                                              16    5631
NP_001171811                                                           16    7355
NP_001171808                                                           16    7355
NP_003865                                                              16    7355
NP_001171810                                                           16    7355
NP_001360                                                              15    9605
NP_005406                                                              15    9605
NP_057191                                                              15    9605
NP_757385                                                              15    9605
NP_115807                                                              15    9605
NP_001018054                                                           15    9605
NP_079328                                                              15    9605
XP_006714540                                                           15    9605
XP_005248319                                                           15    9605
XP_006712837                                                           15    9605
XP_006712775                                                           15    9605
XP_005263800                                                           15    9605
NP_001014830                                                           15    9605
>NP_000312
          Length = 928

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSVNSTANA 9
           T VNSTANA
Sbjct: 620 TRVNSTANA 628
>NP_001127854
          Length = 1114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SVNSTANA 9
           S+NST+N+
Sbjct: 458 SINSTSNS 465
>NP_055684
          Length = 963

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SVNSTANA 9
           S+NST+N+
Sbjct: 472 SINSTSNS 479
>NP_001171811
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  SVNSTAN 8
          SVNST N
Sbjct: 30 SVNSTCN 36
>NP_001171808
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVNSTAN 8
           SVNST N
Sbjct: 144 SVNSTCN 150
>NP_003865
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVNSTAN 8
           SVNST N
Sbjct: 144 SVNSTCN 150
>NP_001171810
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SVNSTAN 8
           SVNST N
Sbjct: 144 SVNSTCN 150
>NP_001360
          Length = 4624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSVNSTANA 9
           +S+N+ ANA
Sbjct: 927 SSINARANA 935
>NP_005406
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TSVNSTAN 8
           TS++ST N
Sbjct: 342 TSIDSTVN 349
>NP_057191
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TSVNSTAN 8
           +S+NST+N
Sbjct: 475 SSLNSTSN 482
>NP_757385
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSVNST 6
           TSVNST
Sbjct: 538 TSVNST 543
>NP_115807
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TSVNST 6
           TSVNST
Sbjct: 537 TSVNST 542
>NP_001018054
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VNSTAN 8
           +NSTAN
Sbjct: 540 INSTAN 545
>NP_079328
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VNSTAN 8
           +NSTAN
Sbjct: 653 INSTAN 658
>XP_006714540
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   TSVNSTAN 8
           +S+NST+N
Sbjct: 431 SSLNSTSN 438
>XP_005248319
          Length = 4609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSVNSTANA 9
           +S+N+ ANA
Sbjct: 912 SSINARANA 920
>XP_006712837
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   VNSTAN 8
           +NSTAN
Sbjct: 653 INSTAN 658
>XP_006712775
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TSVNSTAN 8
          TS++ST N
Sbjct: 89 TSIDSTVN 96
>XP_005263800
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  TSVNSTAN 8
          TS++ST N
Sbjct: 89 TSIDSTVN 96
>NP_001014830
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  TSVNSTANA 9
          TSV S ANA
Sbjct: 42 TSVLSAANA 50
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.278   0.0863    0.192 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 263,737
Number of extensions: 252
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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