Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     869
NP_001276866                                                           19     869
NP_004439                                                              19     869
NP_001276865                                                           19     869
NP_001005862                                                           19     869
XP_005257197                                                           19     869
XP_006721828                                                           19     869
NP_073568                                                              16    5631
XP_005245510                                                           16    5631
NP_055836                                                              16    7355
NP_055045                                                              16    7355
NP_001247431                                                           16    7355
XP_006720036                                                           16    7355
XP_005268376                                                           16    7355
XP_006720034                                                           16    7355
XP_006720033                                                           16    7355
XP_005268375                                                           16    7355
XP_006720032                                                           16    7355
XP_005248755                                                           16    7355
XP_005248754                                                           16    7355
NP_060375                                                              16    7355
NP_005834                                                              15    9605
NP_056262                                                              15    9605
NP_000867                                                              15    9605
XP_005263454                                                           15    9605
XP_005263453                                                           15    9605
>NP_005219
          Length = 1210

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 751 TSPKANKEI 759
>NP_001276866
          Length = 1055

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 759 TSPKANKEI 767
>NP_004439
          Length = 1255

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 759 TSPKANKEI 767
>NP_001276865
          Length = 1240

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 744 TSPKANKEI 752
>NP_001005862
          Length = 1225

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 729 TSPKANKEI 737
>XP_005257197
          Length = 1225

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 729 TSPKANKEI 737
>XP_006721828
          Length = 1336

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TSSKANKEI 9
           TS KANKEI
Sbjct: 840 TSPKANKEI 848
>NP_073568
          Length = 243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TSSKANKE 8
           T+SKA+KE
Sbjct: 193 TASKASKE 200
>XP_005245510
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TSSKANKE 8
           T+SKA+KE
Sbjct: 192 TASKASKE 199
>NP_055836
          Length = 2723

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    SSKANKE 8
            SSKAN+E
Sbjct: 2303 SSKANQE 2309
>NP_055045
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  SKANKE 8
          SKANKE
Sbjct: 48 SKANKE 53
>NP_001247431
          Length = 589

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  SKANKE 8
          SKANKE
Sbjct: 48 SKANKE 53
>XP_006720036
          Length = 296

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 112 SKGNKEI 118
>XP_005268376
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 493 SKGNKEI 499
>XP_006720034
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 429 SKGNKEI 435
>XP_006720033
          Length = 646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 462 SKGNKEI 468
>XP_005268375
          Length = 655

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 493 SKGNKEI 499
>XP_006720032
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 493 SKGNKEI 499
>XP_005248755
          Length = 2465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    SSKANKE 8
            SSKAN+E
Sbjct: 2045 SSKANQE 2051
>XP_005248754
          Length = 2583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    SSKANKE 8
            SSKAN+E
Sbjct: 2163 SSKANQE 2169
>NP_060375
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SKANKEI 9
           SK NKEI
Sbjct: 493 SKGNKEI 499
>NP_005834
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   TSSKANKE 8
           TSS  NKE
Sbjct: 159 TSSNKNKE 166
>NP_056262
          Length = 1602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SSKANKEI 9
            SSK+NK I
Sbjct: 1471 SSKSNKPI 1478
>NP_000867
          Length = 2491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KANKEI 9
           KANKE+
Sbjct: 167 KANKEV 172
>XP_005263454
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SSKANKEI 9
            SSK+NK I
Sbjct: 1193 SSKSNKPI 1200
>XP_005263453
          Length = 1613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SSKANKEI 9
            SSK+NK I
Sbjct: 1482 SSKSNKPI 1489
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.278   0.0959    0.208 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,062
Number of extensions: 492
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.6 bits)
S2: 28 (15.4 bits)

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