Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_958441                                                              20     390
NP_958439                                                              20     390
NP_005219                                                              20     390
NP_001009609                                                           17    3301
NP_001184033                                                           17    4312
NP_005924                                                              17    4312
XP_006718903                                                           17    4312
XP_006718902                                                           17    4312
NP_001269667                                                           16    5631
NP_001269668                                                           16    5631
NP_001269670                                                           16    5631
NP_038476                                                              16    5631
NP_110385                                                              16    5631
NP_001002010                                                           16    5631
NP_872589                                                              16    5631
XP_006724269                                                           16    5631
XP_006724268                                                           16    5631
XP_006724267                                                           16    5631
XP_006724266                                                           16    5631
XP_005261522                                                           16    5631
NP_149020                                                              16    7355
XP_006724673                                                           16    7355
XP_005271892                                                           16    7355
XP_005271890                                                           16    7355
XP_006718077                                                           16    7355
XP_006718076                                                           16    7355
NP_114412                                                              16    7355
NP_005436                                                              15    9605
NP_067077                                                              15    9605
NP_005692                                                              15    9605
NP_001036053                                                           15    9605
NP_001036046                                                           15    9605
NP_079461                                                              15    9605
NP_079272                                                              15    9605
NP_006505                                                              15    9605
NP_005722                                                              15    9605
NP_690601                                                              15    9605
XP_006721874                                                           15    9605
XP_006721873                                                           15    9605
XP_005257260                                                           15    9605
XP_005257258                                                           15    9605
XP_006721872                                                           15    9605
XP_006721871                                                           15    9605
XP_006721870                                                           15    9605
XP_006721869                                                           15    9605
XP_006718114                                                           15    9605
XP_006718113                                                           15    9605
XP_006718112                                                           15    9605
XP_006718111                                                           15    9605
XP_006718110                                                           15    9605
XP_006713858                                                           15    9605
XP_005265428                                                           15    9605
>NP_958441
          Length = 705

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSGEKTKII 9
           TSG+KTKII
Sbjct: 483 TSGQKTKII 491
>NP_958439
          Length = 628

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSGEKTKII 9
           TSG+KTKII
Sbjct: 483 TSGQKTKII 491
>NP_005219
          Length = 1210

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSGEKTKII 9
           TSG+KTKII
Sbjct: 483 TSGQKTKII 491
>NP_001009609
          Length = 141

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  TSGEKTK 7
          T+GEKTK
Sbjct: 8  TNGEKTK 14
>NP_001184033
          Length = 3972

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GEKTKII 9
            GEKTK++
Sbjct: 2308 GEKTKVL 2314
>NP_005924
          Length = 3969

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GEKTKII 9
            GEKTK++
Sbjct: 2305 GEKTKVL 2311
>XP_006718903
          Length = 2673

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GEKTKII 9
            GEKTK++
Sbjct: 1009 GEKTKVL 1015
>XP_006718902
          Length = 2964

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    GEKTKII 9
            GEKTK++
Sbjct: 1300 GEKTKVL 1306
>NP_001269667
          Length = 750

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 181 SGEKLKVV 188
>NP_001269668
          Length = 915

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 346 SGEKLKVV 353
>NP_001269670
          Length = 659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SGEKTKII 9
          SGEK K++
Sbjct: 88 SGEKLKVV 95
>NP_038476
          Length = 1556

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TSGEKTKII 9
           T GEK KI+
Sbjct: 609 TPGEKMKIL 617
>NP_110385
          Length = 916

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 346 SGEKLKVV 353
>NP_001002010
          Length = 336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  SGEKTKII 9
          +G KTKII
Sbjct: 42 TGRKTKII 49
>NP_872589
          Length = 1524

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TSGEKTKII 9
           T GEK KI+
Sbjct: 577 TPGEKMKIL 585
>XP_006724269
          Length = 639

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SGEKTKII 9
          SGEK K++
Sbjct: 69 SGEKLKVV 76
>XP_006724268
          Length = 742

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 173 SGEKLKVV 180
>XP_006724267
          Length = 743

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 173 SGEKLKVV 180
>XP_006724266
          Length = 828

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 308 SGEKLKVV 315
>XP_005261522
          Length = 874

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           SGEK K++
Sbjct: 346 SGEKLKVV 353
>NP_149020
          Length = 1395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           TSGEK+ I+
Sbjct: 407 TSGEKSLIM 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TSGEKTKI 8
           TSGEK+ I
Sbjct: 513 TSGEKSLI 520
>XP_006724673
          Length = 1396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           TSGEK+ I+
Sbjct: 407 TSGEKSLIM 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   TSGEKTKI 8
           TSGEK+ I
Sbjct: 513 TSGEKSLI 520
>XP_005271892
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SGEKTKI 8
           SGE+TK+
Sbjct: 165 SGERTKL 171
>XP_005271890
          Length = 348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SGEKTKI 8
           SGE+TK+
Sbjct: 254 SGERTKL 260
>XP_006718077
          Length = 352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SGEKTKI 8
           SGE+TK+
Sbjct: 258 SGERTKL 264
>XP_006718076
          Length = 363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SGEKTKI 8
           SGE+TK+
Sbjct: 269 SGERTKL 275
>NP_114412
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SGEKTKI 8
           SGE+TK+
Sbjct: 227 SGERTKL 233
>NP_005436
          Length = 1217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   TSGEKTK 7
           TSGEK++
Sbjct: 248 TSGEKSR 254
>NP_067077
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EKTKII 9
           EKTKII
Sbjct: 212 EKTKII 217
>NP_005692
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GEKTKI 8
           GEKTK+
Sbjct: 219 GEKTKL 224
>NP_001036053
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GEKTKI 8
           GEKTK+
Sbjct: 219 GEKTKL 224
>NP_001036046
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GEKTKI 8
           GEKTK+
Sbjct: 219 GEKTKL 224
>NP_079461
          Length = 1990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 777 GEKTKFL 783
>NP_079272
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           S EKTKI+
Sbjct: 335 SKEKTKIL 342
>NP_006505
          Length = 1956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SGEKTKII 9
            S EKTKI+
Sbjct: 1501 SEEKTKIL 1508
>NP_005722
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  SGEKTKII 9
          +G+KTK++
Sbjct: 49 NGDKTKVM 56
>NP_690601
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  SGEKTKII 9
          +G+KTK++
Sbjct: 49 NGDKTKVM 56
>XP_006721874
          Length = 1399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 851 GEKTKFL 857
>XP_006721873
          Length = 1409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 851 GEKTKFL 857
>XP_005257260
          Length = 1990

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 777 GEKTKFL 783
>XP_005257258
          Length = 2000

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 777 GEKTKFL 783
>XP_006721872
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 814 GEKTKFL 820
>XP_006721871
          Length = 2037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 814 GEKTKFL 820
>XP_006721870
          Length = 2064

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 851 GEKTKFL 857
>XP_006721869
          Length = 2074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GEKTKII 9
           GEKTK +
Sbjct: 851 GEKTKFL 857
>XP_006718114
          Length = 1850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           +SGE+TK +
Sbjct: 225 SSGEQTKAL 233
>XP_006718113
          Length = 1881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           +SGE+TK +
Sbjct: 225 SSGEQTKAL 233
>XP_006718112
          Length = 1889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           +SGE+TK +
Sbjct: 225 SSGEQTKAL 233
>XP_006718111
          Length = 1892

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           +SGE+TK +
Sbjct: 225 SSGEQTKAL 233
>XP_006718110
          Length = 1893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   TSGEKTKII 9
           +SGE+TK +
Sbjct: 225 SSGEQTKAL 233
>XP_006713858
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SGEKTKII 9
           S EKTKI+
Sbjct: 296 SKEKTKIL 303
>XP_005265428
          Length = 1959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    SGEKTKII 9
            S EKTKI+
Sbjct: 1504 SEEKTKIL 1511
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.121    0.291 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 651,589
Number of extensions: 1048
Number of successful extensions: 54
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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