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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              21     229
XP_005262424                                                           21     229
NP_061146                                                              17    4312
NP_954872                                                              17    4312
NP_056380                                                              16    5631
NP_001080                                                              16    7355
NP_071325                                                              16    7355
NP_998778                                                              16    7355
XP_006713920                                                           16    7355
XP_006713919                                                           16    7355
NP_000541                                                              15    9605
NP_596870                                                              15    9605
NP_001164509                                                           15    9605
NP_058633                                                              15    9605
NP_004034                                                              15    9605
NP_055680                                                              15    9605
NP_067043                                                              15    9605
NP_001074003                                                           15    9605
NP_001010969                                                           15    9605
NP_005551                                                              15    9605
XP_006724562                                                           15    9605
XP_005274609                                                           15    9605
XP_006723859                                                           15    9605
XP_005260840                                                           15    9605
XP_005254452                                                           15    9605
XP_005254451                                                           15    9605
XP_005251631                                                           15    9605
XP_005270826                                                           15    9605
NP_001164510                                                           15    9605
NP_631948                                                              15    9605

>NP_722576
          Length = 3080

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSAFYENTT 9
           TSAF+ENTT
Sbjct: 683 TSAFHENTT 691



>XP_005262424
          Length = 2875

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TSAFYENTT 9
           TSAF+ENTT
Sbjct: 478 TSAFHENTT 486



>NP_061146
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AFYENTT 9
           AFY+NT+
Sbjct: 810 AFYDNTS 816



>NP_954872
          Length = 799

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AFYENTT 9
           AFY+NT+
Sbjct: 757 AFYDNTS 763



>NP_056380
          Length = 1812

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SAFYENTT 9
            SAF+++TT
Sbjct: 1331 SAFFDSTT 1338



>NP_001080
          Length = 1704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    SAFYEN 7
            S+FYEN
Sbjct: 1262 SSFYEN 1267



>NP_071325
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAFYENTT 9
           +AFYE+ T
Sbjct: 519 TAFYEDAT 526



>NP_998778
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAFYENTT 9
           +AFYE+ T
Sbjct: 519 TAFYEDAT 526



>XP_006713920
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAFYENTT 9
           +AFYE+ T
Sbjct: 519 TAFYEDAT 526



>XP_006713919
          Length = 701

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAFYENTT 9
           +AFYE+ T
Sbjct: 519 TAFYEDAT 526



>NP_000541
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TSAFYENTT 9
           T  FY N+T
Sbjct: 344 TPPFYSNST 352



>NP_596870
          Length = 5604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5    YENTT 9
            YENTT
Sbjct: 5195 YENTT 5199



>NP_001164509
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3  AFYENT 8
          AFY NT
Sbjct: 82 AFYRNT 87



>NP_058633
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   AFYEN 7
           AFYEN
Sbjct: 795 AFYEN 799



>NP_004034
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3  AFYENT 8
          AFY NT
Sbjct: 82 AFYRNT 87



>NP_055680
          Length = 1401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   YENTT 9
           YENTT
Sbjct: 646 YENTT 650



>NP_067043
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TSAFYENTT 9
           TSA  ENT+
Sbjct: 259 TSAHQENTS 267



>NP_001074003
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    SAFYENTT 9
            +AFY +TT
Sbjct: 1449 NAFYAHTT 1456



>NP_001010969
          Length = 519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAFYENTT 9
           +AF+EN T
Sbjct: 234 NAFHENDT 241



>NP_005551
          Length = 3695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3    AFYENTT 9
            AFY N T
Sbjct: 3205 AFYSNAT 3211



>XP_006724562
          Length = 1326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   AFYEN 7
           AFYEN
Sbjct: 659 AFYEN 663



>XP_005274609
          Length = 1468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   AFYEN 7
           AFYEN
Sbjct: 801 AFYEN 805



>XP_006723859
          Length = 3700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3    AFYENTT 9
            AFY N T
Sbjct: 3205 AFYSNAT 3211



>XP_005260840
          Length = 143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TSAFYENTT 9
           TSA  ENT+
Sbjct: 127 TSAHQENTS 135



>XP_005254452
          Length = 2188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    SAFYENTT 9
            +AFY +TT
Sbjct: 1449 NAFYAHTT 1456



>XP_005254451
          Length = 2214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    SAFYENTT 9
            +AFY +TT
Sbjct: 1449 NAFYAHTT 1456



>XP_005251631
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   TSAFYENTT 9
           T  FY N+T
Sbjct: 344 TPPFYSNST 352



>XP_005270826
          Length = 333

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAFYENTT 9
           +AF+EN T
Sbjct: 234 NAFHENDT 241



>NP_001164510
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3  AFYENT 8
          AFY NT
Sbjct: 82 AFYRNT 87



>NP_631948
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TSAFYE 6
           TSAFY+
Sbjct: 168 TSAFYD 173



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.109    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 496,456
Number of extensions: 690
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)