Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     665
NP_001263689                                                           19     665
NP_001119584                                                           19     665
NP_001119586                                                           19     665
NP_001263625                                                           19     665
NP_001119590                                                           19     665
NP_001263690                                                           19     665
NP_001119585                                                           19     665
NP_001263624                                                           19     665
NP_001119588                                                           19     665
NP_001119587                                                           19     665
NP_001119589                                                           19     665
NP_001005324                                                           17    2528
NP_758519                                                              17    3301
NP_000751                                                              17    3301
NP_724781                                                              17    3301
XP_005270552                                                           17    3301
XP_005270550                                                           17    3301
XP_005270549                                                           17    3301
NP_064581                                                              17    4312
NP_060497                                                              16    5631
XP_005252552                                                           16    5631
XP_006717527                                                           16    5631
XP_006717526                                                           16    5631
XP_005252551                                                           16    5631
XP_006717525                                                           16    5631
XP_006717524                                                           16    5631
XP_005252547                                                           16    5631
XP_005252546                                                           16    5631
XP_006717523                                                           16    5631
XP_006717522                                                           16    5631
XP_006717521                                                           16    5631
XP_006717520                                                           16    5631
NP_060901                                                              16    7355
NP_612455                                                              16    7355
NP_004000                                                              15    9605
NP_003997                                                              15    9605
NP_000100                                                              15    9605
NP_852111                                                              15    9605
NP_000136                                                              15    9605
NP_001012773                                                           15    9605
NP_848611                                                              15    9605
XP_005262186                                                           15    9605
XP_006724538                                                           15    9605
XP_006724537                                                           15    9605
XP_006724536                                                           15    9605
XP_006724535                                                           15    9605
XP_006724534                                                           15    9605
XP_006724533                                                           15    9605
XP_006724532                                                           15    9605
XP_006724531                                                           15    9605
XP_005267190                                                           15    9605
XP_005267189                                                           15    9605
XP_005267187                                                           15    9605
XP_005267185                                                           15    9605
XP_005267184                                                           15    9605
NP_009055                                                              15    9605
>NP_000537
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163
>NP_001263689
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124
>NP_001119584
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163
>NP_001119586
          Length = 341

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163
>NP_001263625
          Length = 302

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124
>NP_001119590
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124
>NP_001263690
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124
>NP_001119585
          Length = 346

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 155 TRVRAMAIY 163
>NP_001263624
          Length = 307

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TRVHAMAIY 9
           TRV AMAIY
Sbjct: 116 TRVRAMAIY 124
>NP_001119588
          Length = 209

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TRVHAMAIY 9
          TRV AMAIY
Sbjct: 23 TRVRAMAIY 31
>NP_001119587
          Length = 261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TRVHAMAIY 9
          TRV AMAIY
Sbjct: 23 TRVRAMAIY 31
>NP_001119589
          Length = 214

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TRVHAMAIY 9
          TRV AMAIY
Sbjct: 23 TRVRAMAIY 31
>NP_001005324
          Length = 309

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TRVHAMAIY 9
           TRVH  A+Y
Sbjct: 193 TRVHKTALY 201
>NP_758519
          Length = 783

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRVHAMA 7
           TR+HAMA
Sbjct: 433 TRLHAMA 439
>NP_000751
          Length = 836

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRVHAMA 7
           TR+HAMA
Sbjct: 433 TRLHAMA 439
>NP_724781
          Length = 863

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRVHAMA 7
           TR+HAMA
Sbjct: 433 TRLHAMA 439
>XP_005270552
          Length = 731

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRVHAMA 7
           TR+HAMA
Sbjct: 301 TRLHAMA 307
>XP_005270550
          Length = 835

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRVHAMA 7
           TR+HAMA
Sbjct: 433 TRLHAMA 439
>XP_005270549
          Length = 841

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TRVHAMA 7
           TR+HAMA
Sbjct: 411 TRLHAMA 417
>NP_064581
          Length = 855

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HAMAIY 9
           HAMA+Y
Sbjct: 611 HAMAVY 616
>NP_060497
          Length = 1039

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 696 TRLHSLALH 704
>XP_005252552
          Length = 701

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 347 TRLHSLALH 355
>XP_006717527
          Length = 721

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 367 TRLHSLALH 375
>XP_006717526
          Length = 753

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 399 TRLHSLALH 407
>XP_005252551
          Length = 902

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 548 TRLHSLALH 556
>XP_006717525
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 708 TRLHSLALH 716
>XP_006717524
          Length = 1072

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 729 TRLHSLALH 737
>XP_005252547
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 724 TRLHSLALH 732
>XP_005252546
          Length = 1083

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 729 TRLHSLALH 737
>XP_006717523
          Length = 1095

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 741 TRLHSLALH 749
>XP_006717522
          Length = 1098

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 744 TRLHSLALH 752
>XP_006717521
          Length = 1099

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 756 TRLHSLALH 764
>XP_006717520
          Length = 1110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   TRVHAMAIY 9
           TR+H++A++
Sbjct: 756 TRLHSLALH 764
>NP_060901
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TRVHAMAIY 9
          T+ HA+ IY
Sbjct: 75 TKKHAIGIY 83
>NP_612455
          Length = 234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VHAMAIY 9
           +HAMA Y
Sbjct: 135 LHAMAFY 141
>NP_004000
          Length = 3681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 65 TRVHAL 70
>NP_003997
          Length = 3685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>NP_000100
          Length = 3677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 61 TRVHAL 66
>NP_852111
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   TRVHAMAIY 9
           TR+H++  Y
Sbjct: 202 TRIHSLPSY 210
>NP_000136
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   TRVHAMAIY 9
           TR+H++  Y
Sbjct: 228 TRIHSLPSY 236
>NP_001012773
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  RVHAMAI 8
          ++HAMA+
Sbjct: 56 QIHAMAV 62
>NP_848611
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   RVHAMAI 8
           RVH MA+
Sbjct: 447 RVHGMAL 453
>XP_005262186
          Length = 307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  RVHAMAI 8
          ++HAMA+
Sbjct: 56 QIHAMAV 62
>XP_006724538
          Length = 3575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_006724537
          Length = 3593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_006724536
          Length = 3657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_006724535
          Length = 3660

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_006724534
          Length = 3668

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_006724533
          Length = 3690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_006724532
          Length = 3695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 61 TRVHAL 66
>XP_006724531
          Length = 3703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 69 TRVHAL 74
>XP_005267190
          Length = 3424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 76 TRVHAL 81
>XP_005267189
          Length = 3425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 90 TRVHAL 95
>XP_005267187
          Length = 3433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 85 TRVHAL 90
>XP_005267185
          Length = 3437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 90 TRVHAL 95
>XP_005267184
          Length = 3438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 90 TRVHAL 95
>NP_009055
          Length = 3433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  TRVHAM 6
          TRVHA+
Sbjct: 85 TRVHAL 90
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.133    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 420,193
Number of extensions: 340
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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