Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003626                                                              17    4312
XP_005270313                                                           17    4312
XP_005270312                                                           17    4312
XP_006718109                                                           17    4312
NP_000101                                                              16    5631
XP_006710460                                                           16    5631
XP_005270619                                                           16    5631
NP_055863                                                              16    7355
NP_116213                                                              16    7355
NP_001135918                                                           16    7355
NP_065208                                                              16    7355
XP_006719359                                                           16    7355
XP_006719358                                                           16    7355
XP_005253915                                                           16    7355
XP_005273533                                                           16    7355
XP_005263884                                                           16    7355
XP_006712870                                                           16    7355
NP_065210                                                              16    7355
NP_065209                                                              16    7355
NP_000028                                                              16    7355
NP_005682                                                              15    9605
NP_064693                                                              15    9605
NP_000187                                                              15    9605
NP_001243838                                                           15    9605
NP_079119                                                              15    9605
NP_001131136                                                           15    9605
NP_057322                                                              15    9605
XP_006719513                                                           15    9605
XP_005253348                                                           15    9605
XP_005253347                                                           15    9605
XP_006719088                                                           15    9605
XP_005253346                                                           15    9605
XP_005253344                                                           15    9605
XP_005253345                                                           15    9605
XP_005253343                                                           15    9605
XP_005253341                                                           15    9605
>NP_003626
          Length = 883

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TPLALRAV 8
           TPLALR++
Sbjct: 739 TPLALRSL 746
>XP_005270313
          Length = 509

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TPLALRAV 8
           TPLALR++
Sbjct: 365 TPLALRSL 372
>XP_005270312
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TPLALRAV 8
           TPLALR++
Sbjct: 739 TPLALRSL 746
>XP_006718109
          Length = 821

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   TPLALRAV 8
           TPLALR++
Sbjct: 677 TPLALRSL 684
>NP_000101
          Length = 1025

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+ALRAV
Sbjct: 772 PIALRAV 778
>XP_006710460
          Length = 889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+ALRAV
Sbjct: 772 PIALRAV 778
>XP_005270619
          Length = 953

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+ALRAV
Sbjct: 700 PIALRAV 706
>NP_055863
          Length = 1923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    PLALRAVL 9
            PLAL AVL
Sbjct: 1440 PLALPAVL 1447
>NP_116213
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  TPLALRAV 8
          TP A+RAV
Sbjct: 36 TPAAVRAV 43
>NP_001135918
          Length = 1897

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TPLALR 6
            TPLALR
Sbjct: 1421 TPLALR 1426
>NP_065208
          Length = 1856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TPLALR 6
            TPLALR
Sbjct: 1380 TPLALR 1385
>XP_006719359
          Length = 1966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    PLALRAVL 9
            PLAL AVL
Sbjct: 1483 PLALPAVL 1490
>XP_006719358
          Length = 1966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    PLALRAVL 9
            PLAL AVL
Sbjct: 1483 PLALPAVL 1490
>XP_005253915
          Length = 1966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    PLALRAVL 9
            PLAL AVL
Sbjct: 1483 PLALPAVL 1490
>XP_005273533
          Length = 1921

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TPLALR 6
            TPLALR
Sbjct: 1421 TPLALR 1426
>XP_005263884
          Length = 554

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  TPLALRAV 8
          TP A+RAV
Sbjct: 36 TPAAVRAV 43
>XP_006712870
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  TPLALRAV 8
          TP A+RAV
Sbjct: 36 TPAAVRAV 43
>NP_065210
          Length = 1719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TPLALR 6
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_065209
          Length = 1881

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TPLALR 6
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_000028
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TPLALR 6
            TPLALR
Sbjct: 1380 TPLALR 1385
>NP_005682
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>NP_064693
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>NP_000187
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  PLALRAVL 9
          P+A RAVL
Sbjct: 79 PVATRAVL 86
>NP_001243838
          Length = 228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  PLALRAVL 9
          PL+LRA L
Sbjct: 90 PLSLRAFL 97
>NP_079119
          Length = 286

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLALRAVL 9
           PL+LRA L
Sbjct: 148 PLSLRAFL 155
>NP_001131136
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLALRAVL 9
           PLAL A+L
Sbjct: 200 PLALDAIL 207
>NP_057322
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLALRAVL 9
           PLAL A+L
Sbjct: 200 PLALDAIL 207
>XP_006719513
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLALRAVL 9
           PLAL A+L
Sbjct: 153 PLALDAIL 160
>XP_005253348
          Length = 1229

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253347
          Length = 1502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_006719088
          Length = 1536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253346
          Length = 1536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253344
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253345
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253343
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
>XP_005253341
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   PLALRAV 8
           P+A+RAV
Sbjct: 252 PIAMRAV 258
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.140    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,692
Number of extensions: 395
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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