Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1135
XP_006716142                                                           18    1135
XP_005250088                                                           18    1135
XP_006716141                                                           18    1135
XP_005250085                                                           18    1135
XP_006716140                                                           18    1135
XP_005250084                                                           18    1135
XP_005250083                                                           18    1135
XP_005250082                                                           18    1135
NP_001035521                                                           17    4312
NP_001073918                                                           16    5631
XP_006718416                                                           16    5631
XP_006718415                                                           16    5631
XP_006718414                                                           16    5631
XP_005253234                                                           16    5631
XP_006718412                                                           16    5631
XP_005253231                                                           16    5631
XP_006717743                                                           16    5631
NP_001129628                                                           16    7355
NP_055725                                                              16    7355
NP_065073                                                              16    7355
XP_006715582                                                           16    7355
NP_001141                                                              15    9605
NP_689725                                                              15    9605
NP_055061                                                              15    9605
NP_689540                                                              15    9605
XP_006724446                                                           15    9605
XP_006724216                                                           15    9605
XP_006724215                                                           15    9605
XP_006724214                                                           15    9605
XP_006724213                                                           15    9605
XP_006724212                                                           15    9605
XP_005254949                                                           15    9605
XP_006720086                                                           15    9605
XP_006720085                                                           15    9605
XP_006720084                                                           15    9605
>NP_733751
          Length = 4911

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2491 TMPSQERFL 2499
>XP_006716142
          Length = 4927

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>XP_005250088
          Length = 4928

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>XP_006716141
          Length = 4959

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>XP_005250085
          Length = 4979

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>XP_006716140
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>XP_005250084
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>XP_005250083
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2507 TMPSQERFL 2515
>XP_005250082
          Length = 4983

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    TMLSQERFL 9
            TM SQERFL
Sbjct: 2508 TMPSQERFL 2516
>NP_001035521
          Length = 106

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  LSQERFL 9
          +SQERFL
Sbjct: 79 ISQERFL 85
>NP_001073918
          Length = 1060

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TMLSQER 7
           T+LSQER
Sbjct: 318 TLLSQER 324
>XP_006718416
          Length = 784

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TMLSQERFL 9
           TMLSQ++ L
Sbjct: 762 TMLSQDQLL 770
>XP_006718415
          Length = 839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TMLSQERFL 9
           TMLSQ++ L
Sbjct: 817 TMLSQDQLL 825
>XP_006718414
          Length = 850

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TMLSQERFL 9
           TMLSQ++ L
Sbjct: 828 TMLSQDQLL 836
>XP_005253234
          Length = 916

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TMLSQERFL 9
           TMLSQ++ L
Sbjct: 894 TMLSQDQLL 902
>XP_006718412
          Length = 927

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TMLSQERFL 9
           TMLSQ++ L
Sbjct: 905 TMLSQDQLL 913
>XP_005253231
          Length = 977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TMLSQERFL 9
           TMLSQ++ L
Sbjct: 955 TMLSQDQLL 963
>XP_006717743
          Length = 1090

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   TMLSQER 7
           T+LSQER
Sbjct: 348 TLLSQER 354
>NP_001129628
          Length = 953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSQERFL 9
           LSQ+RFL
Sbjct: 409 LSQKRFL 415
>NP_055725
          Length = 953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LSQERFL 9
           LSQ+RFL
Sbjct: 409 LSQKRFL 415
>NP_065073
          Length = 2177

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  MLSQERFL 9
          +LS+ERF+
Sbjct: 75 LLSEERFV 82
>XP_006715582
          Length = 2106

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  MLSQERFL 9
          +LS+ERF+
Sbjct: 4  LLSEERFV 11
>NP_001141
          Length = 967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LSQERFL 9
           LSQE FL
Sbjct: 561 LSQEHFL 567
>NP_689725
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TMLSQE 6
           TMLSQE
Sbjct: 219 TMLSQE 224
>NP_055061
          Length = 3014

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    LSQERFL 9
            +SQE+FL
Sbjct: 1214 MSQEKFL 1220
>NP_689540
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMLSQERFL 9
           T  SQ+RFL
Sbjct: 546 THYSQDRFL 554
>XP_006724446
          Length = 3019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    LSQERFL 9
            +SQE+FL
Sbjct: 1214 MSQEKFL 1220
>XP_006724216
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TMLSQE 6
           TMLSQE
Sbjct: 219 TMLSQE 224
>XP_006724215
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  TMLSQE 6
          TMLSQE
Sbjct: 88 TMLSQE 93
>XP_006724214
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TMLSQE 6
           TMLSQE
Sbjct: 106 TMLSQE 111
>XP_006724213
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TMLSQE 6
           TMLSQE
Sbjct: 166 TMLSQE 171
>XP_006724212
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   TMLSQE 6
           TMLSQE
Sbjct: 219 TMLSQE 224
>XP_005254949
          Length = 967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LSQERFL 9
           LSQE FL
Sbjct: 561 LSQEHFL 567
>XP_006720086
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMLSQERFL 9
           T  SQ+RFL
Sbjct: 519 THYSQDRFL 527
>XP_006720085
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMLSQERFL 9
           T  SQ+RFL
Sbjct: 520 THYSQDRFL 528
>XP_006720084
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TMLSQERFL 9
           T  SQ+RFL
Sbjct: 546 THYSQDRFL 554
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.135    0.342 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 394,655
Number of extensions: 369
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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