Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              20     509
NP_056080                                                              17    4312
NP_001165980                                                           17    4312
NP_001165988                                                           17    4312
NP_001026862                                                           17    4312
NP_005815                                                              17    4312
XP_005250165                                                           17    4312
XP_005246453                                                           17    4312
XP_006712435                                                           17    4312
NP_060827                                                              16    5631
NP_001139669                                                           16    5631
NP_001104262                                                           16    7355
NP_004983                                                              16    7355
NP_981953                                                              16    7355
NP_001182209                                                           16    7355
XP_005274738                                                           16    7355
NP_001070665                                                           15    9605
NP_848658                                                              15    9605
NP_060380                                                              15    9605
XP_006716026                                                           15    9605
XP_006716025                                                           15    9605
XP_006716024                                                           15    9605
XP_005264451                                                           15    9605
XP_006712100                                                           15    9605
XP_005264450                                                           15    9605
>NP_005219
          Length = 1210

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TKFKKIKVL 9
           T+FKKIKVL
Sbjct: 710 TEFKKIKVL 718
>NP_056080
          Length = 733

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KFKKIKV 8
           K+KKIKV
Sbjct: 228 KYKKIKV 234
>NP_001165980
          Length = 733

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KFKKIKV 8
           K+KKIKV
Sbjct: 228 KYKKIKV 234
>NP_001165988
          Length = 733

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KFKKIKV 8
           K+KKIKV
Sbjct: 228 KYKKIKV 234
>NP_001026862
          Length = 441

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KFKK+K L
Sbjct: 102 SKFKKLKSL 110
>NP_005815
          Length = 313

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KFKK+K L
Sbjct: 102 SKFKKLKSL 110
>XP_005250165
          Length = 441

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KFKK+K L
Sbjct: 102 SKFKKLKSL 110
>XP_005246453
          Length = 675

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KFKKIKV 8
           K+KKIKV
Sbjct: 170 KYKKIKV 176
>XP_006712435
          Length = 733

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KFKKIKV 8
           K+KKIKV
Sbjct: 228 KYKKIKV 234
>NP_060827
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   TKFKKIKVL 9
           T FKK+K L
Sbjct: 133 TSFKKVKHL 141
>NP_001139669
          Length = 7081

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    TKFKKI 6
            TKFKKI
Sbjct: 1395 TKFKKI 1400
>NP_001104262
          Length = 498

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  KFKKIK 7
          KFKK+K
Sbjct: 39 KFKKVK 44
>NP_004983
          Length = 486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  KFKKIK 7
          KFKK+K
Sbjct: 27 KFKKVK 32
>NP_981953
          Length = 791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KFKKIK 7
           KFKK+K
Sbjct: 643 KFKKVK 648
>NP_001182209
          Length = 287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  TKFKKIKVL 9
          ++FKK+K L
Sbjct: 64 SRFKKLKYL 72
>XP_005274738
          Length = 486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  KFKKIK 7
          KFKK+K
Sbjct: 27 KFKKVK 32
>NP_001070665
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  TKFKKIKV 8
          T++KK+K+
Sbjct: 17 TQWKKVKI 24
>NP_848658
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KF KI+VL
Sbjct: 320 SKFCKIRVL 328
>NP_060380
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKFKKI 6
           TKFKK+
Sbjct: 166 TKFKKL 171
>XP_006716026
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KF KI+VL
Sbjct: 320 SKFCKIRVL 328
>XP_006716025
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KF KI+VL
Sbjct: 320 SKFCKIRVL 328
>XP_006716024
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TKFKKIKVL 9
           +KF KI+VL
Sbjct: 320 SKFCKIRVL 328
>XP_005264451
          Length = 240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKFKKI 6
           TKFKK+
Sbjct: 166 TKFKKL 171
>XP_006712100
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKFKKI 6
           TKFKK+
Sbjct: 166 TKFKKL 171
>XP_005264450
          Length = 468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   TKFKKI 6
           TKFKK+
Sbjct: 166 TKFKKL 171
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.142    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 381,808
Number of extensions: 481
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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