Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001013433                                                           19     869
NP_060785                                                              19     869
NP_361014                                                              19     869
NP_037522                                                              17    2528
NP_001129292                                                           17    2528
XP_006717096                                                           17    2528
XP_005251926                                                           17    2528
NP_065712                                                              17    4312
NP_001035026                                                           16    5631
NP_067017                                                              16    5631
NP_036273                                                              16    5631
NP_001120795                                                           16    5631
NP_872346                                                              16    5631
XP_006724804                                                           16    5631
XP_006724803                                                           16    5631
XP_005262451                                                           16    5631
XP_005266397                                                           16    5631
XP_006716001                                                           16    5631
XP_006711500                                                           16    5631
NP_000836                                                              16    5631
NP_056383                                                              16    5631
NP_061183                                                              16    7355
NP_659418                                                              16    7355
NP_005285                                                              16    7355
NP_005551                                                              16    7355
XP_006723861                                                           16    7355
XP_006723860                                                           16    7355
XP_006723859                                                           16    7355
XP_005252872                                                           16    7355
XP_005252871                                                           16    7355
XP_006718231                                                           16    7355
XP_005252869                                                           16    7355
XP_005252868                                                           16    7355
XP_006718230                                                           16    7355
XP_005251990                                                           16    7355
NP_004854                                                              15    9605
NP_006080                                                              15    9605
NP_078997                                                              15    9605
NP_757385                                                              15    9605
NP_115807                                                              15    9605
XP_006724521                                                           15    9605
XP_006724520                                                           15    9605
XP_006718776                                                           15    9605
XP_006718775                                                           15    9605
NP_060797                                                              15    9605
>NP_001013433
          Length = 589

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  TFGYLRAAN 9
          TFG LRAAN
Sbjct: 90 TFGDLRAAN 98
>NP_060785
          Length = 627

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TFGYLRAAN 9
           TFG LRAAN
Sbjct: 128 TFGDLRAAN 136
>NP_361014
          Length = 707

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   TFGYLRAAN 9
           TFG LRAAN
Sbjct: 208 TFGDLRAAN 216
>NP_037522
          Length = 1383

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TFGYLRAAN 9
            TFGYL+  N
Sbjct: 1084 TFGYLQRQN 1092
>NP_001129292
          Length = 1320

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TFGYLRAAN 9
            TFGYL+  N
Sbjct: 1021 TFGYLQRQN 1029
>XP_006717096
          Length = 1217

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TFGYLRAAN 9
            TFGYL+  N
Sbjct: 1084 TFGYLQRQN 1092
>XP_005251926
          Length = 1383

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    TFGYLRAAN 9
            TFGYL+  N
Sbjct: 1084 TFGYLQRQN 1092
>NP_065712
          Length = 198

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  FGYLRAAN 9
          FGYLR  N
Sbjct: 46 FGYLRNKN 53
>NP_001035026
          Length = 874

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 352 FGYLKAS 358
>NP_067017
          Length = 507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  TFGYLRA 7
          TFG++RA
Sbjct: 71 TFGWIRA 77
>NP_036273
          Length = 887

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 365 FGYLKAS 371
>NP_001120795
          Length = 908

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GYLRAAN 9
           GY+RA N
Sbjct: 444 GYIRAVN 450
>NP_872346
          Length = 861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 369 FGYLKAS 375
>XP_006724804
          Length = 854

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 369 FGYLKAS 375
>XP_006724803
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 369 FGYLKAS 375
>XP_005262451
          Length = 898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 369 FGYLKAS 375
>XP_005266397
          Length = 709

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FGYL+A+
Sbjct: 187 FGYLKAS 193
>XP_006716001
          Length = 908

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GYLRAAN 9
           GY+RA N
Sbjct: 444 GYIRAVN 450
>XP_006711500
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  TFGYLRA 7
          T+G+LRA
Sbjct: 20 TYGFLRA 26
>NP_000836
          Length = 908

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GYLRAAN 9
           GY+RA N
Sbjct: 444 GYIRAVN 450
>NP_056383
          Length = 567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GYLRAA 8
           GYLRAA
Sbjct: 240 GYLRAA 245
>NP_061183
          Length = 485

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  TFGYLRA 7
          T+GY RA
Sbjct: 71 TYGYARA 77
>NP_659418
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 151 FGFLRAS 157
>NP_005285
          Length = 349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2  FGYLRAAN 9
          FGYL   N
Sbjct: 19 FGYLETVN 26
>NP_005551
          Length = 3695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TFGYLR 6
            +FGYLR
Sbjct: 1385 SFGYLR 1390
>XP_006723861
          Length = 2300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TFGYLR 6
            +FGYLR
Sbjct: 1385 SFGYLR 1390
>XP_006723860
          Length = 2586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TFGYLR 6
            +FGYLR
Sbjct: 1385 SFGYLR 1390
>XP_006723859
          Length = 3700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    TFGYLR 6
            +FGYLR
Sbjct: 1385 SFGYLR 1390
>XP_005252872
          Length = 130

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 115 FGFLRAS 121
>XP_005252871
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 151 FGFLRAS 157
>XP_006718231
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 151 FGFLRAS 157
>XP_005252869
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 151 FGFLRAS 157
>XP_005252868
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 151 FGFLRAS 157
>XP_006718230
          Length = 202

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FGYLRAA 8
           FG+LRA+
Sbjct: 187 FGFLRAS 193
>XP_005251990
          Length = 349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2  FGYLRAAN 9
          FGYL   N
Sbjct: 19 FGYLETVN 26
>NP_004854
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  FGYLR 6
          FGYLR
Sbjct: 84 FGYLR 88
>NP_006080
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FGYLRAAN 9
           FGYL+  N
Sbjct: 410 FGYLKPDN 417
>NP_078997
          Length = 3616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   TFGYLRA 7
           +FGY+R+
Sbjct: 286 SFGYIRS 292
>NP_757385
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FGYLRAA 8
          FG LRAA
Sbjct: 66 FGQLRAA 72
>NP_115807
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FGYLRAA 8
          FG LRAA
Sbjct: 66 FGQLRAA 72
>XP_006724521
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FGYLRAAN 9
           FGYL+  N
Sbjct: 410 FGYLKPDN 417
>XP_006724520
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FGYLRAAN 9
           FGYL+  N
Sbjct: 410 FGYLKPDN 417
>XP_006718776
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FGYLRAA 8
          FG LRAA
Sbjct: 66 FGQLRAA 72
>XP_006718775
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FGYLRAA 8
          FG LRAA
Sbjct: 66 FGQLRAA 72
>NP_060797
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  FGYLR 6
          FGYLR
Sbjct: 76 FGYLR 80
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.139    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 481,044
Number of extensions: 548
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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