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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              20     299
XP_005268094                                                           20     299
NP_000224                                                              17    2528
XP_005264366                                                           17    2528
XP_006712078                                                           17    2528
NP_001008781                                                           16    7355
XP_006718827                                                           16    7355
NP_683704                                                              15    9605
NP_078862                                                              15    9605
NP_683706                                                              15    9605
NP_683707                                                              15    9605
XP_006710382                                                           15    9605
XP_006710381                                                           15    9605

>NP_000360
          Length = 764

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TFAMRLDQK 9
           TFAMRLD+K
Sbjct: 524 TFAMRLDRK 532



>XP_005268094
          Length = 764

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   TFAMRLDQK 9
           TFAMRLD+K
Sbjct: 524 TFAMRLDRK 532



>NP_000224
          Length = 699

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TFAMRLDQK 9
           T+A+ LDQK
Sbjct: 469 TYAIHLDQK 477



>XP_005264366
          Length = 380

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TFAMRLDQK 9
           T+A+ LDQK
Sbjct: 150 TYAIHLDQK 158



>XP_006712078
          Length = 389

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   TFAMRLDQK 9
           T+A+ LDQK
Sbjct: 159 TYAIHLDQK 167



>NP_001008781
          Length = 4557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TFAMRLDQK 9
            TFA++ +QK
Sbjct: 3585 TFALKSEQK 3593



>XP_006718827
          Length = 3479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    TFAMRLDQK 9
            TFA++ +QK
Sbjct: 2475 TFALKSEQK 2483



>NP_683704
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TFAMRLDQK 9
          TF + +DQK
Sbjct: 50 TFTITVDQK 58



>NP_078862
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TFAMRLDQK 9
          TF + +DQK
Sbjct: 67 TFTITVDQK 75



>NP_683706
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TFAMRLDQK 9
          TF + +DQK
Sbjct: 67 TFTITVDQK 75



>NP_683707
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TFAMRLDQK 9
          TF + +DQK
Sbjct: 67 TFTITVDQK 75



>XP_006710382
          Length = 713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TFAMRLDQK 9
          TF + +DQK
Sbjct: 67 TFTITVDQK 75



>XP_006710381
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  TFAMRLDQK 9
          TF + +DQK
Sbjct: 67 TFTITVDQK 75



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.135    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 387,842
Number of extensions: 488
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)