Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_078914                                                              17    2528
NP_001243764                                                           17    2528
NP_149078                                                              17    3301
NP_055178                                                              17    4312
NP_001264984                                                           17    4312
XP_005266395                                                           17    4312
NP_001094857                                                           17    4312
NP_055904                                                              16    5631
NP_004757                                                              16    5631
XP_006715095                                                           16    5631
XP_006715094                                                           16    5631
XP_006715093                                                           16    5631
XP_006715092                                                           16    5631
NP_001013754                                                           16    7355
NP_872332                                                              16    7355
NP_056541                                                              16    7355
XP_006720125                                                           16    7355
XP_005267432                                                           16    7355
XP_005267431                                                           16    7355
XP_005267430                                                           16    7355
XP_006719830                                                           16    7355
NP_694958                                                              16    7355
NP_001128130                                                           16    7355
NP_001053                                                              16    7355
NP_002979                                                              15    9605
NP_055768                                                              15    9605
NP_001121698                                                           15    9605
NP_001164120                                                           15    9605
NP_001164121                                                           15    9605
NP_112483                                                              15    9605
NP_064547                                                              15    9605
NP_001186628                                                           15    9605
NP_060089                                                              15    9605
NP_061903                                                              15    9605
XP_005262364                                                           15    9605
XP_005262358                                                           15    9605
XP_005268376                                                           15    9605
XP_005266351                                                           15    9605
XP_006719841                                                           15    9605
XP_006714716                                                           15    9605
XP_006712519                                                           15    9605
XP_006712518                                                           15    9605
NP_002539                                                              15    9605
NP_001028747                                                           15    9605
NP_001004479                                                           15    9605
>NP_078914
          Length = 415

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  CLIYTKS 8
          CL+YTK+
Sbjct: 33 CLLYTKT 39
>NP_001243764
          Length = 427

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  CLIYTKS 8
          CL+YTK+
Sbjct: 45 CLLYTKT 51
>NP_149078
          Length = 416

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   TCLIYTKS 8
           TC++YT S
Sbjct: 316 TCVLYTPS 323
>NP_055178
          Length = 4579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TCLIYTKSL 9
            TC + TKS+
Sbjct: 3428 TCYVLTKSI 3436
>NP_001264984
          Length = 4432

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TCLIYTKSL 9
            TC + TKS+
Sbjct: 3281 TCYVLTKSI 3289
>XP_005266395
          Length = 4588

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    TCLIYTKSL 9
            TC + TKS+
Sbjct: 3437 TCYVLTKSI 3445
>NP_001094857
          Length = 137

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  TCLIYTKSL 9
          TCL YT  L
Sbjct: 73 TCLAYTLKL 81
>NP_055904
          Length = 2517

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    TCLIYT 6
            TCL YT
Sbjct: 1314 TCLFYT 1319
>NP_004757
          Length = 906

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CLIYTKSL 9
           C IYT S+
Sbjct: 533 CFIYTSSV 540
>XP_006715095
          Length = 2046

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   TCLIYT 6
           TCL YT
Sbjct: 843 TCLFYT 848
>XP_006715094
          Length = 2407

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    TCLIYT 6
            TCL YT
Sbjct: 1204 TCLFYT 1209
>XP_006715093
          Length = 2459

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    TCLIYT 6
            TCL YT
Sbjct: 1256 TCLFYT 1261
>XP_006715092
          Length = 2490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    TCLIYT 6
            TCL YT
Sbjct: 1287 TCLFYT 1292
>NP_001013754
          Length = 846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   CLIYT 6
           CLIYT
Sbjct: 717 CLIYT 721
>NP_872332
          Length = 167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CLIYT 6
          CLIYT
Sbjct: 77 CLIYT 81
>NP_056541
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   TCLIYT 6
           TCL+Y+
Sbjct: 391 TCLVYS 396
>XP_006720125
          Length = 167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CLIYT 6
          CLIYT
Sbjct: 77 CLIYT 81
>XP_005267432
          Length = 167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CLIYT 6
          CLIYT
Sbjct: 77 CLIYT 81
>XP_005267431
          Length = 167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CLIYT 6
          CLIYT
Sbjct: 77 CLIYT 81
>XP_005267430
          Length = 167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CLIYT 6
          CLIYT
Sbjct: 77 CLIYT 81
>XP_006719830
          Length = 428

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   TCLIYTK 7
           +CLIY K
Sbjct: 401 SCLIYLK 407
>NP_694958
          Length = 364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           CLIY K+
Sbjct: 114 CLIYRKT 120
>NP_001128130
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           CLIY K+
Sbjct: 115 CLIYRKT 121
>NP_001053
          Length = 433

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   IYTKSL 9
           IYTKSL
Sbjct: 219 IYTKSL 224
>NP_002979
          Length = 89

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2  CLIYT 6
          CL+YT
Sbjct: 31 CLVYT 35
>NP_055768
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   TCLIYTKSL 9
           TC  Y KSL
Sbjct: 193 TCEEYVKSL 201
>NP_001121698
          Length = 928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   TCLIYTKSL 9
           TC  Y KSL
Sbjct: 163 TCEEYVKSL 171
>NP_001164120
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           C IYT+S
Sbjct: 487 CSIYTQS 493
>NP_001164121
          Length = 675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           C IYT+S
Sbjct: 445 CSIYTQS 451
>NP_112483
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           C IYT+S
Sbjct: 504 CSIYTQS 510
>NP_064547
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   CLIYTKSL 9
           C++YT+ L
Sbjct: 665 CVVYTELL 672
>NP_001186628
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   CLIYTKSL 9
           C++YT+ L
Sbjct: 492 CVVYTELL 499
>NP_060089
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CLIYTKSL 9
           C IY K+L
Sbjct: 420 CRIYVKNL 427
>NP_061903
          Length = 1369

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    LIYTKSL 9
            +IYTKS+
Sbjct: 1231 IIYTKSV 1237
>XP_005262364
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           C IYT+S
Sbjct: 202 CSIYTQS 208
>XP_005262358
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           C IYT+S
Sbjct: 504 CSIYTQS 510
>XP_005268376
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CLIYTKSL 9
           CLI+T S+
Sbjct: 526 CLIWTTSV 533
>XP_005266351
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  TCLIYTKSL 9
          TC  Y KSL
Sbjct: 31 TCEEYVKSL 39
>XP_006719841
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  TCLIYTKSL 9
          TC  Y KSL
Sbjct: 70 TCEEYVKSL 78
>XP_006714716
          Length = 1318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    LIYTKSL 9
            +IYTKS+
Sbjct: 1180 IIYTKSV 1186
>XP_006712519
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           CL+Y K+
Sbjct: 99  CLVYCKN 105
>XP_006712518
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           CL+Y K+
Sbjct: 99  CLVYCKN 105
>NP_002539
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   CLIYTKSL 9
           C++Y K L
Sbjct: 256 CMVYLKPL 263
>NP_001028747
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           CL+Y K+
Sbjct: 99  CLVYCKN 105
>NP_001004479
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   CLIYTKS 8
           C+ YT+S
Sbjct: 207 CIFYTQS 213
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.136    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 571,243
Number of extensions: 983
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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