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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001020029                                                           17    3301
NP_055256                                                              17    3301
NP_055200                                                              17    3301
NP_000338                                                              17    3301
NP_001185487                                                           17    3301
XP_006723209                                                           17    3301
XP_006723203                                                           17    3301
XP_006720304                                                           17    3301
XP_005268080                                                           17    3301
NP_149078                                                              16    5631
NP_005268                                                              16    7355
NP_963885                                                              16    7355
NP_001248377                                                           16    7355
NP_001248376                                                           16    7355
NP_963886                                                              16    7355
XP_006714246                                                           16    7355
NP_001136272                                                           15    9605
NP_660341                                                              15    9605
NP_001138498                                                           15    9605
NP_001185744                                                           15    9605
NP_055323                                                              15    9605
NP_976031                                                              15    9605
XP_005275558                                                           15    9605
XP_005275391                                                           15    9605
XP_005275259                                                           15    9605
XP_005274965                                                           15    9605
XP_005272815                                                           15    9605
XP_005274885                                                           15    9605
XP_005261394                                                           15    9605
XP_005261392                                                           15    9605
XP_005248836                                                           15    9605
NP_689639                                                              15    9605

>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VNVTAFTS 9
            VNVTAFTS
Sbjct: 1518 VNVTAFTS 1525



>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    VNVTAFTS 9
            VNVTAFTS
Sbjct: 1313 VNVTAFTS 1320



>NP_001020029
          Length = 2328

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           VNVT FTS
Sbjct: 193 VNVTNFTS 200



>NP_055256
          Length = 463

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SVNVTAFT 8
           SVNVTA T
Sbjct: 136 SVNVTALT 143



>NP_055200
          Length = 467

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SVNVTAFT 8
           SVNVTA T
Sbjct: 140 SVNVTALT 147



>NP_000338
          Length = 2137

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           VNVT FTS
Sbjct: 193 VNVTNFTS 200



>NP_001185487
          Length = 479

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SVNVTAFT 8
           SVNVTA T
Sbjct: 136 SVNVTALT 143



>XP_006723209
          Length = 481

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SVNVTAFT 8
           SVNVTA T
Sbjct: 136 SVNVTALT 143



>XP_006723203
          Length = 343

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SVNVTAFT 8
           SVNVTA T
Sbjct: 140 SVNVTALT 147



>XP_006720304
          Length = 2328

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           VNVT FTS
Sbjct: 193 VNVTNFTS 200



>XP_005268080
          Length = 2328

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           VNVT FTS
Sbjct: 193 VNVTNFTS 200



>NP_149078
          Length = 416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SVNVTAFTS 9
           S+NVT+F S
Sbjct: 202 SLNVTSFDS 210



>NP_005268
          Length = 278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           + VTAFTS
Sbjct: 142 LGVTAFTS 149



>NP_963885
          Length = 278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           + VTAFTS
Sbjct: 142 LGVTAFTS 149



>NP_001248377
          Length = 271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           + VTAFTS
Sbjct: 135 LGVTAFTS 142



>NP_001248376
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           + VTAFTS
Sbjct: 107 LGVTAFTS 114



>NP_963886
          Length = 267

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNVTAFTS 9
           + VTAFTS
Sbjct: 131 LGVTAFTS 138



>XP_006714246
          Length = 215

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VNVTAFTS 9
          + VTAFTS
Sbjct: 79 LGVTAFTS 86



>NP_001136272
          Length = 3144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    SVNVTAFT 8
            S+N  AFT
Sbjct: 2230 SINTNAFT 2237



>NP_660341
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNVTAFTS 9
           +VN+T FT+
Sbjct: 296 NVNLTIFTA 304



>NP_001138498
          Length = 2299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SVNVTAFT 8
            SV +TAFT
Sbjct: 1609 SVVITAFT 1616



>NP_001185744
          Length = 1773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 25 SENTTAFT 32



>NP_055323
          Length = 190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNVTAFTS 9
           +VN+T FT+
Sbjct: 113 NVNLTIFTA 121



>NP_976031
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VNVTAF 7
           VN+TAF
Sbjct: 331 VNITAF 336



>XP_005275558
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>XP_005275391
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>XP_005275259
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>XP_005274965
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>XP_005272815
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>XP_005274885
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>XP_005261394
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNVTAFTS 9
           +VN+T FT+
Sbjct: 125 NVNLTIFTA 133



>XP_005261392
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNVTAFTS 9
           +VN+T FT+
Sbjct: 113 NVNLTIFTA 121



>XP_005248836
          Length = 1776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SVNVTAFT 8
          S N TAFT
Sbjct: 28 SENTTAFT 35



>NP_689639
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SVNVTAFTS 9
           +VN+T FT+
Sbjct: 309 NVNLTIFTA 317



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.109    0.266 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,300
Number of extensions: 553
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)