Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1135
NP_001164560                                                           17    2528
NP_001186732                                                           17    4312
NP_077298                                                              17    4312
NP_001338                                                              17    4312
NP_085146                                                              17    4312
NP_663693                                                              17    4312
XP_006712165                                                           17    4312
NP_056471                                                              17    4312
NP_006028                                                              16    5631
NP_001019554                                                           16    5631
NP_055718                                                              16    5631
XP_006719740                                                           16    5631
XP_006719739                                                           16    5631
XP_006719738                                                           16    5631
XP_005269272                                                           16    5631
XP_006712943                                                           16    5631
XP_006712942                                                           16    5631
XP_006712941                                                           16    5631
XP_006712940                                                           16    5631
NP_001278253                                                           16    7355
NP_001158238                                                           16    7355
NP_008816                                                              16    7355
NP_003120                                                              16    7355
XP_006722053                                                           16    7355
XP_006722052                                                           16    7355
XP_005256014                                                           16    7355
XP_005251866                                                           16    7355
XP_005251865                                                           16    7355
XP_005251864                                                           16    7355
NP_055745                                                              16    7355
NP_008941                                                              15    9605
NP_001278405                                                           15    9605
NP_001984                                                              15    9605
NP_620416                                                              15    9605
NP_001188394                                                           15    9605
NP_001137468                                                           15    9605
NP_004530                                                              15    9605
NP_001107580                                                           15    9605
NP_775798                                                              15    9605
NP_003282                                                              15    9605
NP_001243537                                                           15    9605
XP_006725362                                                           15    9605
XP_006722616                                                           15    9605
XP_005266833                                                           15    9605
XP_005266831                                                           15    9605
XP_005266830                                                           15    9605
XP_005266757                                                           15    9605
XP_006721713                                                           15    9605
XP_005267440                                                           15    9605
XP_005267439                                                           15    9605
XP_005267438                                                           15    9605
XP_005254130                                                           15    9605
XP_005254129                                                           15    9605
XP_005254128                                                           15    9605
XP_005254127                                                           15    9605
XP_005247772                                                           15    9605
>NP_004295
          Length = 1620

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SVHKLRRAK 9
           SV KLRRAK
Sbjct: 131 SVRKLRRAK 139
>NP_001164560
          Length = 869

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SVHKLRRA 8
           S+HKLR+A
Sbjct: 802 SLHKLRKA 809
>NP_001186732
          Length = 294

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  VHKLRRAK 9
          +HKLRR +
Sbjct: 46 IHKLRRLR 53
>NP_077298
          Length = 300

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  VHKLRRAK 9
          +HKLRR +
Sbjct: 52 IHKLRRLR 59
>NP_001338
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VHKLRRAK 9
           VH LR+AK
Sbjct: 910 VHMLRKAK 917
>NP_085146
          Length = 348

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VHKLRRAK 9
           VH LRR+K
Sbjct: 244 VHTLRRSK 251
>NP_663693
          Length = 351

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VHKLRRAK 9
           VH LRR+K
Sbjct: 247 VHTLRRSK 254
>XP_006712165
          Length = 300

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  VHKLRRAK 9
          +HKLRR +
Sbjct: 52 IHKLRRLR 59
>NP_056471
          Length = 1882

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VHKLRRAK 9
           +H+LRR K
Sbjct: 722 IHRLRRKK 729
>NP_006028
          Length = 1084

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           S+HKLR+
Sbjct: 452 SIHKLRQ 458
>NP_001019554
          Length = 2385

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VHKLRR 7
            +HKLRR
Sbjct: 1571 IHKLRR 1576
>NP_055718
          Length = 2363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VHKLRR 7
            +HKLRR
Sbjct: 1571 IHKLRR 1576
>XP_006719740
          Length = 1863

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VHKLRR 7
            +HKLRR
Sbjct: 1071 IHKLRR 1076
>XP_006719739
          Length = 2306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VHKLRR 7
            +HKLRR
Sbjct: 1514 IHKLRR 1519
>XP_006719738
          Length = 2340

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VHKLRR 7
            +HKLRR
Sbjct: 1548 IHKLRR 1553
>XP_005269272
          Length = 2356

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    VHKLRR 7
            +HKLRR
Sbjct: 1571 IHKLRR 1576
>XP_006712943
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           S+HKLR+
Sbjct: 430 SIHKLRQ 436
>XP_006712942
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           S+HKLR+
Sbjct: 430 SIHKLRQ 436
>XP_006712941
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           S+HKLR+
Sbjct: 457 SIHKLRQ 463
>XP_006712940
          Length = 1108

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           S+HKLR+
Sbjct: 476 SIHKLRQ 482
>NP_001278253
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    HKLRRAK 9
            HKL RAK
Sbjct: 2212 HKLLRAK 2218
>NP_001158238
          Length = 2789

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VHKLRRA 8
           +HKL+RA
Sbjct: 656 LHKLKRA 662
>NP_008816
          Length = 3703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VHKLRRA 8
            +HKL+RA
Sbjct: 1570 LHKLKRA 1576
>NP_003120
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SVHKLRRA 8
           S+H+LR+A
Sbjct: 483 SLHQLRKA 490
>XP_006722053
          Length = 2608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    HKLRRAK 9
            HKL RAK
Sbjct: 2181 HKLLRAK 2187
>XP_006722052
          Length = 2631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    HKLRRAK 9
            HKL RAK
Sbjct: 2204 HKLLRAK 2210
>XP_005256014
          Length = 3702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VHKLRRA 8
            +HKL+RA
Sbjct: 1569 LHKLKRA 1575
>XP_005251866
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           SVH++RR
Sbjct: 327 SVHRVRR 333
>XP_005251865
          Length = 682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           SVH++RR
Sbjct: 388 SVHRVRR 394
>XP_005251864
          Length = 683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           SVH++RR
Sbjct: 389 SVHRVRR 395
>NP_055745
          Length = 683

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SVHKLRR 7
           SVH++RR
Sbjct: 389 SVHRVRR 395
>NP_008941
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HK+RRA
Sbjct: 345 HKVRRA 350
>NP_001278405
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HK+RRA
Sbjct: 237 HKVRRA 242
>NP_001984
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVHKLRRA 8
           S+HK R+A
Sbjct: 273 SLHKCRKA 280
>NP_620416
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HKLRR+
Sbjct: 418 HKLRRS 423
>NP_001188394
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HKLRR+
Sbjct: 418 HKLRRS 423
>NP_001137468
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  SVHKLR 6
          SVHK+R
Sbjct: 65 SVHKIR 70
>NP_004530
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VHKLRR 7
           VH+LRR
Sbjct: 134 VHRLRR 139
>NP_001107580
          Length = 569

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VHKLRRAK 9
           +H L+RAK
Sbjct: 347 LHDLKRAK 354
>NP_775798
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  HKLRRAK 9
          H+LRRA+
Sbjct: 75 HQLRRAQ 81
>NP_003282
          Length = 1249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVHKLRRA 8
           +VH +RRA
Sbjct: 474 TVHSVRRA 481
>NP_001243537
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VHKLRRA 8
           VHKLR A
Sbjct: 318 VHKLRPA 324
>XP_006725362
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HK+RRA
Sbjct: 159 HKVRRA 164
>XP_006722616
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HKLRR+
Sbjct: 243 HKLRRS 248
>XP_005266833
          Length = 524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HKLRR+
Sbjct: 418 HKLRRS 423
>XP_005266831
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HKLRR+
Sbjct: 418 HKLRRS 423
>XP_005266830
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HKLRR+
Sbjct: 418 HKLRRS 423
>XP_005266757
          Length = 547

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VHKLRR 7
           VH+LRR
Sbjct: 133 VHRLRR 138
>XP_006721713
          Length = 413

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HKLRRA 8
           HK+RRA
Sbjct: 159 HKVRRA 164
>XP_005267440
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  HKLRRAK 9
          H+LRRA+
Sbjct: 75 HQLRRAQ 81
>XP_005267439
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HKLRRAK 9
           H+LRRA+
Sbjct: 107 HQLRRAQ 113
>XP_005267438
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   HKLRRAK 9
           H+LRRA+
Sbjct: 107 HQLRRAQ 113
>XP_005254130
          Length = 1162

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVHKLRRA 8
           +VH +RRA
Sbjct: 474 TVHSVRRA 481
>XP_005254129
          Length = 1262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVHKLRRA 8
           +VH +RRA
Sbjct: 474 TVHSVRRA 481
>XP_005254128
          Length = 1291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVHKLRRA 8
           +VH +RRA
Sbjct: 474 TVHSVRRA 481
>XP_005254127
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SVHKLRRA 8
           +VH +RRA
Sbjct: 474 TVHSVRRA 481
>XP_005247772
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SVHKLRRAK 9
           +VH+LR+++
Sbjct: 660 TVHELRKSR 668
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.127    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 616,584
Number of extensions: 857
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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