Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              18    1135
XP_006715292                                                           18    1135
XP_005258982                                                           17    4312
NP_001099040                                                           17    4312
NP_004720                                                              16    5631
NP_001164607                                                           16    5631
NP_001164606                                                           16    5631
NP_055685                                                              16    5631
NP_001077090                                                           16    5631
NP_001268425                                                           16    7355
NP_733765                                                              16    7355
NP_001672                                                              16    7355
NP_001273004                                                           16    7355
NP_001268644                                                           16    7355
NP_002625                                                              16    7355
NP_004311                                                              16    7355
NP_775293                                                              16    7355
NP_777613                                                              16    7355
NP_777617                                                              16    7355
NP_777614                                                              16    7355
NP_005164                                                              16    7355
NP_777618                                                              16    7355
NP_777615                                                              16    7355
XP_005256713                                                           16    7355
XP_005253945                                                           16    7355
XP_006713871                                                           16    7355
XP_006713870                                                           16    7355
NP_203528                                                              16    7355
NP_777616                                                              16    7355
NP_001531                                                              15    9605
NP_001107228                                                           15    9605
NP_001107227                                                           15    9605
NP_001944                                                              15    9605
NP_001244917                                                           15    9605
NP_005216                                                              15    9605
NP_060390                                                              15    9605
NP_001071131                                                           15    9605
NP_001277005                                                           15    9605
NP_001277004                                                           15    9605
NP_001071132                                                           15    9605
NP_061870                                                              15    9605
NP_114107                                                              15    9605
NP_001116108                                                           15    9605
NP_068580                                                              15    9605
NP_060733                                                              15    9605
NP_065880                                                              15    9605
NP_001129392                                                           15    9605
NP_653213                                                              15    9605
NP_001193486                                                           15    9605
XP_006722447                                                           15    9605
XP_005251580                                                           15    9605
XP_006712103                                                           15    9605
XP_006712102                                                           15    9605
NP_115976                                                              15    9605
>NP_115927
          Length = 1321

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           STADG VEL
Sbjct: 463 STADGRVEL 471
>XP_006715292
          Length = 759

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           STADG VEL
Sbjct: 458 STADGRVEL 466
>XP_005258982
          Length = 201

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TADGLVEL 9
           TADG+V L
Sbjct: 124 TADGMVHL 131
>NP_001099040
          Length = 375

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TADGLVEL 9
           TADG+V L
Sbjct: 298 TADGMVHL 305
>NP_004720
          Length = 694

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T +GLVEL
Sbjct: 392 ATIEGLVEL 400
>NP_001164607
          Length = 694

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T +GLVEL
Sbjct: 392 ATIEGLVEL 400
>NP_001164606
          Length = 694

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T +GLVEL
Sbjct: 392 ATIEGLVEL 400
>NP_055685
          Length = 1239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TADGLVEL 9
           TA+GLV+L
Sbjct: 418 TAEGLVKL 425
>NP_001077090
          Length = 1239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TADGLVEL 9
           TA+GLV+L
Sbjct: 418 TAEGLVKL 425
>NP_001268425
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STADGLVEL 9
           +  DGL+EL
Sbjct: 230 TAGDGLIEL 238
>NP_733765
          Length = 1042

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_001672
          Length = 997

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_001273004
          Length = 869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 283 DGLVEL 288
>NP_001268644
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STADGLVEL 9
           +  DGL+EL
Sbjct: 230 TAGDGLIEL 238
>NP_002625
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   STADGLVEL 9
           +  DGL+EL
Sbjct: 230 TAGDGLIEL 238
>NP_004311
          Length = 994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_775293
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_777613
          Length = 1052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_777617
          Length = 998

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_777614
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_005164
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_777618
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_777615
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>XP_005256713
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>XP_005253945
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>XP_006713871
          Length = 530

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T +GLVEL
Sbjct: 160 NTYEGLVEL 168
>XP_006713870
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T +GLVEL
Sbjct: 399 NTYEGLVEL 407
>NP_203528
          Length = 767

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T +GLVEL
Sbjct: 397 NTYEGLVEL 405
>NP_777616
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   DGLVEL 9
           DGLVEL
Sbjct: 408 DGLVEL 413
>NP_001531
          Length = 205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TADGLVEL 9
           T DG+VE+
Sbjct: 113 TKDGVVEI 120
>NP_001107228
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 385 ADGTVEL 391
>NP_001107227
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 385 ADGTVEL 391
>NP_001944
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 385 ADGTVEL 391
>NP_001244917
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 385 ADGTVEL 391
>NP_005216
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TADGLVEL 9
           +ADG+V+L
Sbjct: 146 SADGVVDL 153
>NP_060390
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ADGLVEL 9
           +DGLVE+
Sbjct: 299 SDGLVEI 305
>NP_001071131
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ADGLVEL 9
           +DGLVE+
Sbjct: 308 SDGLVEI 314
>NP_001277005
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ADGLVEL 9
           +DGLVE+
Sbjct: 265 SDGLVEI 271
>NP_001277004
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ADGLVEL 9
           +DGLVE+
Sbjct: 265 SDGLVEI 271
>NP_001071132
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ADGLVEL 9
           +DGLVE+
Sbjct: 265 SDGLVEI 271
>NP_061870
          Length = 87

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TADGLVE 8
          T DGL+E
Sbjct: 31 TMDGLIE 37
>NP_114107
          Length = 87

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  TADGLVE 8
          T DGL+E
Sbjct: 31 TMDGLIE 37
>NP_001116108
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TADGLVEL 9
           +ADGL++L
Sbjct: 504 SADGLLQL 511
>NP_068580
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TADGLVEL 9
           +ADGL++L
Sbjct: 138 SADGLLQL 145
>NP_060733
          Length = 1435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 851 ADGTVEL 857
>NP_065880
          Length = 1423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T+DGL+ L
Sbjct: 158 ATSDGLLHL 166
>NP_001129392
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T+DGL+ L
Sbjct: 158 ATSDGLLHL 166
>NP_653213
          Length = 2211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ADGLVE 8
           ADGL+E
Sbjct: 403 ADGLIE 408
>NP_001193486
          Length = 1386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T+DGL+ L
Sbjct: 158 ATSDGLLHL 166
>XP_006722447
          Length = 1933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ADGLVE 8
           ADGL+E
Sbjct: 125 ADGLIE 130
>XP_005251580
          Length = 1422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   STADGLVEL 9
           +T+DGL+ L
Sbjct: 158 ATSDGLLHL 166
>XP_006712103
          Length = 1175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 591 ADGTVEL 597
>XP_006712102
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ADGLVEL 9
           ADG VEL
Sbjct: 729 ADGTVEL 735
>NP_115976
          Length = 1037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ADGLVEL 9
           +DGLVE+
Sbjct: 501 SDGLVEI 507
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.127    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 477,080
Number of extensions: 432
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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