Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1482
NP_001263689                                                           18    1482
NP_001119584                                                           18    1482
NP_001119586                                                           18    1482
NP_001263625                                                           18    1482
NP_001119590                                                           18    1482
NP_001263690                                                           18    1482
NP_001119585                                                           18    1482
NP_001263624                                                           18    1482
NP_005388                                                              16    5631
NP_001018121                                                           16    5631
NP_001269891                                                           16    7355
NP_060880                                                              16    7355
NP_001269892                                                           16    7355
NP_063940                                                              15    9605
NP_001265232                                                           15    9605
NP_620308                                                              15    9605
NP_001265233                                                           15    9605
NP_001123885                                                           15    9605
NP_064710                                                              15    9605
XP_005255493                                                           15    9605
XP_006721128                                                           15    9605
XP_005255492                                                           15    9605
NP_001005213                                                           15    9605
NP_001013376                                                           15    9605
>NP_000537
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SSV SQKTY
Sbjct: 95  SSVPSQKTY 103
>NP_001263689
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SSVLSQKTY 9
          SSV SQKTY
Sbjct: 56 SSVPSQKTY 64
>NP_001119584
          Length = 393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SSV SQKTY
Sbjct: 95  SSVPSQKTY 103
>NP_001119586
          Length = 341

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SSV SQKTY
Sbjct: 95  SSVPSQKTY 103
>NP_001263625
          Length = 302

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SSVLSQKTY 9
          SSV SQKTY
Sbjct: 56 SSVPSQKTY 64
>NP_001119590
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SSVLSQKTY 9
          SSV SQKTY
Sbjct: 56 SSVPSQKTY 64
>NP_001263690
          Length = 354

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SSVLSQKTY 9
          SSV SQKTY
Sbjct: 56 SSVPSQKTY 64
>NP_001119585
          Length = 346

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SSV SQKTY
Sbjct: 95  SSVPSQKTY 103
>NP_001263624
          Length = 307

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SSVLSQKTY 9
          SSV SQKTY
Sbjct: 56 SSVPSQKTY 64
>NP_005388
          Length = 526

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SSVLSQKT 8
           SSV+SQ+T
Sbjct: 237 SSVISQRT 244
>NP_001018121
          Length = 558

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SSVLSQKT 8
           SSV+SQ+T
Sbjct: 269 SSVISQRT 276
>NP_001269891
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SS++S KT+
Sbjct: 226 SSIISTKTF 234
>NP_060880
          Length = 748

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SS++S KT+
Sbjct: 280 SSIISTKTF 288
>NP_001269892
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SSVLSQKTY 9
           SS++S KT+
Sbjct: 195 SSIISTKTF 203
>NP_063940
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SSVLSQKTY 9
          S+ LSQ TY
Sbjct: 86 STFLSQTTY 94
>NP_001265232
          Length = 166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SSVLSQKTY 9
          S+ LSQ TY
Sbjct: 13 STFLSQTTY 21
>NP_620308
          Length = 186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SSVLSQKTY 9
          S+ LSQ TY
Sbjct: 33 STFLSQTTY 41
>NP_001265233
          Length = 186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SSVLSQKTY 9
          S+ LSQ TY
Sbjct: 33 STFLSQTTY 41
>NP_001123885
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 SVLSQKT 8
         +VLSQKT
Sbjct: 3 AVLSQKT 9
>NP_064710
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLSQKTY 9
           S +SQ+TY
Sbjct: 918 SAMSQETY 925
>XP_005255493
          Length = 959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLSQKTY 9
           S +SQ+TY
Sbjct: 825 SAMSQETY 832
>XP_006721128
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLSQKTY 9
           S +SQ+TY
Sbjct: 851 SAMSQETY 858
>XP_005255492
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SVLSQKTY 9
           S +SQ+TY
Sbjct: 892 SAMSQETY 899
>NP_001005213
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SSVLSQKTY 9
           SS++++KT+
Sbjct: 155 SSIITKKTF 163
>NP_001013376
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   SSVLSQKTY 9
           SS++++KT+
Sbjct: 155 SSIITKKTF 163
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.105    0.241 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 396,322
Number of extensions: 529
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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