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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1135
XP_006716142                                                           18    1135
XP_005250088                                                           18    1135
XP_006716141                                                           18    1135
XP_005250085                                                           18    1135
XP_006716140                                                           18    1135
XP_005250084                                                           18    1135
XP_005250083                                                           18    1135
XP_005250082                                                           18    1135
XP_006721791                                                           17    3301
NP_001001670                                                           16    7355
NP_997299                                                              16    7355
NP_001138669                                                           16    7355
XP_005276416                                                           16    7355
NP_005520                                                              15    9605
XP_005245920                                                           15    9605
XP_006710660                                                           15    9605
XP_006710659                                                           15    9605
XP_006710658                                                           15    9605
XP_006710657                                                           15    9605

>NP_733751
          Length = 4911

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4183 SSHGIAESA 4191



>XP_006716142
          Length = 4927

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4199 SSHGIAESA 4207



>XP_005250088
          Length = 4928

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4200 SSHGIAESA 4208



>XP_006716141
          Length = 4959

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4231 SSHGIAESA 4239



>XP_005250085
          Length = 4979

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4255 SSHGIAESA 4263



>XP_006716140
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4254 SSHGIAESA 4262



>XP_005250084
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4254 SSHGIAESA 4262



>XP_005250083
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4254 SSHGIAESA 4262



>XP_005250082
          Length = 4983

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SSHDIAESA 9
            SSH IAESA
Sbjct: 4255 SSHGIAESA 4263



>XP_006721791
          Length = 1078

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SSHDIAE 7
           SSHDIA+
Sbjct: 852 SSHDIAQ 858



>NP_001001670
          Length = 1576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HDIAES 8
           HD+AES
Sbjct: 462 HDLAES 467



>NP_997299
          Length = 917

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HDIAES 8
           HD+AES
Sbjct: 459 HDLAES 464



>NP_001138669
          Length = 917

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HDIAES 8
           HD+AES
Sbjct: 459 HDLAES 464



>XP_005276416
          Length = 1583

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HDIAES 8
           HD+AES
Sbjct: 448 HDLAES 453



>NP_005520
          Length = 4391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    SSHDIAES 8
            S H IAES
Sbjct: 4179 SGHGIAES 4186



>XP_005245920
          Length = 4392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    SSHDIAES 8
            S H IAES
Sbjct: 4180 SGHGIAES 4187



>XP_006710660
          Length = 4397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    SSHDIAES 8
            S H IAES
Sbjct: 4185 SGHGIAES 4192



>XP_006710659
          Length = 4556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    SSHDIAES 8
            S H IAES
Sbjct: 4344 SGHGIAES 4351



>XP_006710658
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    SSHDIAES 8
            S H IAES
Sbjct: 4351 SGHGIAES 4358



>XP_006710657
          Length = 4579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    SSHDIAES 8
            S H IAES
Sbjct: 4367 SGHGIAES 4374



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290    0.101    0.242 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,114
Number of extensions: 349
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)