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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     390
XP_006716142                                                           20     390
XP_005250088                                                           20     390
XP_006716141                                                           20     390
XP_005250085                                                           20     390
XP_006716140                                                           20     390
XP_005250084                                                           20     390
XP_005250083                                                           20     390
XP_005250082                                                           20     390
NP_003473                                                              18    1935
XP_006719679                                                           18    1935
XP_005269219                                                           18    1935
XP_006719678                                                           18    1935
XP_006719677                                                           18    1935
NP_001007560                                                           17    4312
NP_005628                                                              17    4312
XP_005258391                                                           17    4312
NP_443196                                                              15    9605
NP_079351                                                              15    9605
NP_066966                                                              15    9605
NP_001190179                                                           15    9605

>NP_733751
          Length = 4911

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4757 SKSSQYRKM 4765



>XP_006716142
          Length = 4927

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4773 SKSSQYRKM 4781



>XP_005250088
          Length = 4928

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4774 SKSSQYRKM 4782



>XP_006716141
          Length = 4959

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4805 SKSSQYRKM 4813



>XP_005250085
          Length = 4979

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4825 SKSSQYRKM 4833



>XP_006716140
          Length = 4982

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4828 SKSSQYRKM 4836



>XP_005250084
          Length = 4982

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4828 SKSSQYRKM 4836



>XP_005250083
          Length = 4982

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4828 SKSSQYRKM 4836



>XP_005250082
          Length = 4983

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+KM
Sbjct: 4829 SKSSQYRKM 4837



>NP_003473
          Length = 5537

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+++
Sbjct: 5383 SKSSQYRRL 5391



>XP_006719679
          Length = 5536

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+++
Sbjct: 5382 SKSSQYRRL 5390



>XP_005269219
          Length = 5537

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+++
Sbjct: 5383 SKSSQYRRL 5391



>XP_006719678
          Length = 5539

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+++
Sbjct: 5385 SKSSQYRRL 5393



>XP_006719677
          Length = 5540

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    SKSSQYQKM 9
            SKSSQY+++
Sbjct: 5386 SKSSQYRRL 5394



>NP_001007560
          Length = 418

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKSSQYQKM 9
          S+ SQYQ+M
Sbjct: 45 SECSQYQQM 53



>NP_005628
          Length = 387

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKSSQYQKM 9
          S+ SQYQ+M
Sbjct: 45 SECSQYQQM 53



>XP_005258391
          Length = 395

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKSSQYQKM 9
          S+ SQYQ+M
Sbjct: 22 SECSQYQQM 30



>NP_443196
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  SKSSQYQKM 9
          S + QYQ+M
Sbjct: 35 SATGQYQRM 43



>NP_079351
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  SQYQKM 9
          SQYQ+M
Sbjct: 48 SQYQRM 53



>NP_066966
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KSSQYQK 8
           KSS+YQ+
Sbjct: 131 KSSEYQE 137



>NP_001190179
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KSSQYQK 8
           KSS+YQ+
Sbjct: 128 KSSEYQE 134



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289   0.0954    0.216 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 379,861
Number of extensions: 277
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)