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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245202                                                           18    1935
NP_001245200                                                           18    1935
NP_001161091                                                           18    1935
NP_000240                                                              18    1935
NP_001161090                                                           18    1935
NP_001161089                                                           18    1935
NP_001245203                                                           18    1935
XP_005265221                                                           18    1935
XP_005265220                                                           18    1935
NP_000043                                                              17    4312
NP_001269153                                                           17    4312
NP_001013649                                                           16    5631
XP_006724707                                                           16    5631
NP_065753                                                              16    7355
XP_006716871                                                           16    7355

>NP_001245202
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SISLRKAIE 9
          S SLRKAIE
Sbjct: 28 STSLRKAIE 36



>NP_001245200
          Length = 687

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SISLRKAIE 9
           S SLRKAIE
Sbjct: 269 STSLRKAIE 277



>NP_001161091
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SISLRKAIE 9
          S SLRKAIE
Sbjct: 28 STSLRKAIE 36



>NP_000240
          Length = 756

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SISLRKAIE 9
           S SLRKAIE
Sbjct: 269 STSLRKAIE 277



>NP_001161090
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SISLRKAIE 9
          S SLRKAIE
Sbjct: 28 STSLRKAIE 36



>NP_001161089
          Length = 658

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SISLRKAIE 9
           S SLRKAIE
Sbjct: 171 STSLRKAIE 179



>NP_001245203
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SISLRKAIE 9
          S SLRKAIE
Sbjct: 28 STSLRKAIE 36



>XP_005265221
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SISLRKAIE 9
          S SLRKAIE
Sbjct: 28 STSLRKAIE 36



>XP_005265220
          Length = 515

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  SISLRKAIE 9
          S SLRKAIE
Sbjct: 28 STSLRKAIE 36



>NP_000043
          Length = 1500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SLRKAIE 9
           SLRKAIE
Sbjct: 330 SLRKAIE 336



>NP_001269153
          Length = 1422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SLRKAIE 9
           SLRKAIE
Sbjct: 330 SLRKAIE 336



>NP_001013649
          Length = 1225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   SISLRKA 7
           SISLRKA
Sbjct: 558 SISLRKA 564



>XP_006724707
          Length = 1337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   SISLRKA 7
           SISLRKA
Sbjct: 670 SISLRKA 676



>NP_065753
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SISLRKA 7
          S+SLRKA
Sbjct: 79 SVSLRKA 85



>XP_006716871
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SISLRKA 7
          S+SLRKA
Sbjct: 79 SVSLRKA 85



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.127    0.279 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 322,089
Number of extensions: 511
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)