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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001184037                                                           18    1935
NP_001184043                                                           18    1935
NP_001184042                                                           18    1935
NP_001184038                                                           18    1935
NP_001184036                                                           18    1935
NP_055390                                                              18    1935
NP_001073286                                                           17    4312
NP_000201                                                              17    4312
XP_006712574                                                           17    4312
XP_006712573                                                           17    4312
XP_005269218                                                           16    5631
NP_056290                                                              16    7355
XP_006726461                                                           16    7355
NP_002372                                                              15    9605

>NP_001184037
          Length = 255

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SIRQKTAIG 9
          SIRQK A+G
Sbjct: 29 SIRQKLALG 37



>NP_001184043
          Length = 536

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SIRQKTAIG 9
           SIRQK A+G
Sbjct: 310 SIRQKLALG 318



>NP_001184042
          Length = 563

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SIRQKTAIG 9
           SIRQK A+G
Sbjct: 337 SIRQKLALG 345



>NP_001184038
          Length = 244

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SIRQKTAIG 9
          SIRQK A+G
Sbjct: 18 SIRQKLALG 26



>NP_001184036
          Length = 474

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SIRQKTAIG 9
           SIRQK A+G
Sbjct: 248 SIRQKLALG 256



>NP_055390
          Length = 487

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SIRQKTAIG 9
           SIRQK A+G
Sbjct: 261 SIRQKLALG 269



>NP_001073286
          Length = 1091

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRQKTAIG 9
           +RQKTA G
Sbjct: 483 LRQKTACG 490



>NP_000201
          Length = 1073

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRQKTAIG 9
           +RQKTA G
Sbjct: 483 LRQKTACG 490



>XP_006712574
          Length = 1058

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRQKTAIG 9
           +RQKTA G
Sbjct: 483 LRQKTACG 490



>XP_006712573
          Length = 1076

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IRQKTAIG 9
           +RQKTA G
Sbjct: 483 LRQKTACG 490



>XP_005269218
          Length = 2009

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SIRQKTAI 8
          S+RQK+AI
Sbjct: 5  SLRQKSAI 12



>NP_056290
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SIRQKTA 7
           SIR+KTA
Sbjct: 285 SIREKTA 291



>XP_006726461
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIRQKTAIG 9
           ++RQKTA G
Sbjct: 274 TLRQKTAGG 282



>NP_002372
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SIRQKTAIG 9
           S+R K A+G
Sbjct: 389 SVRDKCALG 397



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.128    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 369,028
Number of extensions: 310
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)