Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1135
XP_006718908                                                           18    1135
XP_005271621                                                           18    1135
XP_006718907                                                           18    1135
XP_006718906                                                           18    1135
XP_005271619                                                           18    1135
XP_005271618                                                           18    1135
NP_000405                                                              16    7355
NP_001186221                                                           16    7355
NP_001186220                                                           16    7355
NP_644671                                                              15    9605
NP_009330                                                              15    9605
NP_001254514                                                           15    9605
NP_001254515                                                           15    9605
NP_699197                                                              15    9605
NP_006730                                                              15    9605
XP_006725233                                                           15    9605
XP_006725232                                                           15    9605
XP_006725231                                                           15    9605
XP_006724305                                                           15    9605
XP_006721485                                                           15    9605
XP_006721484                                                           15    9605
XP_006721483                                                           15    9605
XP_006720285                                                           15    9605
XP_005267988                                                           15    9605
XP_005267987                                                           15    9605
XP_006712781                                                           15    9605
NP_478144                                                              15    9605
>NP_000042
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SILEIISKS 9
            SILEI+SKS
Sbjct: 1403 SILEILSKS 1411
>XP_006718908
          Length = 1708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SILEIISKS 9
          SILEI+SKS
Sbjct: 55 SILEILSKS 63
>XP_005271621
          Length = 2708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SILEIISKS 9
            SILEI+SKS
Sbjct: 1055 SILEILSKS 1063
>XP_006718907
          Length = 3050

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SILEIISKS 9
            SILEI+SKS
Sbjct: 1397 SILEILSKS 1405
>XP_006718906
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SILEIISKS 9
            SILEI+SKS
Sbjct: 1403 SILEILSKS 1411
>XP_005271619
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SILEIISKS 9
            SILEI+SKS
Sbjct: 1403 SILEILSKS 1411
>XP_005271618
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SILEIISKS 9
            SILEI+SKS
Sbjct: 1403 SILEILSKS 1411
>NP_000405
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           SI+E++SK
Sbjct: 294 SIIEVLSK 301
>NP_001186221
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           SI+E++SK
Sbjct: 276 SIIEVLSK 283
>NP_001186220
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           SI+E++SK
Sbjct: 319 SIIEVLSK 326
>NP_644671
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SILEIISK 8
           SILE+I K
Sbjct: 560 SILELIKK 567
>NP_009330
          Length = 750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SILEIISK 8
           SILE+I K
Sbjct: 560 SILELIKK 567
>NP_001254514
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           S++EI+SK
Sbjct: 711 SLMEILSK 718
>NP_001254515
          Length = 999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           S++EI+SK
Sbjct: 658 SLMEILSK 665
>NP_699197
          Length = 3571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    SILEIISKS 9
            SILE +SK+
Sbjct: 2092 SILESVSKA 2100
>NP_006730
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SILEIISKS 9
           ++ E+ISKS
Sbjct: 330 NVYEVISKS 338
>XP_006725233
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 486 IIEIFSKS 493
>XP_006725232
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 486 IIEIFSKS 493
>XP_006725231
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 486 IIEIFSKS 493
>XP_006724305
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SILEIISKS 9
           ++ E+ISKS
Sbjct: 330 NVYEVISKS 338
>XP_006721485
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           S++EI+SK
Sbjct: 711 SLMEILSK 718
>XP_006721484
          Length = 997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           S++EI+SK
Sbjct: 656 SLMEILSK 663
>XP_006721483
          Length = 1061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILEIISK 8
           S++EI+SK
Sbjct: 740 SLMEILSK 747
>XP_006720285
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 486 IIEIFSKS 493
>XP_005267988
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 486 IIEIFSKS 493
>XP_005267987
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 486 IIEIFSKS 493
>XP_006712781
          Length = 750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SILEIISK 8
           SILE+I K
Sbjct: 560 SILELIKK 567
>NP_478144
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ILEIISKS 9
           I+EI SKS
Sbjct: 593 IIEIFSKS 600
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.127    0.268 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,710
Number of extensions: 366
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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