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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001276866                                                           18    1935
NP_004439                                                              18    1935
NP_001276865                                                           18    1935
NP_001005862                                                           18    1935
XP_005257197                                                           18    1935
XP_006721828                                                           18    1935
NP_666018                                                              17    3301
NP_001296                                                              15    9605
NP_006725                                                              15    9605
NP_001003760                                                           15    9605
XP_005267014                                                           15    9605
XP_005249164                                                           15    9605

>NP_001276866
          Length = 1055

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIISAAVGI 9
           SIISA VGI
Sbjct: 653 SIISAVVGI 661



>NP_004439
          Length = 1255

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIISAAVGI 9
           SIISA VGI
Sbjct: 653 SIISAVVGI 661



>NP_001276865
          Length = 1240

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIISAAVGI 9
           SIISA VGI
Sbjct: 638 SIISAVVGI 646



>NP_001005862
          Length = 1225

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIISAAVGI 9
           SIISA VGI
Sbjct: 623 SIISAVVGI 631



>XP_005257197
          Length = 1225

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIISAAVGI 9
           SIISA VGI
Sbjct: 623 SIISAVVGI 631



>XP_006721828
          Length = 1336

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIISAAVGI 9
           SIISA VGI
Sbjct: 734 SIISAVVGI 742



>NP_666018
          Length = 610

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IISAAVGI 9
          +ISAA+GI
Sbjct: 23 VISAAIGI 30



>NP_001296
          Length = 209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SIISAAVGI 9
          SII AA+G+
Sbjct: 87 SIIVAALGV 95



>NP_006725
          Length = 2446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIISAAVGI 9
           SI+S  VGI
Sbjct: 715 SIVSTPVGI 723



>NP_001003760
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   SIISAAV 7
           SIISAAV
Sbjct: 142 SIISAAV 148



>XP_005267014
          Length = 2446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIISAAVGI 9
           SI+S  VGI
Sbjct: 715 SIVSTPVGI 723



>XP_005249164
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   SIISAAV 7
           SIISAAV
Sbjct: 142 SIISAAV 148



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.134    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 254,950
Number of extensions: 187
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)