Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     509
NP_000242                                                              20     509
XP_005264389                                                           20     509
NP_006614                                                              16    5631
NP_065750                                                              16    7355
NP_000867                                                              16    7355
XP_005245421                                                           16    7355
XP_006711532                                                           16    7355
XP_005245420                                                           16    7355
NP_997244                                                              15    9605
NP_003391                                                              15    9605
NP_001118230                                                           15    9605
NP_000407                                                              15    9605
NP_065186                                                              15    9605
XP_006721512                                                           15    9605
XP_006721511                                                           15    9605
XP_006715534                                                           15    9605
XP_006715533                                                           15    9605
XP_005248177                                                           15    9605
XP_005248176                                                           15    9605
XP_005248175                                                           15    9605
XP_006712160                                                           15    9605
XP_006712159                                                           15    9605
XP_006712158                                                           15    9605
XP_005264603                                                           15    9605
XP_005264601                                                           15    9605
XP_006712157                                                           15    9605
>NP_001245210
          Length = 868

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SIIRSATKD 9
           SI+RSATKD
Sbjct: 668 SILRSATKD 676
>NP_000242
          Length = 934

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SIIRSATKD 9
           SI+RSATKD
Sbjct: 734 SILRSATKD 742
>XP_005264389
          Length = 921

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SIIRSATKD 9
           SI+RSATKD
Sbjct: 734 SILRSATKD 742
>NP_006614
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  IIRSATK 8
          I+RSATK
Sbjct: 52 IVRSATK 58
>NP_065750
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S+IRSAT
Sbjct: 246 SVIRSAT 252
>NP_000867
          Length = 2491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SIIRSATK 8
           S++RSAT+
Sbjct: 99  SVLRSATR 106
>XP_005245421
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S+IRSAT
Sbjct: 246 SVIRSAT 252
>XP_006711532
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S+IRSAT
Sbjct: 246 SVIRSAT 252
>XP_005245420
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S+IRSAT
Sbjct: 252 SVIRSAT 258
>NP_997244
          Length = 3169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SIIRSATKD 9
            SI+R  TKD
Sbjct: 2286 SIVRVHTKD 2294
>NP_003391
          Length = 1071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 667 SIIQQATKN 675
>NP_001118230
          Length = 549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IIRSATKD 9
           +IR +TKD
Sbjct: 485 LIRQSTKD 492
>NP_000407
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S++RSAT
Sbjct: 289 SVVRSAT 295
>NP_065186
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IIRSATKD 9
          +IR AT+D
Sbjct: 15 LIRGATRD 22
>XP_006721512
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IIRSATKD 9
           +IR +TKD
Sbjct: 315 LIRQSTKD 322
>XP_006721511
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IIRSATKD 9
           +IR +TKD
Sbjct: 334 LIRQSTKD 341
>XP_006715534
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S++RSAT
Sbjct: 248 SVVRSAT 254
>XP_006715533
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SIIRSAT 7
           S++RSAT
Sbjct: 279 SVVRSAT 285
>XP_005248177
          Length = 1399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IIRSATKD 9
          +IR AT+D
Sbjct: 15 LIRGATRD 22
>XP_005248176
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IIRSATKD 9
          +IR AT+D
Sbjct: 15 LIRGATRD 22
>XP_005248175
          Length = 1426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IIRSATKD 9
          +IR AT+D
Sbjct: 15 LIRGATRD 22
>XP_006712160
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 228 SIIQQATKN 236
>XP_006712159
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 500 SIIQQATKN 508
>XP_006712158
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 500 SIIQQATKN 508
>XP_005264603
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 536 SIIQQATKN 544
>XP_005264601
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 652 SIIQQATKN 660
>XP_006712157
          Length = 1071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SIIRSATKD 9
           SII+ ATK+
Sbjct: 667 SIIQQATKN 675
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.120    0.280 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 254,365
Number of extensions: 267
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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