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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           18    1135
NP_000170                                                              18    1135
NP_001268422                                                           18    1135
NP_001268423                                                           18    1135
XP_005264328                                                           18    1135
NP_078934                                                              16    7355
NP_001367                                                              15    9605
NP_996809                                                              15    9605
NP_065184                                                              15    9605
NP_037506                                                              15    9605
NP_001155901                                                           15    9605
NP_001243121                                                           15    9605

>NP_001268421
          Length = 1230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SELALSSLG 9
           SELALS+LG
Sbjct: 547 SELALSALG 555



>NP_000170
          Length = 1360

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SELALSSLG 9
           SELALS+LG
Sbjct: 677 SELALSALG 685



>NP_001268422
          Length = 1058

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SELALSSLG 9
           SELALS+LG
Sbjct: 375 SELALSALG 383



>NP_001268423
          Length = 1058

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SELALSSLG 9
           SELALS+LG
Sbjct: 375 SELALSALG 383



>XP_005264328
          Length = 1261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SELALSSLG 9
           SELALS+LG
Sbjct: 578 SELALSALG 586



>NP_078934
          Length = 1081

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ELALSSLG 9
           ELA+S+LG
Sbjct: 498 ELAVSALG 505



>NP_001367
          Length = 4646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ELALSSLG 9
            E ALSS+G
Sbjct: 1763 ETALSSMG 1770



>NP_996809
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ELALSSLG 9
          ELAL +LG
Sbjct: 62 ELALKTLG 69



>NP_065184
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  ELALSSLG 9
          ELAL +LG
Sbjct: 62 ELALKTLG 69



>NP_037506
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ELALSSLG 9
           +LAL+SLG
Sbjct: 110 KLALASLG 117



>NP_001155901
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ELALSSLG 9
           +LAL+SLG
Sbjct: 110 KLALASLG 117



>NP_001243121
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ELALSSLG 9
           +LAL+SLG
Sbjct: 110 KLALASLG 117



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.117    0.265 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 299,371
Number of extensions: 492
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)