Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1482
NP_009297                                                              18    1482
NP_115731                                                              16    5631
XP_006713408                                                           16    5631
NP_783322                                                              16    7355
NP_001265331                                                           16    7355
NP_001019818                                                           16    7355
NP_783324                                                              16    7355
NP_055031                                                              16    7355
NP_001136065                                                           16    7355
NP_001136066                                                           16    7355
NP_071901                                                              16    7355
NP_783323                                                              16    7355
XP_006719290                                                           16    7355
XP_006719289                                                           16    7355
NP_003631                                                              15    9605
NP_065988                                                              15    9605
NP_005024                                                              15    9605
NP_001029366                                                           15    9605
NP_002790                                                              15    9605
NP_060686                                                              15    9605
XP_005267986                                                           15    9605
XP_005252342                                                           15    9605
>NP_005148
          Length = 1130

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAVLEADKN 9
            SAVLEA KN
Sbjct: 1054 SAVLEAGKN 1062
>NP_009297
          Length = 1149

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAVLEADKN 9
            SAVLEA KN
Sbjct: 1073 SAVLEAGKN 1081
>NP_115731
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEADKN 9
           LEADKN
Sbjct: 289 LEADKN 294
>XP_006713408
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEADKN 9
           LEADKN
Sbjct: 192 LEADKN 197
>NP_783322
          Length = 476

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 358 SAVLEAVEN 366
>NP_001265331
          Length = 254

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 136 SAVLEAVEN 144
>NP_001019818
          Length = 254

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 136 SAVLEAVEN 144
>NP_783324
          Length = 411

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 358 SAVLEAVEN 366
>NP_055031
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLEADKN 9
          +LEAD+N
Sbjct: 64 ILEADQN 70
>NP_001136065
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLEADKN 9
          +LEAD+N
Sbjct: 64 ILEADQN 70
>NP_001136066
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  VLEADKN 9
          +LEAD+N
Sbjct: 64 ILEADQN 70
>NP_071901
          Length = 460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 342 SAVLEAVEN 350
>NP_783323
          Length = 395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 342 SAVLEAVEN 350
>XP_006719290
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 392 SAVLEAVEN 400
>XP_006719289
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAVLEADKN 9
           SAVLEA +N
Sbjct: 392 SAVLEAVEN 400
>NP_003631
          Length = 1332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AVLEADKN 9
            ++LEA KN
Sbjct: 1112 SILEAQKN 1119
>NP_065988
          Length = 2048

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SAVLEADKN 9
           SA+ + DKN
Sbjct: 432 SAIQQGDKN 440
>NP_005024
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLEADKN 9
           +LE DKN
Sbjct: 401 ILEPDKN 407
>NP_001029366
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLEADKN 9
           +LE DKN
Sbjct: 418 ILEPDKN 424
>NP_002790
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SAVLEAD 7
          +AVLEAD
Sbjct: 22 NAVLEAD 28
>NP_060686
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SAVLEAD 7
           SAVLE+D
Sbjct: 575 SAVLESD 581
>XP_005267986
          Length = 2022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SAVLEADKN 9
           SA+ + DKN
Sbjct: 406 SAIQQGDKN 414
>XP_005252342
          Length = 1218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AVLEADKN 9
            ++LEA KN
Sbjct: 998  SILEAQKN 1005
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294    0.111    0.258 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 455,015
Number of extensions: 721
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package