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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_705833                                                              16    7355
XP_005247174                                                           16    7355
NP_000072                                                              15    9605
NP_001135865                                                           15    9605
NP_006394                                                              15    9605
NP_001257962                                                           15    9605
XP_006711794                                                           15    9605
XP_005273086                                                           15    9605
XP_005273085                                                           15    9605
XP_005273084                                                           15    9605

>NP_705833
          Length = 2970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SASTRANLS 9
           S++T+AN+S
Sbjct: 787 SSNTKANMS 795



>XP_005247174
          Length = 2679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SASTRANLS 9
           S++T+AN+S
Sbjct: 496 SSNTKANMS 504



>NP_000072
          Length = 3801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SASTRANL 8
            SAST+A+L
Sbjct: 2832 SASTKADL 2839



>NP_001135865
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SASTRANLS 9
           + STR+N+S
Sbjct: 373 TGSTRSNMS 381



>NP_006394
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SASTRANLS 9
           + STR+N+S
Sbjct: 373 TGSTRSNMS 381



>NP_001257962
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SASTRANLS 9
           + STR+N+S
Sbjct: 224 TGSTRSNMS 232



>XP_006711794
          Length = 3022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SASTRANL 8
            SAST+A+L
Sbjct: 2053 SASTKADL 2060



>XP_005273086
          Length = 3264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SASTRANL 8
            SAST+A+L
Sbjct: 2832 SASTKADL 2839



>XP_005273085
          Length = 3755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SASTRANL 8
            SAST+A+L
Sbjct: 2786 SASTKADL 2793



>XP_005273084
          Length = 3801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SASTRANL 8
            SAST+A+L
Sbjct: 2832 SASTKADL 2839



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288   0.0932    0.203 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 239,782
Number of extensions: 192
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)