Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     665
NP_001035957                                                           19     665
XP_006721991                                                           19     665
XP_006721990                                                           19     665
XP_006721989                                                           19     665
XP_005258041                                                           19     665
XP_006721988                                                           19     665
XP_006721987                                                           19     665
XP_006721986                                                           19     665
XP_005258040                                                           19     665
XP_006721985                                                           19     665
NP_660333                                                              17    4312
NP_149039                                                              17    4312
NP_079272                                                              17    4312
XP_006713858                                                           17    4312
NP_055318                                                              16    5631
XP_006719406                                                           16    5631
NP_061744                                                              16    7355
NP_114478                                                              16    7355
NP_000236                                                              15    9605
NP_001120972                                                           15    9605
NP_079235                                                              15    9605
XP_006719671                                                           15    9605
XP_006719670                                                           15    9605
XP_006716054                                                           15    9605
XP_006716053                                                           15    9605
XP_006716052                                                           15    9605
XP_006716051                                                           15    9605
NP_835462                                                              15    9605
NP_001004460                                                           15    9605
>NP_000258
          Length = 2818

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1386 SAMFLRFIN 1394
>NP_001035957
          Length = 2839

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1407 SAMFLRFIN 1415
>XP_006721991
          Length = 1632

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1417 SAMFLRFIN 1425
>XP_006721990
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1417 SAMFLRFIN 1425
>XP_006721989
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1417 SAMFLRFIN 1425
>XP_005258041
          Length = 2836

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1386 SAMFLRFIN 1394
>XP_006721988
          Length = 2846

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1396 SAMFLRFIN 1404
>XP_006721987
          Length = 2849

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1417 SAMFLRFIN 1425
>XP_006721986
          Length = 2854

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1404 SAMFLRFIN 1412
>XP_005258040
          Length = 2857

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1407 SAMFLRFIN 1415
>XP_006721985
          Length = 2867

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SAMFLTFIN 9
            SAMFL FIN
Sbjct: 1417 SAMFLRFIN 1425
>NP_660333
          Length = 1321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMFLTFIN 9
           A F+TF+N
Sbjct: 924 ASFITFVN 931
>NP_149039
          Length = 334

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SAMFLTFIN 9
           S +FLTFI+
Sbjct: 109 SILFLTFIS 117
>NP_079272
          Length = 751

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FLTFIN 9
           FLTFIN
Sbjct: 169 FLTFIN 174
>XP_006713858
          Length = 712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FLTFIN 9
           FLTFIN
Sbjct: 169 FLTFIN 174
>NP_055318
          Length = 2785

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SAMFLTFIN 9
           S +FL FIN
Sbjct: 843 SPLFLRFIN 851
>XP_006719406
          Length = 2380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SAMFLTFIN 9
           S +FL FIN
Sbjct: 843 SPLFLRFIN 851
>NP_061744
          Length = 932

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAMFLTFI 8
           S +FLTF+
Sbjct: 701 SCVFLTFV 708
>NP_114478
          Length = 828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAMFLTFI 8
           S +FLTF+
Sbjct: 701 SCVFLTFV 708
>NP_000236
          Length = 1390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SAMFLTFI 8
            SA+F TFI
Sbjct: 1338 SAIFSTFI 1345
>NP_001120972
          Length = 1408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SAMFLTFI 8
            SA+F TFI
Sbjct: 1356 SAIFSTFI 1363
>NP_079235
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMFLTFI 8
           A+FLTF+
Sbjct: 212 AVFLTFL 218
>XP_006719671
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMFLTFI 8
           A+FLTF+
Sbjct: 212 AVFLTFL 218
>XP_006719670
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AMFLTFI 8
          A+FLTF+
Sbjct: 17 AVFLTFL 23
>XP_006716054
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SAMFLTFI 8
           SA+F TFI
Sbjct: 908 SAIFSTFI 915
>XP_006716053
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SAMFLTFI 8
           SA+F TFI
Sbjct: 908 SAIFSTFI 915
>XP_006716052
          Length = 1409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SAMFLTFI 8
            SA+F TFI
Sbjct: 1357 SAIFSTFI 1364
>XP_006716051
          Length = 1427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SAMFLTFI 8
            SA+F TFI
Sbjct: 1375 SAIFSTFI 1382
>NP_835462
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  SAMFLTFI 8
          S +FLTF+
Sbjct: 26 SLLFLTFL 33
>NP_001004460
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  SAMFLTFI 8
          S +FLTF+
Sbjct: 12 SLLFLTFL 19
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.142    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 256,410
Number of extensions: 388
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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